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Transcriptional analysis of Leishmania infantum antimony resistant strains using full-genome DNA microarrays

ABSTRACT: The aim of the experiment is to detect any gene expression modulation in an antimony resistant L.infantum Sb2000.1 strain compared to an antimony sensitive L.infantum WT strain. We want to identify the modulated genes associated with an antimony resistant phenotype. The Sb2000.1 resistant strain used is about 1000 times more resistant to SbIII than the WT sensitive strain. The full-genome DNA microarrays includes one 70-oligonucleotide probe for each gene of L. infantum. Keywords: drug resistance Two-condition experiment, resistant strain Sb2000.1 vs wild-type. Five biological replicates for each strain, independently grown and harvested. One replicate per array

ORGANISM(S): Leishmania infantum

SUBMITTER: Philippe Leprohon

PROVIDER: E-GEOD-16965 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression modulation is associated with gene amplification, supernumerary chromosomes and chromosome loss in antimony-resistant Leishmania infantum.

Leprohon Philippe P Légaré Danielle D Raymond Frédéric F Madore Eric E Hardiman Gary G Corbeil Jacques J Ouellette Marc M

Nucleic acids research 20090107 5

Antimonials remain the first line drug against the protozoan parasite Leishmania but their efficacy is threatened by resistance. We carried out a RNA expression profiling analysis comparing an antimony-sensitive and -resistant (Sb2000.1) strain of Leishmania infantum using whole-genome 70-mer oligonucleotide microarrays. Several genes were differentially expressed between the two strains, several of which were found to be physically linked in the genome. MRPA, an ATP-binding cassette (ABC) gene ...[more]

PMID: 19129236

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