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Gene expression profiles of Setd2 fl/fl BMSCs infected with lentivirus expressing GFP and Cre respectively

ABSTRACT: Gene expression profiles of Setd2 fl/fl BMSCs infected with lentivirus expressing GFP and Cre respectively

PROVIDER: PRJNA492290 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7882340.fastq.gz	Fastqsanger.gz
	SRR7882350.fastq.gz	Fastqsanger.gz
	SRR7882341.fastq.gz	Fastqsanger.gz
	SRR7882351.fastq.gz	Fastqsanger.gz
	SRR7882342.fastq.gz	Fastqsanger.gz

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Similar Datasets

Gene expression profiles of Setd2 fl/fl BMSCs infected with lentivirus expressing GFP and Cre respectively

Project description:During the aging process, bone marrow mesenchymal stem cells (BMSCs) exhibit declined osteogenesis accompanied by excess adipogenesis, which will lead to osteoporosis. Here we report that the H3K36 trimethylation, catalyzed by histone methyltransferase SETD2 regulates lineage commitment of BMSCs. Deletion of Setd2 in mBMSCs, through conditional Cre expression driven by Prx1 promoter, resulted in bone loss and marrow adiposity. Loss of Setd2 in BMSCs in vitro facilitated differentiation propensity to adipocytes rather than to osteoblasts. Through conjoint analysis of RNA-seq and ChIP-seq data, we identified a SETD2 functional target gene, Lbp, on which H3K36me3 was enriched, and its expression was affected by Setd2 deficiency. Furthermore, overexpression of LBP could partially rescue the lack of osteogenesis and enhanced adipogenesis resulted from the absence of Setd2 in BMSCs. Further mechanism study demonstrated that the trimethylation level of H3K36 could regulate Lbp transcriptional initiation and elongation. These findings suggest that H3K36 trimethylation mediated by SETD2 could regulate the cell fate of mesenchymal stem cells in vitro and in vivo, indicating that the regulation of H3K36me3 level by targeting SETD2 and/or the administration of downstream LBP protein may represent potential therapeutic way for new treatment in metabolic bone diseases, such as osteoporosis.

2018-11-08 | GSE120244 | GEO

H3K36me3 ChIP profiles of Setd2 fl/fl BMSCs infected with lentivirus expressing GFP and Cre

Project description:H3K36me3 ChIP profiles of Setd2 fl/fl BMSCs infected with lentivirus expressing GFP and Cre

| PRJNA492879 | ENA

H3K36me3 ChIP profiles of Setd2 fl/fl BMSCs infected with lentivirus expressing GFP and Cre

2018-11-08 | GSE120351 | GEO

Gene expression and ChIP profiles of Setd2 fl/fl BMSCs infected with lentivirus expressing GFP and Cre

Project description:Gene expression and ChIP profiles of Setd2 fl/fl BMSCs infected with lentivirus expressing GFP and Cre

| PRJNA492881 | ENA

Gene expression and ChIP profiles of Setd2 fl/fl BMSCs infected with lentivirus expressing GFP and Cre

Project description:This SuperSeries is composed of the SubSeries listed below.

2018-11-08 | GSE120361 | GEO

Effect of overexpression of FNDC5 on gene expression upon hypoxia 24 h of bone marrow-mesenchymal stem cells (BMSCs).

Project description:To investigate the function of FNDC5 in the regulation of autophagy, we established BMSCs-OE-FNDC5 in which the target gene FNDC5 has been overexpressed by lentivirus transfection.

2023-09-13 | GSE231837 | GEO

Global transcriptome profiling of SETD2 and hnRNP L depleted cells and H3K36me3 profile of WT 293T cells

Project description:We performed RNA-seq of 293T cells post depletion and SETD2 or hnRNP L to compare their global transcriptome profile. We also looked at the distribution of the histone mark H3K36me3 in wild type 293T to correlate it with the observed transcriptome changes upon SETD2 and hnRNP L depletion. We rescued SETD2 knock out 293T cells with SETD2 FL (Full Length), FLΔSRI (FLwoSRI) and FLΔSHI (FLwoSHI) and performed H3K36me3 ChIP-Seq.

2021-01-29 | GSE151296 | GEO

Genome-wide map of chromatin states and global transcriptome profiling in SETD2 wild type and mutant cells

Project description:We looked at the distribution of the histone mark H3K36me3 in wild type 293T and SETD2 deleted 293T (KO) cells. We also looked at the H3K36me3 profile after rescuing the KO cells with recombinant SETD2 constructs; FL (full length), N3 (1404-2564) and N3ΔSRI/N3woSRI (1404-2468). Furthermore, we performed RNA-Seq of cells expressing the wild type or mutant/recombinant copy of SETD2 to compare their global transcriptome profile.

2021-03-25 | GSE147752 | GEO

genes differentially expressed in bone marrow mesenchymal stem cells (BMSCs) between the control mice (rhebFL/FL) and rheb BAD KO mice (Ucp1-cre; rhebFL/FL)

Project description:RNA sequencing was performed to analyze expression profile in BMSCs from mice with or without BAT-specific rheb knockout.

2021-10-21 | GSE186092 | GEO

Expression data from human bone marrow derived mesenchymal stem cells (BMSCs) either cultured alone or co-cultured with human colon cancer cells HT29 or HCT116 respectively

Project description:The cross-talk between mesenchymal stem cells (MSCs) and cancer cells remains controversial. It is imperative to understand how MSC and tumor cells communicate with each other and whether the MSC responds to the tumor according to its stage of progression. We used microarrays to detail the global programme of gene expression in human BMSCs either cultured alone or co-cultured with colon cancer cells HT29 or HCT116 respectively

2017-10-31 | GSE106325 | GEO

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