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Expression of OsTPX Gene Improves Cellular Redox Homeostasis and Photosynthesis Efficiency in Synechococcus elongatus PCC 7942

ABSTRACT: Expression of OsTPX Gene Improves Cellular Redox Homeostasis and Photosynthesis Efficiency in Synechococcus elongatus PCC 7942

PROVIDER: PRJNA506580 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8237705_1.fastq.gz	Fastqsanger.gz
	SRR8237705_2.fastq.gz	Fastqsanger.gz
	SRR8237706_1.fastq.gz	Fastqsanger.gz
	SRR8237706_2.fastq.gz	Fastqsanger.gz
	SRR8237707_1.fastq.gz	Fastqsanger.gz

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Expression of OsTPX Gene Improves Cellular Redox Homeostasis and Photosynthesis Efficiency in Synechococcus elongatus PCC 7942

Project description:The goals of this study are to compare hydrogen peroxide (H2O2) stress tolerance through gene expression of wild type (WT) and OsTPX-expressing Synechoccous elongatus PCC 7942 (OT) under the normal and stress condition.

2018-12-26 | GSE122841 | GEO

Expression of Heterologous OsDHAR Gene Improves Glutathione (GSH)-Dependent Antioxidant System and Maintenance of Cellular Redox Status in Synechococcus elongatus PCC 7942

Project description:An excess of reactive oxygen species (ROS) can cause severe oxidative damage to cellular components in photosynthetic cells. Antioxidant systems, such as the ascorbate-glutathione cycle, regulate redox status in cells to guard against such damage. Dehydroascorbate reductase (DHAR, EC 1.8.5.1) catalyzes the glutathione-dependent reduction of oxidized ascorbate (dehydroascorbate) and contains a redox active site and glutathione binding-site. The DHAR gene is important in biological and abiotic stress responses involving reduction of the oxidative damage caused by ROS. In this study, a transgenic microalgae (TA) strain was constructed by cloning the Oryza sativa L. japonica DHAR (OsDHAR) gene controlled by an isopropyl β-D-1-thiogalactopyranoside (IPTG)-inducible promoter (Ptrc) into the Synechococcus elongatus PCC 7942 strain of cyanobacteria to study the functional activities of OsDHAR under oxidative stress caused by hydrogen peroxide exposure. OsDHAR expression increased the growth of S. elongatus PCC 7942 under oxidative stress by reducing the levels of hydroperoxides and malondialdehyde (MDA) and mitigating the loss of chlorophyll. DHAR and glutathione S-transferase activity were higher than in the wild-type (WT) strain. Additionally, overexpression of OsDHAR in S. elongatus PCC 7942 greatly increased the glutathione (GSH)/glutathione disulfide (GSSG) ratio compared to ascorbic acid (AsA)/dehydroascorbate (DHA) ratio in the presence or absence of hydrogen peroxide. These results strongly suggest that DHAR attenuates deleterious oxidative effects via the glutathione (GSH)-dependent antioxidant system in cyanobacterial cells. The expression of heterologous OsDHAR in S. elongatus PCC 7942 protected cells from oxidative damage through a GSH-dependent antioxidant system via GSH-dependent reactions at the redox active site and GSH binding site residues during oxidative stress.

2019-11-25 | GSE140121 | GEO

Transcriptome analysis of Synechococcus elongatus PCC 7942 for nitrogen starvation response using RNA sequencing

Project description:We reported transcriptome analysis of S. elongatus PCC 7942 for nitrogen starvation response by performing RNA-sequencing. As a result, we identified differentially express genes and confirmed physiological response changes for up or down regulation genes. among differentially expressed genes (p value < 0.01) during N starvation

2017-02-14 | GSE79726 | GEO

Expression of OsTPX Gene Improves Cellular Redox Homeostasis and Photosynthesis Efficiency in Synechococcus elongatus PCC 7942.

Project description:Cyanobacterial 2-Cys peroxiredoxin (thioredoxin peroxidase, TPX) comprises a family of thiol antioxidant enzymes critically involved in cell survival under oxidative stress. In our previous study, a putative TPX was identified using a proteomics analysis of rice (Oryza sativa L. japonica, OsTPX) seedlings exposed to oxidative stress. This OsTPX gene is structurally similar to the Synechococcus elongatus TPX gene in the highly conserved redox-active disulfide bridge (Cys114, Cys236) and other highly conserved regions. In the present study, the OsTPX gene was cloned into rice plants and S. elongatus PCC 7942 strain to study hydrogen peroxide (H2O2) stress responses. The OsTPX gene expression was confirmed using semi-quantitative RT-PCR and western blot analysis. The OsTPX gene expression increased growth under oxidative stress by decreasing reactive oxygen species and malondialdehyde level. Additionally, the OsTPX gene expression in S. elongatus PCC 7942 (OT) strain exhibited a reduced loss of chlorophyll and enhanced photosynthesis efficiency under H2O2 stress, thereby increasing biomass yields twofold compared with that of the control wild type (WT) strain. Furthermore, redox balance, ion homeostasis, molecular chaperone, and photosynthetic systems showed upregulation of some genes in the OT strain than in the WT strain by RNA-Seq analysis. Thus, OsTPX gene expression enhances oxidative stress tolerance by increasing cell defense regulatory networks through the cellular redox homeostasis in the rice plants and S. elongatus PCC 7942.

| S-EPMC6297720 | biostudies-literature

Improved salt tolerance of Synechococcus elongatus PCC 7942 by heterologous synthesis of compatible solute ectoine

Project description:In order to explore the effect of introducing the ectoine synthesis module in Synechococcus elongatus PCC 7942 on the global regulation of the strain under high-salt environment. The ectoine synthesis module was introduced at the Synpcc7942_0808 locus to obtain the Syn7942/Δsps-ect strain that lacked sucrose synthesis and could synthesize ectoine. Comparative transcriptomic analysis was performed on WT and Syn7942/Δsps-ect under 0 mM and 300 mM NaCl conditions.

2023-01-08 | GSE222067 | GEO

Synechococcus elongatus PCC 7942 = FACHB-805

Project description:Synechococcus elongatus PCC 7942 = FACHB-805 Genome sequencing

| PRJNA268360 | ENA

Synechococcus elongatus PCC 7942 = FACHB-805

Project description:Synechococcus elongatus PCC 7942 = FACHB-805 Raw sequence reads

| PRJNA1026715 | ENA

Synechococcus elongatus PCC 7942 = FACHB-805

Project description:Synechococcus elongatus PCC 7942 = FACHB-805 Raw sequence reads

| PRJNA933362 | ENA

Synechococcus elongatus PCC 7942 = FACHB-805

Project description:Synechococcus elongatus PCC 7942 = FACHB-805 Raw sequence reads