Project description:We report the MNase-diestion coupled to Next Generation Sequencing of Wild type Drosophila S2 cells or S2 cells over-expressing polycomb protein PH
Project description:RNA-Seq was used In order to evaluate the global transcriptomic changes of CycA dsRNA and Myb dsRNA induced endoreplicating cells (iECs) in DrosophilaS2-DGRC cells. CycA dsRNA and Myb dsRNA iECs mRNA levels were compared to control mitotic cycling cells treated in parallel with GFP dsRNA, in three biological replicates. The RNA-Seq results indicated that a switch from mitotic cycles to endoreplication is associated with up and down regulation of thousands of genes. Comparison of the CycA dsRNA and Myb dsRNA iEC transcriptomes revealed that they shared a total of 966 genes that were differentially expressed compared to mitotic cycling controls
Project description:Shavenbaby (Svb) transcription factor is involved in the differenciation of epidermal cells, the homeostasis of intestinal stem cell. Svb is first synthetized as a long repressor protein called SvbRepressor (SvbREP). In presence of Pri peptides Svb Repressor is clived by the proteasoome into a shorter activator protein called Svb Activator (SvbACT). We want in this study determine the effect of the two Svb forms on gene expression and determine the Svb target genes. We performed RNAseq experiment in S2 cell lines which present Svb REP or SvbACT or S2 cell without Svb.
Project description:High-throughput sequencing of Drosophila melanogaster small RNAs from S2 cells. total RNA, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf
Project description:Shavenbaby (Svb) transcription factor is involved in the differenciation of epidermal cells, the homeostasis of intestinal stem cell. Svb is first synthetized as a long repressor protein called SvbRepressor (SvbREP). In presence of Pri peptides Svb Repressor is clived by the proteasoome into a shorter activator protein called Svb Activator (SvbACT). We want in this study determine the binding behaviour of the two Svb forms on S2 cells. We performed ChIPseq Svb experiment in S2 cell lines which present Svb REP or SvbACT or S2 cell without Svb.
Project description:Brucellosis is a zoonotic disease caused by Gram-negative bacteria, resulting in an economic loss of billions of USD per year. Most of the Brucellosis vaccines in the application are the whole bacteria vaccines, which typically have high toxicity and low immunogenicity. We recently developed an S2 vaccine adjuvanted with Ag85A (Ag85A-S2), which greatly improved the immunogenic properties of S2. However, the mechanisms of Ag85A-S2 remained elusive. Here, we found that Ag85A-S2 activated cGAS-STING pathways both in intestinal mucosal cells and in a cGAS knockout cell line in vitro. We demonstrated that the cGAS knockout significantly downregulated the abundance of interferon and other cytokines induced by Ag85A-S2. In sum, Ag85-S2-mediated enhancement of immune responses was dependent on both cGAS and STING. Our results provided a new strategy for preventing Brucellosis from livestock, which might reduce the dosage and potential toxicity compared to the traditional S2 vaccine.