Ontology highlight
ABSTRACT:
PROVIDER: PRJNA503117 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Welwitschia mirabilis Organism overview
| PRJNA311094 | ENA
Project description:Welwitschia mirabilis Genome sequencing and assembly
| PRJNA693632 | ENA
Project description:The aim of this experiment is to test the ability of the ortholog of Arabidopsis LFY gene from flowering and non flowering species to complement an Arabidopsis LFY mutant. <br>Plants expressing, in a homozygous lfy12 background, the open reading frame of LFY orthologs under the control of Arabidopsis LFY promoter were synchronously induced to flower by growing plants in short days for 30 days then shifting them to Long Day for an additional 8 days. Shoot apices were dissected at either d0 or d8 in long days. Two biological replicates were performed. The following genotypes were used: <br>Col - wild type arabidopsis; reference strain<br>LFY (lfy12; LFY::AthLFY) - Arabidopsis<br>UNI (lfy12; LFY::UNI) - Pisum<br>ALF (lfy12; LFY::ALF) - Petunia <br>WelNDLY (lfy12; LFY::WelNDLY) - Welwitschia mirabilis<br>CrLFY1 (lfy12; LFY::CrLFY1) - Ceratopteris richardii<br>CrLFY2 (lfy12; LFY::CrLFY2) - Ceratopteris richardii<br>lfy-2; weak lfy allele<br>lfy-9; intermediate leafy allele<br>lfy-12; strong leafy allele.
2009-07-23 | E-MEXP-2258 | biostudies-arrayexpress
Project description:Proteus mirabilis is a leading cause of catheter-associated urinary tract infections (UTIs) and urolithiasis. The transcriptional regulator MrpJ inversely modulates two critical aspects of P. mirabilis UTI progression: fimbria-mediated attachment to the urinary tract, and flagella-mediated motility. Chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) was used for the first time in a CAUTI pathogen to probe for in vivo direct targets of MrpJ. ChIP-seq revealed 81 78 direct MrpJ targets, including genes for motility, fimbriae and a type VI secretion system (T6SS), and the putative MrpJ binding sequence ACnCnnnnnnnGnGT.
2018-08-10 | GSE96042 | GEO
Project description:Proteus mirabilis is a primary cause of complicated urinary tract infections (UTI). Surprisingly, iron acquisition systems have been poorly characterized in this uropathogen despite the urinary tract being iron-limited. In this report the transcriptome of strain HI4320, cultured under iron limitation, was examined using microarray analysis. Of genes upregulated at least 2-fold, 45 were statistically significant and comprise 21 putative iron-regulated systems. Two of these systems, PMI0229-0239 and PMI2596-2605, are organized in operons and appear to encode siderophore biosynthesis genes. Five microarrays comparing P. mirabilis HI4320 cultured in LB broth to P. mirabilis cultured in LB broth + 15 uM Desferal (an iron chelator) were analyzed. All five arrays are biological replicates; arrays #2 and 4 are dye swaps.
2010-09-09 | E-GEOD-18051 | biostudies-arrayexpress
Project description:The goal of this study was to measure the effect of heat stress on the transcriptome of a temperate fish species - Gillichthys mirabilis. Keywords: Stress response
2007-12-14 | GSE9870 | GEO
Project description:The goal of this study was to measure the effect of heat stress on the transcriptome of a cold-adapted fish (Trematomus bernacchii) and compare it to a temperate species (Gillichthys mirabilis). This SuperSeries is composed of the SubSeries listed below.
2007-12-14 | GSE9872 | GEO
Project description:The enteric bacterium Proteus mirabilis is a common cause of complicated urinary tract infections. In the study, microrarrays were used to analyze P. mirabilis gene expression in vivo from experimentally infected mice. Urine was collected at 1, 3, and 7d postinfection, and RNA was isolated from bacteria in the urine for transcriptional analysis. Across 9 microarrays, 471 genes were upregulated and 82 were downregulated in vivo compared to in vitro broth culture. Genes upregulated in vivo encoded MR/P fimbriae, urease, iron uptake systems, amino acid and peptide transporters, pyruvate metabolism, and portions of the TCA cycle. Flagella were downregulated. Ammonia assimilation gene glnA (glutamine synthetase) was repressed in vivo while gdhA (glutamate dehydrogenase) was upregulated in vivo. Contrary to our expectations, ammonia availability due to urease activity in P. mirabilis did not drive this gene expression. A gdhA mutant was growth-deficient in minimal medium with citrate as the sole carbon source, and loss of gdhA resulted in a significant fitness defect in the mouse model of urinary tract infection. Unlike Escherichia coli, which represses gdhA and upregulates glnA in vivo and cannot utilize citrate, the data suggest that P. mirabilis uses glutamate dehydrogenase to monitor carbon-nitrogen balance, and this ability contributes to the pathogenic potential of P. mirabilis in the urinary tract.
2011-11-29 | GSE25977 | GEO