Project description:We have previously showed a strong genetic determinant governing resistance of the M. truncatula A17 line to C. trifolii is located in a chromosomal region at the top of chromosome 4 (Ameline-torregrosa et al., 2008). This region also contains the RCT1 gene which has been shown to confer resistance to C. trifolii when transferred to a susceptible alfalfa plants (Zhu et al.), In order to evaluate the role of this region in the response to non-adapted Colletorichum species and to compare this response to those induce by C. trifolii, two M. truncatula near-isogenic lines differing only in this chromosomal region were used for transcript profiling experiments. These two lines were issued from a recombinant-inbred collection obtained from a A17-F83005.5 cross (Cazaux et al., in preparation). Plant inoculations were done on two-week-old entire plants by spraying a conidial suspension of C. trifolii or C. lindemuthianum to avoid possible artifacts which can be observed on detached leaves (Liu et al., 2007). RNA was extracted from leaves collected at 1dpi and 3dpi and transcript profiling was performed using Mt16kOLI1Plus chips (Thompson et al., 2005).
Project description:We analyzed mK562 cells with miR-543 overexpressed or empty vector to determine the mRNA profile of miR-543 overexpressed K562 cells. We used Affymetrix Clariom S Human Array to profile the differentially expressed mRNA in miR-543 overexpressed and control cells
