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Next Generation Sequencing Facilitates Quantitative Analysis of Dura macrophage Transcriptomes post adoptive T cell reconstitution of RAG-1 deficient mice II

ABSTRACT: Next Generation Sequencing Facilitates Quantitative Analysis of Dura macrophage Transcriptomes post adoptive T cell reconstitution of RAG-1 deficient mice II

PROVIDER: PRJNA493131 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7903310.fastq.gz	Fastqsanger.gz
	SRR7903320.fastq.gz	Fastqsanger.gz
	SRR7903311.fastq.gz	Fastqsanger.gz
	SRR7903321.fastq.gz	Fastqsanger.gz
	SRR7903312.fastq.gz	Fastqsanger.gz

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Next Generation Sequencing Facilitates Quantitative Analysis of Dura macrophage Transcriptomes post adoptive T cell reconstitution of RAG-1 deficient mice

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of Dura macrophage Transcriptomes post adoptive T cell reconstitution of RAG-1 deficient mice

| PRJNA493130 | ENA

Next Generation Sequencing Facilitates Quantitative Analysis of Dura macrophage Transcriptomes post adoptive T cell reconstitution of RAG-1 deficient mice I

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of Dura macrophage Transcriptomes post adoptive T cell reconstitution of RAG-1 deficient mice I

| PRJNA493129 | ENA

Next Generation Sequencing Facilitates Quantitative Analysis of Dura macrophage Transcriptomes post adoptive T cell reconstitution of RAG-1 deficient mice

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-09-25 | GSE120486 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of Dura macrophage Transcriptomes post adoptive T cell reconstitution of RAG-1 deficient mice II

Project description:The goal of this study was to compare transcriptional changes evoked by T cells cross talk with meningeal macrophages. Furthermore, the role of specific T cell subsets or selected cytokine deficiency were used to evaluate their role in regulating such transcriptional changes

2021-09-25 | GSE120485 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of Dura macrophage Transcriptomes post adoptive T cell reconstitution of RAG-1 deficient mice I

2021-09-25 | GSE120483 | GEO

RNA-seq analysis of pro-B cells from RAG, double RAG/Emu, double RAG/3'RR and double RAG/KOKI deficient mice.

Project description:By their impact on nuclear organisation, enhancers are master regulators of cell fate. We thus developed double Emu-RAG-deficient, 3’RR-RAG-deficient mice and KOKI-RAG-deficient mice to investigate a potential transcriptional cross-talk between Emu and 3'RR enhancers at the pro-B cell stage.

2019-02-07 | GSE117449 | GEO

Differential gene expression between juvenile and adult dura mater

Project description:BACKGROUND: Although reossification of large calvarial defects is possible in children, adults lack this tissue engineering capacity. In this study, the authors compared the differences in gene expression between juvenile and adult dura mater using a mouse cDNA microarray with 42,000 unique elements. METHODS: Non-suture-associated parietal bone was harvested from 6-day-old and 60-day-old mice. The dura mater was carefully dissected from the calvarial disk and snap-frozen. RNA was extracted from pooled dura mater for microarray analysis. The 25 most differentially expressed genes were listed, as were selected bone-related genes. In addition, quantitative real-time reverse-transcriptase polymerase chain reaction confirmation of selected genes-BMP-2, BMP-4, and BMP-7; and osteopontin (OP), osteocalcin (OC), and FGFR-1-was performed. : Juvenile dura mater expressed significantly greater amounts of BMP-2 and OP. Minimal difference in OC expression was observed between juvenile and adult dura mater. Extracellular matrix proteins (Col3a1, 5a1, 6a1, and fibronectin 1), osteoblast differentiation markers (Runx2/Cbfa1, Itm2a, and FGFR-1), and the growth factor Ptn were among other genes with greater expression in juvenile dura mater. Markers of osteoclasts (Acp5, MMP9, Ctsk) and the multiple candidate gene Ntrk2 were also expressed at higher levels in the juvenile dura mater. CONCLUSIONS: These findings suggest a more differentiated osteoprogenitor population to exist along with a greater presence of osteoclasts in the juvenile dura mater relative to adults. In addition to establishing a baseline difference in gene expression between juvenile and adult dura mater, new genes potentially critical to the regenerative potential of juvenile calvaria were identified. Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. Keywords: Logical Set

2006-10-03 | GSE5950 | GEO

S. mansoni gene expression 21-27d post-infection in wt and RAG-/- mice

Project description:RNA isolated from S. mansoni worms grown in C57BL/6 or RAG-/- mice at 21, 23, 25 and 27 days post-infection was hybridized to a S. mansoni custom microarray. Expression was compared to a reference pool containing equal amounts of RNA from each condition and time point assayed. Keywords: Time course with two growth conditions (wt and RAG-/- mice)

2007-06-28 | GSE8254 | GEO

Architecture of human Rag GTPase heterodimers and their complex with mTORC1

Project description:The Rag GTPases recruit the master kinase mTORC1 to lysosomes to regulate cell growth and proliferation in response to amino acid availability. The nucleotide state of Rag heterodimers is critical for their association with mTORC1. Our cryo-EM structure of RagA/RagC in complex with mTORC1 shows the details of RagA/C binding to the RAPTOR subunit of mTORC1 and explains why only the RagAGTP/RagCGDP nucleotide state binds mTORC1. Previous kinetic studies suggested that GTP binding to one Rag locks the heterodimer to prevent GTP binding to the other. Our crystal structures and dynamics show the mechanism for this locking, and explain how oncogenic hotspot mutations disrupt this process. In contrast to allosteric activation by RHEB, Rag heterodimer binding does not change mTORC1 conformation and activates mTORC1 by targeting it to lysosomes.

2019-10-11 | PXD015778 | Pride

Transcription profiling of mouse adult and juvenile dura mater (bone) to explore the reossification of large calvarial defects during development

Project description:BACKGROUND: Although reossification of large calvarial defects is possible in children, adults lack this tissue engineering capacity. In this study, the authors compared the differences in gene expression between juvenile and adult dura mater using a mouse cDNA microarray with 42,000 unique elements. METHODS: Non-suture-associated parietal bone was harvested from 6-day-old and 60-day-old mice. The dura mater was carefully dissected from the calvarial disk and snap-frozen. RNA was extracted from pooled dura mater for microarray analysis. The 25 most differentially expressed genes were listed, as were selected bone-related genes. In addition, quantitative real-time reverse-transcriptase polymerase chain reaction confirmation of selected genes-BMP-2, BMP-4, and BMP-7; and osteopontin (OP), osteocalcin (OC), and FGFR-1-was performed. RESULTS: Juvenile dura mater expressed significantly greater amounts of BMP-2 and OP. Minimal difference in OC expression was observed between juvenile and adult dura mater. Extracellular matrix proteins (Col3a1, 5a1, 6a1, and fibronectin 1), osteoblast differentiation markers (Runx2/Cbfa1, Itm2a, and FGFR-1), and the growth factor Ptn were among other genes with greater expression in juvenile dura mater. Markers of osteoclasts (Acp5, MMP9, Ctsk) and the multiple candidate gene Ntrk2 were also expressed at higher levels in the juvenile dura mater. CONCLUSIONS: These findings suggest a more differentiated osteoprogenitor population to exist along with a greater presence of osteoclasts in the juvenile dura mater relative to adults. In addition to establishing a baseline difference in gene expression between juvenile and adult dura mater, new genes potentially critical to the regenerative potential of juvenile calvaria were identified.

2006-11-06 | E-SMDB-3845 | biostudies-arrayexpress

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