Project description:To investigate the function of Opa1 in the regulation of adult neurogenesis, we created a conditional Opa1-knockout mouse line by cross-breeding Nestin-CreERT2;ROSA26YFP mice with Opa1-Flox mice. Using Tamoxifen, we induced Opa1 knockout on 8-weeks-old Nestin-CreERT2;ROSA26YFP;Opa1-Flox mice. We then sorted YFP-positive cells from the hippocampus using flow cytometry at 10 weeks. RNA was extracted from sorted cells and subjected to next generation sequencing.

2024-06-17 | GSE233464 | GEO

RNA sequencing of LINC00958 silenced and LINC00958 non-silenced Ishikawa cells

Project description:To confirm the downstream genes of LINC00958, we employed RNA sequencing (RNA-seq) to identify the differentially expressed genes when LINC00958 was silenced in Ishikawa cells. The sample of LINC00958-silenced Ishikawa cells (sh-LINC00958, n=3) and negative control cells (sh-NC, n=2) were sent to Shanghai Sangon Biotech of China for library construction, sequencing, data preprocessing and gene mapping. Sequencing was performed on an DNBSEQ-T7 platform for the generation of raw data. Genes were considered significantly differentially expressed if the P value < 0.05 and |log2FC| > 1.The results showed that a total of 305 mRNAs were regulated after LINC00958 knockdown (fold change>2, P<0.05)

2022-03-16 | GSE198619 | GEO

small RNA sequencing of human endothelial cells (HUVECs)

Project description:small RNA sequencing of human endothelial cells (HUVECs)

| PRJNA414622 | ENA

RNA sequencing for HUVECs treated with si-FLRT2

Project description:RNA sequencing for HUVECs treated with si-FLRT2

| PRJNA745197 | ENA

Transcriptome sequencing of HUVECs

Project description:Transcriptome sequencing of HUVECs

| PRJNA649251 | ENA

Gene expression data in control (shCtrl) and B55alpha-silenced (shB55a KD) HUVECs

Project description:Gene expression data in control (shCtrl) and B55alpha-silenced (shB55a KD) HUVECs