Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: Beta catenin knock out in hESC

PROVIDER: PRJNA494223 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR7947403.fastq.gz	Fastqsanger.gz
	SRR7947404.fastq.gz	Fastqsanger.gz

Items per page:

1 - 2 of 2

Similar Datasets

Canonical Wnt pathway controls mESCs self-renewal through inhibition of spontaneous differentiation via β-catenin/TCF/LEF functions

Project description:The Wnt/β-catenin signalling pathway is a key regulator of embryonic stem cell self-renewal and differentiation. Constitutive activation of this pathway has been shown to significantly increase mouse embryonic stem cell (mESC) self-renewal and pluripotency marker expression. In this study, we generated a novel β-catenin knock-out model in mESCs by using CRISPR/Cas9 technology to delete putatively functional N-terminally truncated isoforms observed in previous knock-out models. While we showed that aberrant N-terminally truncated isoforms are not functional in mESCS, we observed that canonical Wnt signalling is not active in mESCs, as β-catenin ablation does not alter mESC transcriptional profile in LIF-enriched culture conditions; on the other hand, Wnt signalling activation represses mESC spontaneous differentiation. We also showed that transcriptionally silent β-catenin (ΔC) isoforms can rescue β-catenin knock-out self-renewal defects in mESCs, cooperating with TCF1 and LEF1 in the inhibition of mESC spontaneous differentiation in a Gsk3 dependent manner.

2020-01-10 | GSE143340 | GEO

E-MTAB-2439

Project description:miR-206/133b knock-out

2014-07-03 | E-MTAB-2439 | ExpressionAtlas

Transcription profiling of mouse conditional knock out of beta-catenin by K19-CRE at E7.5

Project description:expression profile of conditional knock out of beta-catenin by K19-CRE at E7.5. Tested a wild type with two alleles of beta-catenin, a heterzyote with one deleted allele and the conditional null in the domain on cytokeratin 19 driven CRE expression

2008-06-12 | E-GEOD-2519 | biostudies-arrayexpress

Expression profile of conditional knock out of beta-catenin by K19-CRE at E7.5

2005-04-13 | GSE2519 | GEO

E-MTAB-7013

Project description:RNA-seq of Drosophila melanogaster testis with or without tbrd-1 knock out or tplus3a/b knock out

2019-05-14 | E-MTAB-7013 | ExpressionAtlas

β-catenin regulates primitive streak induction through collaborative interactions with SMAD2/3 and OCT4

Project description:Canonical Wnt and Nodal signaling are both required for induction of the primitive streak (PS), which guides organization of the early embryo. The Wnt effector β-catenin is thought to function in these early lineage specification decisions via transcriptional activation of Nodal signaling. Here, we demonstrate a broader role for β-catenin in PS formation by analyzing its genome-wide binding in a human embryonic stem cell model of PS induction. β-catenin occupies regulatory regions in numerous PS and neural crest genes, and direct interactions between β-catenin and the Nodal effectors SMAD2/3 are required at these regions for PS gene activation. Furthermore, OCT4 binding in proximity to these sites is likewise required for PS induction, suggesting a collaborative interaction between β-catenin and OCT4. Induction of neural crest genes by β-catenin is repressed by SMAD2/3, ensuring proper lineage specification. This study provides mechanistic insight into how Wnt signaling controls early cell lineage decisions. Examination of β-catenin binding in hESC incubated in media control (RPMI), media containing CHIR or CHIR+SB for 6h and analyzed by ChIP-sequencing

2015-03-31 | E-GEOD-58476 | biostudies-arrayexpress

β-catenin mediates endodermal commitment of human ES cells via distinct transactivation functions

Project description:Activation of Wnt/β-catenin signaling is pivotal to the formation of definitive endoderm (DE) lineage during early embryonic development, but its regulatory mechanism was not yet fully understood. In this study, we generated new CTNNB1-/- human ES cells (hESC) through CRISPR-based insertional gene disruption approach and performed systematic rescue using various truncated or mutant β-catenin to study the Wnt/β-catenin-centered cellular mechanisms underlying DE differentiation from hESC. We used RNA-seq to examine the transcriptional information to understand the functions of β-catenin.

2024-12-31 | GSE222125 | GEO

E-MTAB-2561

Project description:myocardial infarction in Reg3beta knock-out mice

2015-07-27 | E-MTAB-2561 | ExpressionAtlas

RNA-seq analysis of HSF1 knockout on LiCl treatment

Project description:Heat shock transcription factor 1(HSF1) is an important transcription factor which regulates the expression of a wide array of genes including heat shock proteins and oncogenes. Here, we report that HSF1 as a target of WNT/β-catenin signaling, regulates parts of target genes of WNT/β-catenin signaling. To explore the biological relevance of HSF1 activation to WNT/β-catenin signaling, we profiled gene expression of wild type mouse embryonic fibroblasts (WT MEF) and HSF1 knock out MEF (HSF1 KO MEF) before and after lithium chloride (LiCl) treatment which was a potent GSK3β inhibitor and increased the expression of β-catenin.

2020-08-31 | GSE151119 | GEO

Homo sapiens

Project description:Gene Expression analysis of hESC B2M knock-out

| PRJNA179552 | ENA