TCR sequencing of Treg and Tconv subsets from murine spleen
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA495846 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR8034240_1.fastq.gz | Fastqsanger.gz | |||
| SRR8034240_2.fastq.gz | Fastqsanger.gz | |||
| SRR8034250_1.fastq.gz | Fastqsanger.gz | |||
| SRR8034250_2.fastq.gz | Fastqsanger.gz | |||
| SRR8034241_1.fastq.gz | Fastqsanger.gz |
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Project description:While unique subsets of Treg cells have been described in some non-lymphoid tissues, their relationship to Treg cells in secondary lymphoid organs and circulation remains unclear. We have identified a recirculating and highly suppressive effector Treg cell subset that expresses the α2 integrin, CD49b, and exhibits a unique tissue distribution. To identify genes and pathways enriched in CD49b+ Treg cells, we performed RNA-seq of splenic CD49b+ and CD49b− Treg cells that were of otherwise similar activation status based on expression of CD44 and CD62L. This revealed that splenic CD49b+ Treg cells express genes related to migration and activation, but are relatively depleted of genes whose expression is TCR-dependent in Treg cells. These results shed light on the identity and development of a functionally potent subset of mature effector Treg cells that recirculates through and surveys peripheral tissues.
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