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Antimony and arsenic contanmination in in rice paddies during different stages of growth

ABSTRACT: antimony and arsenic contanmination in in rice paddies during different stages of growth Raw sequence reads

PROVIDER: PRJNA498333 | ENA |

REPOSITORIES: ENA

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Project description:The mRNA expression of antimony resistant strains of Leishmania donovani was compared to the expression of the sensitive Leishmania donovani. The antimony resistant and sensitive Leishmania donovani were grown in complete M199 medium with 10% FCS and Penicillin streptomycin mixture. At stationary phase (5 day culture) cells were harvested in sterile Phosphate buffered saline and used for RNA isolation.

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Transcriptional analysis of Leishmania infantum antimony resistant strains using full-genome DNA microarrays

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GENE EXPRESSION PROFILING AND MOLECULAR CHARACTERIZATION OF ANTIMONY RESISTANCE IN Leishmania amazonensis

Project description:Background: Drug resistance is a major problem in leishmaniasis chemotherapy. RNA expression profiling using DNA microarrays is a suitable approach to study simultaneous events leading to a drug-resistance phenotype. Genomic analysis has been performed primarily with Old World Leishmania species and here we investigate molecular alterations in antimony resistance in the New World species L. amazonensis. Methods/Principal Findings: We selected populations of L. amazonensis for resistance to antimony by step-wise drug pressure. Gene expression of highly resistant mutants was studied using DNA microarrays. RNA expression profiling of antimony-resistant L. amazonensis revealed the overexpression of genes involved in drug resistance including the ABC transporter MRPA and several genes related to thiol metabolism. The MRPA overexpression was validated by quantitative real-time PCR and further analysis revealed that this increased expression was correlated to gene amplification as part of extrachromosomal linear amplicons in some mutants and as part of supernumerary chromosomes in other mutants. The expression of several other genes encoding hypothetical proteins but also nucleobase and glucose transporter encoding genes were found to be modulated. Conclusions/Significance: Mechanisms classically found in Old World antimony resistant Leishmania were also highlighted in New World antimony-resistant L. amazonensis. These studies were useful to the identification of resistance molecular markers.

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Microarray based differential transcriptomics study to understand arsenic-thiourea interaction in rice.

Project description:In our earlier study, thiourea has been shown to improve the growth of rice and reduce the arsenic load from aerial parts of the seedlings. We have also shown the applicability of thiourea to delineate the redox regulatory mechansisms under arsenic stress in rice. To move further, present microarray was performed to reveal the involvement of overall gene and regulatory netwrok under arsenic with/without thiourea treatment in rice.

2015-09-15 | GSE71492 | GEO

Overall gene expression comparison between antimony resistant and sensitive Leishmania donovani

Project description:The mRNA expression of antimony resistant strains of Leishmania donovani was compared to the expression of the sensitive Leishmania donovani.

2016-01-01 | GSE62468 | GEO

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