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Scyliorhinus canicula

ABSTRACT: Scyliorhinus canicula Transcriptome or Gene expression

PROVIDER: PRJNA504730 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8179290_1.fastq.gz	Fastqsanger.gz
	SRR8179290_2.fastq.gz	Fastqsanger.gz
	SRR8179291_1.fastq.gz	Fastqsanger.gz
	SRR8179291_2.fastq.gz	Fastqsanger.gz
	SRR8179289_1.fastq.gz	Fastqsanger.gz

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Project description:The retina, whose basic cellular structure is highly conserved across vertebrates, constitutes an accessible system for studying the central nervous system. In recent years, single-cell RNA-sequencing studies have uncovered cellular diversity in the retina of a variety of species, providing new insights on retinal evolution and development. However, similar data in cartilaginous fishes, the sister group to all other extant jawed vertebrates, are still lacking. Here, we present a single-nucleus RNA-sequencing atlas of the postnatal retina of the catshark Scyliorhinus canicula, consisting of the expression profiles for 17,438 individual cells from three female, juvenile catshark specimens. Unsupervised clustering revealed 22 distinct cell types comprising all major retinal cell classes, as well as retinal progenitor cells (whose presence reflects the persistence of proliferative activity in postnatal stages in sharks) and oligodendrocytes. Thus, our dataset serves as a foundation for further studies on the development and function of the catshark retina. Moreover, integration of our atlas with data from other species will allow for a better understanding of vertebrate retinal evolution.

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Project description:Genome sequencing of the catshark Scyliorhinus canicula

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Late-stage embryonic gene expression in three cartilaginous fishes

Project description:Gene expression profiling of pooled late stage embryos from Leucoraja erinacea, Scyliorhinus canicula and Callorhinchus milii show that HOXC cluster genes are not expressed in the two elasmobranch fishes, L. erinacea and S. canicula. This finding supports the observations that these genes are not found in whole genome shotgun sequencing of L. erinacea or genomic clones from S. canicula.

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Late-stage embryonic gene expression in three cartilaginous fishes

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