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INDUCTION OF HUMAN MESENCHYMAL STEM CELLS FROM NEONATAL AND ADULT SOURCES BY A SMALL MOLECULE COCKTAIL

ABSTRACT: INDUCTION OF HUMAN MESENCHYMAL STEM CELLS FROM NEONATAL AND ADULT SOURCES BY A SMALL MOLECULE COCKTAIL

PROVIDER: PRJNA494089 | ENA |

REPOSITORIES: ENA

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INDUCTION OF HUMAN MESENCHYMAL STEM CELLS FROM NEONATAL AND ADULT SOURCES BY A SMALL MOLECULE COCKTAIL

Project description:Human mesenchymal stem cells (MSCs) are good candidates for brain cell-replacement strategies and has been already used as adjuvant treatment in neurological disorders. MSCs can be obtained from many different sources, and the present study compares the neuronal potential transdifferentiation of MSCs from adult and neonatal sources with a protocol previously tested, consisting in a cocktail of six small molecules: I-BET151, CHIR99021, Forskolin, RepSox, Y-27632, and dbcAMP (ICFRYA). A neuronal morphology and the presence of cells positive to neuronal markers (TUJ-1 and MAP2) were considered attributes of neuronal induction. The present study showed marked differences between MSCs from different sources in response to the transdifferentiation protocol here used. These results may contribute to identifying the best source of MSCs for potential cell-replacement therapies.

2018-10-02 | GSE120681 | GEO

Induction of mouse totipotent stem cells by a defined chemical cocktail

Project description:Induction of mouse totipotent stem cells by a defined chemical cocktail

| PRJNA767352 | ENA

Induction of mouse totipotent stem cells by a defined chemical cocktail [RRBS]

Project description:Induction of mouse totipotent stem cells by a defined chemical cocktail [RRBS]

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Neuronal Transdifferentiation Potential of Human Mesenchymal Stem Cells from Neonatal and Adult Sources by a Small Molecule Cocktail.

Project description:Human mesenchymal stem cells (MSCs) are good candidates for brain cell replacement strategies and have already been used as adjuvant treatments in neurological disorders. MSCs can be obtained from many different sources, and the present study compares the potential of neuronal transdifferentiation in MSCs from adult and neonatal sources (Wharton's jelly (WhJ), dental pulp (DP), periodontal ligament (PDL), gingival tissue (GT), dermis (SK), placenta (PLAC), and umbilical cord blood (UCB)) with a protocol previously tested in bone marrow- (BM-) MSCs consisting of a cocktail of six small molecules: I-BET151, CHIR99021, forskolin, RepSox, Y-27632, and dbcAMP (ICFRYA). Neuronal morphology and the presence of cells positive for neuronal markers (TUJ1 and MAP2) were considered attributes of neuronal induction. The ICFRYA cocktail did not induce neuronal features in WhJ-MSCs, and these features were only partial in the MSCs from dental tissues, SK-MSCs, and PLAC-MSCs. The best response was found in UCB-MSCs, which was comparable to the response of BM-MSCs. The addition of neurotrophic factors to the ICFRYA cocktail significantly increased the number of cells with complex neuron-like morphology and increased the number of cells positive for mature neuronal markers in BM- and UCB-MSCs. The neuronal cells generated from UCB-MSCs and BM-MSCs showed increased reactivity of the neuronal genes TUJ1, MAP2, NF-H, NCAM, ND1, TAU, ENO2, GABA, and NeuN as well as down- and upregulation of MSC and neuronal genes, respectively. The present study showed marked differences between the MSCs from different sources in response to the transdifferentiation protocol used here. These results may contribute to identifying the best source of MSCs for potential cell replacement therapies.

| S-EPMC6466843 | biostudies-literature

A core of differentially methylated CpG loci in gMDSCs isolated from neonatal and adult sources

Project description:Using the Illumina EPIC array to measure DNAm of gMDSCs and neutrophils from diverse neonatal and adult blood sources, we found 189 differentially methylated CpGs between gMDSCs and neutrophils with a core of ten differentially methylated CpGs that were consistent across both sources of cells. Genes associated with these loci that are involved in immune responses include VCL, FATS, YAP1, KREMEN2, UBTF, MCC-1, and EFCC1. In two cancer patient groups that reflected those used to develop the methylation markers (head and neck squamous cell carcinoma (HNSCC) and glioma), all of the CpG loci were differentially methylated, reaching statistical significance in glioma cases and controls, while one was significantly different in the smaller HNSCC group. Our findings indicate that gMDSCs have a core of distinct DNAm alterations, informing future research on gMDSC differentiation and function.

2023-05-01 | GSE231417 | GEO

Induction of mouse totipotent stem cells by a defined chemical cocktail [scRNA-seq]

Project description:Induction of mouse totipotent stem cells by a defined chemical cocktail [scRNA-seq]

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Induction of mouse totipotent stem cells by a defined chemical cocktail [ATAC-seq]

Project description:Induction of mouse totipotent stem cells by a defined chemical cocktail [ATAC-seq]

| PRJNA767349 | ENA

Induction of mouse totipotent stem cells by a defined chemical cocktail [bulk RNA-seq]

Project description:Induction of mouse totipotent stem cells by a defined chemical cocktail [bulk RNA-seq]

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Chemical induction of hepatocyte revitalization in mouse hepatocytes [RNA-seq 2]

Project description:We examined how each chemical contributed to hepatocyte revitalization by removing each component of the 5C induction cocktail individually. We then compared deach chemical contributed to hepatocyte revitalization by removing each component of the 5C induction cocktail individually

2023-11-21 | GSE233082 | GEO

Supplementary Dataset 75d - N terminomics of Jurkat lysate - apoptotic - cocktail

Project description:This dataset contains N-terminal peptides that were modified by a stabiligase cocktail in Jurkat (human) lysate following induction of apoptosis with etoposide.

2017-07-12 | MSV000081313 | MassIVE

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