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Effects of Cardiac Glycosides on RNA Expression in Prostate Cancer LNCaP-abl Cells

ABSTRACT: Prostate cancer is the most common cancer in men and cardiac glycosides inhibit prostate cancer cell proliferation. In order to investigate the mechanism by which cardiac glycosides inhibit prostate cancer cells, we observed genome-wide RNA expression in prostate cancer LNCaP-abl cells, hormone resistant cells, after the cardiac glycoside treatment using RNA-Seq. In addition, we profiled LNCaP-abl cells after androgen receptor (AR) knockdown to observe whether cardiac glycoside effect on RNA expression is similar to that of AR knockdown. Observation of three cardioglycosides, Digoxin, Peruvoside and Strophanthidin, and AR knockdown regulated RNA expression in LNCaP-abl with RNA-Seq (each triplicates)

ORGANISM(S): Homo sapiens

SUBMITTER: Hai-Ri Li

PROVIDER: E-GEOD-35126 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Versatile pathway-centric approach based on high-throughput sequencing to anticancer drug discovery.

Li Hairi H Zhou Hongyan H Wang Dong D Qiu Jinsong J Zhou Yu Y Li Xiangqiang X Rosenfeld Michael G MG Ding Sheng S Fu Xiang-Dong XD

Proceedings of the National Academy of Sciences of the United States of America 20120306 12

The advent of powerful genomics technologies has uncovered many fundamental aspects of biology, including the mechanisms of cancer; however, it has not been appropriately matched by the development of global approaches to discover new medicines against human diseases. Here we describe a unique high-throughput screening strategy by high-throughput sequencing, referred to as HTS(2), to meet this challenge. This technology enables large-scale and quantitative analysis of gene matrices associated wi ...[more]

PMID: 22396588

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