Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq, ChIP-seq and single cell RNA-seq of human skin Langerhans cells

ABSTRACT: RNA-seq, ChIP-seq and single cell RNA-seq of human skin Langerhans cells

PROVIDER: PRJNA492925 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR10355438_1.fastq.gz	Fastqsanger.gz
	SRR10355438_2.fastq.gz	Fastqsanger.gz
	SRR10355439_1.fastq.gz	Fastqsanger.gz
	SRR10355439_2.fastq.gz	Fastqsanger.gz
	SRR10355440_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 44

Similar Datasets

RNA-seq, ChIP-seq and single cell RNA-seq of human skin Langerhans cells

Project description:Langerhans cells (LCs) in the epidermis promote immune homeostasis, efficiently activating tolerogenic and immunogenic T cell responses. To understand genomic programming in human Langerhans cells we performed whole transcriptome (bulk RNA-seq and single cell RNA-seq) profiling and analysis of H3K4Me3 and H3K27Ac histone modifications across LC genome in primary human cells from 6 independent donors. Primary LCs were either unstimulated and stimulated with TNF-alpha. Additionally we performed a CRISPR editing experiment for IRF4

2019-12-16 | GSE120386 | GEO

PD-L1 Enhanced by cis-Urocanic Acid on Langerhans Cells Inhibits Vg4+ gdT17 Cells in Psoriatic Skin Inflammation

Project description:To establish the role of PD-L1 expression on Langerhans cells and its influence on psoriatic skin inflammation, we isolated Langerhans cells from harvested ears of wild type mice treated with Vaseline or Imiquimod. We then determined the gene expression profile of isolated Langerhans cells from the different groups at a single time point using data from RNA-seq. The experiment was performed in duplicate.

2023-06-30 | GSE222197 | GEO

Comparison of skin and in vitro differentiated human Langerhans cells

Project description:Langerhans cells were prepared from skin or derived from cord blood CD34+ cells

2021-03-04 | GSE168167 | GEO

Identification of ChIP-seq and RIME grade antibodies for Estrogen Receptor alpha

Project description:Estrogen Receptor alpha (ERα) is a key driver of most breast cancers, and it is the target of endocrine therapies used in the clinic to treat women with ERα positive (ER+) breast cancer. The two methods ChIP-seq (chromatin immunoprecipitation coupled with deep sequencing) and RIME (Rapid Immunoprecipitation of Endogenous Proteins) have greatly improved our understanding of ERα function during breast cancer progression and in response to anti-estrogens. A critical component of both ChIP-seq and RIME protocols is the antibody that is used to pull down the bait protein. To date, most of the ChIP-seq and RIME experiments for the study of ERα have been performed using the sc-543 antibody from Santa Cruz Biotechnology. However, this antibody has been discontinued, thereby severely impacting the study of ERα in normal physiology as well as diseases such as breast cancer and ovarian cancer. Here, we compare the sc-543 antibody with other commercially available antibodies, and we show that 06-935 (EMD Millipore) and ab3575 (Abcam) antibodies can successfully replace the sc-543 antibody for ChIP-seq and RIME experiments.

2019-03-21 | PXD012930 | Pride

Gene array analysis of langerhans cells and macrophages from human skin and pDCs from human spleen

Project description:Langerhans cells and macrophages were isolated from adult skin that was collagenase digeseted overnight by FACS. Adult spleen was collagenase digested for 1hr and the pDCs were isolated by FACS. Gene expression analysis using total RNA from specific cell subsets was performed.

2020-01-21 | GSE122673 | GEO

Transcription profiling of mouse Langerhans cells from aryl hydrocarbon receptor deficient mice

Project description:The arylhydrocarbon receptor is a ligand inducible transcription factor. Known to control xenobiotic metabolizing enzymes, it also affects - depending on the cell type - numerous other genes, either directly or indirectly. With respect to the immune system, persistent activation leads to immunosuppression. We asked how the AhR is involved in Langerhans cells. These antigen presenting cells of the skin are responsible for allergies against chemicals (thus xenobiotic metabolism might be relevant) and a recently detected endogenous ligand, FICZ made by UVB radiation from tryptophane, is particularly abundant in the skin. Experiment Overall Design: ear skin epidermal sheets from AhR exon2 deficient mice and wildtype C57BL/6 mice were isolated and cultivated for 16 hours in standard medium. Tissues were then homogenized and Langerhans cells isolated via BSA gradient and cell sorting to greater 95% purity. RNA was preparedand amplified prior to chip-hybridization using the MessageAmpâ¢ Kit of Ambion (Woodward St. Austin, USA). RNA was biotinylated (Enzo Bio Arrayâ¢HighYieldâ¢ RNA transcript labeling kit (Affymetrix, High Wycombe, UK)) and purified. RNA was hybridized to MOE430A gene chips (Affymetrix).

2009-11-16 | E-GEOD-13130 | biostudies-arrayexpress

Langerhans cell RNAseq

Project description:CD45.1 bone marrow cells were transplanted into irradiated CD45.2 host to generate bone marrow chimera mice. Skin langerhans cells and other dendritic cell subsets were sorted from chimera mice and sequenced.

2021-03-22 | GSE139877 | GEO

Convergent evolution of MDP-monocyte differentiation in adult skin permits repair of the Langerhans cell network [RNA-seq]

Project description:Langerhans cells (LCs) are a unique population of phagocytes programmed within embryonic skin to maintain tissue and immunological homeostasis at the epidermal barrier site. Unique amongst tissue-resident macrophages, LCs play roles in skin innervation and repair, while also functioning as migrating professional antigen presenting cells, a capability classically assigned to dendritic cells (DC). We current lack insight into the molecular pathways that determine this dichotomy, when they are established, and whether precursors recruited to the adult skin niche access the same instructive signals laid down in utero. Tracking differentiation of monocyte-derived (m)LCs after destruction of embryo-derived cells in murine adult skin, revealed intrinsic intra-epidermal heterogeneity and selection of MDP-monocytes to by-pass gene programmes specifying a macrophage fate. Adopting a process unique to adult skin, subsequent extrinsic specification of EpCAM+ precursors via follicular Notch ligands, and aryl hydrocarbon receptor (Ahr) signalling, combined to determine commitment to resident mLCs. Using a unique human LC dataset, we demonstrated that embryo-derived (e)LCs in newborn human skin retained transcriptional evidence of their macrophage origin, which was superseded by a distinct DC-like immune modules following proliferation in infant skin. Thus, convergent differentiation of monocytes within adult skin exploits novel molecular pathways to replicate conditioning of eLC towards more immunogenic DC-like cells within post-natal skin.

2024-09-08 | GSE247874 | GEO

Convergent evolution of MDP-monocyte differentiation in adult skin permits repair of the Langerhans cell network [RNA-seq]

Project description:Convergent evolution of MDP-monocyte differentiation in adult skin permits repair of the Langerhans cell network [RNA-seq]

| PRJNA1040849 | ENA

RNA-seq, YY1 ChIP-seq and MeDIP-seq of radiation-induced skin fibrosis of rats

Project description:RNA-seq, YY1 ChIP-seq and MeDIP-seq of radiation-induced skin fibrosis of rats

| PRJNA480033 | ENA