RNAseq of fluidically isolated human and mouse motor neuron somas and axons in vitro
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA495499 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR7993060.fastq.gz | Fastqsanger.gz | |||
| SRR7993070.fastq.gz | Fastqsanger.gz | |||
| SRR7993080.fastq.gz | Fastqsanger.gz | |||
| SRR7993090.fastq.gz | Fastqsanger.gz | |||
| SRR7993100.fastq.gz | Fastqsanger.gz |
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Project description:The motor neuron (MN)–hexamer complex consisting of LIM homeobox 3, Islet-1, and nuclear LIM interactor is a key determinant of motor neuron specification and differentiation. To gain insights into the transcriptional network in motor neuron development, we performed a genome-wide ChIP-sequencing analysis and found that the MN–hexamer directly regulates a wide array of motor neuron genes by binding to the HxRE (hexamer response element) shared among the target genes. Interestingly, STAT3-binding motif is highly enriched in the MN–hexamer–bound peaks in addition to the HxRE. We also found that a transcriptionally active form of STAT3 is expressed in embryonic motor neurons and that STAT3 associates with the MN–hexamer, enhancing the transcriptional activity of the MN–hexamer in an upstream signal-dependent manner. Correspondingly, STAT3 was needed for motor neuron differentiation in the developing spinal cord. Together, our studies uncover crucial gene regulatory mechanisms that couple MN–hexamer and STAT-activating extracellular signals to promote motor neuron differentiation in vertebrate spinal cord.
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