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Profiling Kif5A RNA targets in sensory neuron axons

ABSTRACT: Profiling Kif5A RNA targets in sensory neuron axons

PROVIDER: PRJNA595899 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10703860_1.fastq.gz	Fastqsanger.gz
	SRR10703860_2.fastq.gz	Fastqsanger.gz
	SRR10703861_1.fastq.gz	Fastqsanger.gz
	SRR10703861_2.fastq.gz	Fastqsanger.gz
	SRR10703862_1.fastq.gz	Fastqsanger.gz

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Profiling Kif5A RNA targets in sensory neuron axons

Project description:We previously identified that the RNA binding protein Nucleolin is localized to axons of DRG sensory neurons by interaction with the molecular motor Kinesin-1 (Kif5A) and subsequently localizes importin beta1 mRNA there (Perry et al., 2016). To further identify additional RNAs that are localized to axons in a similar mechanism, we immunopercipitated Kif5A from dorsal roots (centrally projecting axons) or Sciatic nerves (peripherally projecting axons), isolated the bound RNA and sequnced it.

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