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Genome-wide profiling of Scc1 subunit in wild type cells and in cells in which chromatin structure is perturbed (histone H4 depletion).

ABSTRACT: Genome-wide profiling of Scc1 subunit in wild type cells and in cells in which chromatin structure is perturbed (histone H4 depletion).

PROVIDER: PRJNA495336 | ENA |

REPOSITORIES: ENA

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Analysis by Mnase-seq of the chromatin structure of wild type cells and cells in which chromatin structure is perturbed (histone H4 depletion) in the presence or absence of the kleisin subunit of cohesin, Scc1

Project description:Analysis by Mnase-seq of the chromatin structure of wild type cells and cells in which chromatin structure is perturbed (histone H4 depletion) in the presence or absence of the kleisin subunit of cohesin, Scc1

| PRJNA495502 | ENA

Mapping the differential distribution of the cohesin subunit Scc1 in Trypanosoma brucei brucei.

Project description:Chromatin immunoprecipitation of genomic loci in Trypanosoma brucei where Scc1 is deposited. This was achieved by deletion of one Scc1 allele and N-terminal tagging of the second Scc1 allele with a Ty1-tag. During the ChIP experiment, the DNA was crosslinked and cells were permeabilized with digitonin. DNA was fragmented by sonication with a Covaris instrument. DNA bound to Ty1-Scc1 was pulled down by using a BB2 anti-Ty1 antibody. Cross links are reversed and the DNA was purified and prepared for Illumina sequencing.

2018-11-06 | GSE119071 | GEO

Genome-wide profiling of Scc1 subunit in wild type cells and in cells in which chromatin structure is perturbed (histone H4 depletion).

Project description:The main objective of this study is to determine how chromatin assembly during DNA replication influences cohesin deposition

2019-08-06 | GSE121004 | GEO

Wapl-mediated release of Scc1-cohesin regulates chromosome structure and segregation in mouse oocytes

Project description:Cohesin is essential for genome folding and chromosome inheritance through cell divisions. In somatic cells, both of these functions are mediated by Scc1-cohesin, which in mitosis is released from chromosomes by Wapl and separase. In mammalian oocytes, cohesion is mediated by Rec8-cohesin. Scc1 is also expressed but neither required nor sufficient for cohesion, and its function in oocytes is unknown. Likewise, it is unknown whether Wapl regulates one or both cohesin complexes and chromosome segregation in mature oocytes. Using conditional mouse mutagenesis, we show that in meiosis I Wapl is required for proper chromosome segregation, predominantly releases Scc1-cohesin from chromosomes and promotes production of euploid eggs. Using single-nucleus Hi-C, we found that Scc1 is essential for chromatin loops and topologically associating domains (TADs) in oocytes. Increasing Scc1 residence time on chromosomes by Wapl depletion leads to vermicelli formation and intra-loop structures but, unlike in mitotic cells, does not increase loop size. This implies that loop size has reached a maximum in oocytes, possibly because it is limited by barriers such as cohesive cohesin. We conclude that distinct cohesin complexes generate loops and cohesion in mammalian oocytes and propose that the same principle applies to all cell types and species.

2020-03-04 | GSE132264 | GEO

Scc1 ChIP-Seq in a strain where the centromere on chromosome III has been moved to an ectopic site

Project description:Genome binding/occupancy profiling of the cohesin subunit Scc1 by high throughput sequencing

2020-01-13 | GSE104082 | GEO

Calibrated Scc1 ChIP-seq for wild type and rad61Δ strains arrested in metaphase with and without tension

Project description:Genome binding/occupancy profiling of the cohesin subunit Scc1 by high throughput sequencing. S. pombe is included as the calibration genome.

2020-01-13 | GSE128225 | GEO

Calibrated Scc1 ChIP-seq for wild type and chl4Δ strains arrested in metaphase with and without tension

Project description:Genome binding/occupancy profiling of the cohesin subunit Scc1 by high throughput sequencing. S. pombe is included as the calibration genome.

2020-01-13 | GSE128238 | GEO

Mapping the differential distribution of the cohesin subunit Scc1 in Trypanosoma brucei brucei.

Project description:Mapping the differential distribution of the cohesin subunit Scc1 in Trypanosoma brucei brucei.

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Project description:The main objective of this study is to determine how cohesin influences chromatin structure and chromatin assembly during DNA replication

2019-08-06 | GSE121067 | GEO

Scc2 Is a Potent Activator of Cohesin's ATPase that Promotes Loading by Binding Scc1 without Pds5

Project description:In addition to sharing with condensin an ability to organize DNA into chromatids, cohesin regulates enhancer-promoter interactions and confers sister chromatid cohesion. Association with chromosomes is regulated by hook-shaped HEAT repeat proteins that Associate With its Kleisin (Scc1) subunit (HAWKs), namely Scc3, Pds5, and Scc2. Unlike Pds5, Scc2 is not a stable cohesin constituent but, as shown here, transiently displaces Pds5 during loading. Scc1 mutations that compromise its interaction with Scc2 adversely affect cohesin’s ATPase activity, loading, and translocation while Scc2 mutations that alter how the ATPase responds to DNA abolish loading despite cohesin’s initial association with loading sites. Lastly, Scc2 mutations that permit loading in the absence of Scc4 increase Scc2’s association with chromosomal cohesin and reduce that of Pds5. We suggest that cohesin switches between two states, one with Pds5 bound to Scc1 that is not able to hydrolyse ATP efficiently but is capable of release from chromosomes and another in which Scc2, transiently replacing Pds5, stimulates the ATP hydrolysis necessary for loading and translocation away from loading sites.

2018-07-17 | GSE106182 | GEO

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