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Next Generation Sequencing Facilitates Quantitative Analysis of Dura macrophage Transcriptomes post adoptive T cell reconstitution of RAG-1 deficient mice

ABSTRACT: Next Generation Sequencing Facilitates Quantitative Analysis of Dura macrophage Transcriptomes post adoptive T cell reconstitution of RAG-1 deficient mice

PROVIDER: PRJNA493130 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7903260.fastq.gz	Fastqsanger.gz
	SRR7903270.fastq.gz	Fastqsanger.gz
	SRR7903280.fastq.gz	Fastqsanger.gz
	SRR7903310.fastq.gz	Fastqsanger.gz
	SRR7903320.fastq.gz	Fastqsanger.gz

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Next Generation Sequencing Facilitates Quantitative Analysis of Dura macrophage Transcriptomes post adoptive T cell reconstitution of RAG-1 deficient mice II

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of Dura macrophage Transcriptomes post adoptive T cell reconstitution of RAG-1 deficient mice II

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Next Generation Sequencing Facilitates Quantitative Analysis of Dura macrophage Transcriptomes post adoptive T cell reconstitution of RAG-1 deficient mice I

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of Dura macrophage Transcriptomes post adoptive T cell reconstitution of RAG-1 deficient mice I

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Next Generation Sequencing Facilitates Quantitative Analysis of Dura macrophage Transcriptomes post adoptive T cell reconstitution of RAG-1 deficient mice

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Next Generation Sequencing Facilitates Quantitative Analysis of Dura macrophage Transcriptomes post adoptive T cell reconstitution of RAG-1 deficient mice II

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RNA-seq analysis of pro-B cells from RAG, double RAG/Emu, double RAG/3'RR and double RAG/KOKI deficient mice.

Project description:By their impact on nuclear organisation, enhancers are master regulators of cell fate. We thus developed double Emu-RAG-deficient, 3’RR-RAG-deficient mice and KOKI-RAG-deficient mice to investigate a potential transcriptional cross-talk between Emu and 3'RR enhancers at the pro-B cell stage.

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Differential gene expression between juvenile and adult dura mater

Project description:BACKGROUND: Although reossification of large calvarial defects is possible in children, adults lack this tissue engineering capacity. In this study, the authors compared the differences in gene expression between juvenile and adult dura mater using a mouse cDNA microarray with 42,000 unique elements. METHODS: Non-suture-associated parietal bone was harvested from 6-day-old and 60-day-old mice. The dura mater was carefully dissected from the calvarial disk and snap-frozen. RNA was extracted from pooled dura mater for microarray analysis. The 25 most differentially expressed genes were listed, as were selected bone-related genes. In addition, quantitative real-time reverse-transcriptase polymerase chain reaction confirmation of selected genes-BMP-2, BMP-4, and BMP-7; and osteopontin (OP), osteocalcin (OC), and FGFR-1-was performed. : Juvenile dura mater expressed significantly greater amounts of BMP-2 and OP. Minimal difference in OC expression was observed between juvenile and adult dura mater. Extracellular matrix proteins (Col3a1, 5a1, 6a1, and fibronectin 1), osteoblast differentiation markers (Runx2/Cbfa1, Itm2a, and FGFR-1), and the growth factor Ptn were among other genes with greater expression in juvenile dura mater. Markers of osteoclasts (Acp5, MMP9, Ctsk) and the multiple candidate gene Ntrk2 were also expressed at higher levels in the juvenile dura mater. CONCLUSIONS: These findings suggest a more differentiated osteoprogenitor population to exist along with a greater presence of osteoclasts in the juvenile dura mater relative to adults. In addition to establishing a baseline difference in gene expression between juvenile and adult dura mater, new genes potentially critical to the regenerative potential of juvenile calvaria were identified. Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. Keywords: Logical Set

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2019-10-11 | PXD015778 | Pride

Transcription profiling of mouse adult and juvenile dura mater (bone) to explore the reossification of large calvarial defects during development

Project description:BACKGROUND: Although reossification of large calvarial defects is possible in children, adults lack this tissue engineering capacity. In this study, the authors compared the differences in gene expression between juvenile and adult dura mater using a mouse cDNA microarray with 42,000 unique elements. METHODS: Non-suture-associated parietal bone was harvested from 6-day-old and 60-day-old mice. The dura mater was carefully dissected from the calvarial disk and snap-frozen. RNA was extracted from pooled dura mater for microarray analysis. The 25 most differentially expressed genes were listed, as were selected bone-related genes. In addition, quantitative real-time reverse-transcriptase polymerase chain reaction confirmation of selected genes-BMP-2, BMP-4, and BMP-7; and osteopontin (OP), osteocalcin (OC), and FGFR-1-was performed. RESULTS: Juvenile dura mater expressed significantly greater amounts of BMP-2 and OP. Minimal difference in OC expression was observed between juvenile and adult dura mater. Extracellular matrix proteins (Col3a1, 5a1, 6a1, and fibronectin 1), osteoblast differentiation markers (Runx2/Cbfa1, Itm2a, and FGFR-1), and the growth factor Ptn were among other genes with greater expression in juvenile dura mater. Markers of osteoclasts (Acp5, MMP9, Ctsk) and the multiple candidate gene Ntrk2 were also expressed at higher levels in the juvenile dura mater. CONCLUSIONS: These findings suggest a more differentiated osteoprogenitor population to exist along with a greater presence of osteoclasts in the juvenile dura mater relative to adults. In addition to establishing a baseline difference in gene expression between juvenile and adult dura mater, new genes potentially critical to the regenerative potential of juvenile calvaria were identified.

2006-11-06 | E-SMDB-3845 | biostudies-arrayexpress

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