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Contrasting roles of transcription factors spineless and EcR in the highly dynamic chromatin landscape of butterfly wing metamorphosis

ABSTRACT: Contrasting roles of transcription factors spineless and EcR in the highly dynamic chromatin landscape of butterfly wing metamorphosis

PROVIDER: PRJNA498282 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8086570_1.fastq.gz	Fastqsanger.gz
	SRR8086570_2.fastq.gz	Fastqsanger.gz
	SRR8086580_1.fastq.gz	Fastqsanger.gz
	SRR8086580_2.fastq.gz	Fastqsanger.gz
	SRR8086590_1.fastq.gz	Fastqsanger.gz

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Contrasting roles of transcription factors spineless and EcR in the highly dynamic chromatin landscape of butterfly wing metamorphosis

Project description:Background: We studied the chromatin accessibility landscsape in wings during butterfly metamorphosis, and investigate which transcription factors might be driving changes in accessibility Methods: We sequencing the Junonia coenia genome, and we studied chromatin accessibility using ATAC seq in multiple stages of wing development in both forewings and hindwings. For sites showing a large change in accessibility, we investigate which motifs are enriched, and correlate this with changes in gene expression of associated transcription factors. We confirm promising candidates with ChIP-seq Results: We find a highly dynamic landscape, with multiple peaks showing a double increase in accessibility throughout development. We show that transcription factor spineless, but not ecdysone receptor, is highly predictive of opening sites Conclusions: This work provides a characterization of the chromatin dynamics of insect wing metamorphosis, identifies novel candidate chromatin remodeling factors in insects, and provides the first genome assembly of the model butterfly Junonia coenia, with gene and cis-regulatory element annotations

2019-04-30 | GSE121734 | GEO

Contrasting roles of transcription factors spineless and EcR in the highly dynamic chromatin landscape of butterfly wing metamorphosis

2019-04-30 | GSE121541 | GEO

Contrasting roles of transcription factors spineless and EcR in the highly dynamic chromatin landscape of butterfly wing metamorphosis

2019-05-07 | GSE121542 | GEO

Contrasting roles of transcription factors spineless and EcR in the highly dynamic chromatin landscape of butterfly wing metamorphosis

Project description:Contrasting roles of transcription factors spineless and EcR in the highly dynamic chromatin landscape of butterfly wing metamorphosis

| PRJNA497879 | ENA

Contrasting roles of transcription factors spineless and EcR in the highly dynamic chromatin landscape of butterfly wing metamorphosis

Project description:Contrasting roles of transcription factors spineless and EcR in the highly dynamic chromatin landscape of butterfly wing metamorphosis

| PRJNA497878 | ENA

Contrasting roles of transcription factors spineless and EcR in the highly dynamic chromatin landscape of butterfly wing metamorphosis

Project description:Contrasting roles of transcription factors spineless and EcR in the highly dynamic chromatin landscape of butterfly wing metamorphosis

| PRJNA498283 | ENA

Contrasting roles of transcription factors spineless and EcR in the highly dynamic chromatin landscape of butterfly wing metamorphosis

Project description:This SuperSeries is composed of the SubSeries listed below.

2019-04-30 | GSE121735 | GEO

Identification of genes dependent on the Ecdysone receptor (EcR) at the onset of metamorphosis in Drosophila

Project description:This study identifies those genes that are dependent on EcR for their proper regulation at the onset of metamorphosis in Drosophila melanogaster. Keywords: Nuclear receptor gene regulation

2005-10-04 | GSE3069 | GEO

Opportunistic binding of EcR to open chromatin drives tissue-specific developmental responses [CUT&RUN]

Project description:In Drosophila, the ecdysone steroid hormone is essential for coordinating developmental timing across physically separated tissues. Ecdysone directly impacts genome function through its receptor, a heterodimer of the EcR and Usp proteins. Ligand binding to EcR triggers a transcriptional cascade, including activation of a set of primary response transcription factors. The hierarchical organization of this pathway has left the direct role of EcR in mediating ecdysone responses obscured. Here, we investigate the role of EcR in controlling tissue-specific ecdysone responses, focusing on two tissues that diverge in their response to rising ecdysone titers: the larval salivary gland, which undergoes programmed destruction, and the wing imaginal disc, which initiates metamorphosis. We find that EcR functions bimodally, with both gene repressive and activating functions, even at the same developmental stage. We find that EcR DNA binding profiles are highly tissue-specific, and transgenic reporter analyses demonstrate that EcR plays a direct role in controlling enhancer activity. Despite a strong correlation between tissue-specific EcR binding and tissue-specific open chromatin, we find that EcR does not control chromatin accessibility at genomic targets. We conclude that EcR contributes extensively to tissue-specific ecdysone responses. However, control over access to its binding sites is subordinated to other transcription factors.

2022-09-12 | GSE202807 | GEO

Opportunistic binding of EcR to open chromatin drives tissue-specific developmental responses [FAIRE-seq]

2022-09-12 | GSE202808 | GEO