Project description:To investigate the molecular mechanisms of cardiac glycosides’ neuroprotection, we profiled transcriptomes of human iPSC-neurons after 72h of treatment with increasing doses of digoxin, oleandrin, proscillaridin A or vehicle alone (0.1% DMSO) using RNA sequencing.
Project description:IMR90 ER:RAS cells were treated with 4OHT to induce senescence. 6 days later they were treated with Vehicle, Ouabain or Digoxin for 16 or 36h and collected he cells were collected for total mRNA analysis. Minus (Cell not treated with 4OHT), Plus (Cell treated with 4OHT and vehicle), Plus C (On to pof the aforementiones treatments, cells were treated with Caspase inhibitors O/N)
Project description:Normal human cardiac fibroblast were treated with 200 nM halofuginone for 24 hours and the transcriptomic profile was measured by RNAseq.
Project description:Prostate cancer is the most common cancer in men and cardiac glycosides inhibit prostate cancer cell proliferation. In order to investigate the mechanism by which cardiac glycosides inhibit prostate cancer cells, we observed genome-wide RNA expression in prostate cancer LNCaP-abl cells, hormone resistant cells, after the cardiac glycoside treatment using RNA-Seq. In addition, we profiled LNCaP-abl cells after androgen receptor (AR) knockdown to observe whether cardiac glycoside effect on RNA expression is similar to that of AR knockdown. Observation of three cardioglycosides, Digoxin, Peruvoside and Strophanthidin, and AR knockdown regulated RNA expression in LNCaP-abl with RNA-Seq (each triplicates)
Project description:Quantitative analysis of global proteomic changes in proliferating and senescent BJ fibroblasts, treated with eIF5A hypusination inhibitor GC7 (10 uM) for 12h.