Project description:To identify BRAT1 target loci at the whole-genome level, we performed RNA deep sequencing and examined the transcript levels of transposable elements and genes in the wild type, brat1, nrpe1, and brat1nrpe1. Compare the mRNA profiles of 10-day old seedlings materials of mutants (brat1, nrpe1, and brat1nrpe1) to wild type by Illumina suquencing.
Project description:Primary objectives: The primary objective is to investigate circulating tumor DNA (ctDNA) via deep sequencing for mutation detection and by whole genome sequencing for copy number analyses before start (baseline) with regorafenib and at defined time points during administration of regorafenib for treatment efficacy in colorectal cancer patients in terms of overall survival (OS).
Primary endpoints: circulating tumor DNA (ctDNA) via deep sequencing for mutation detection and by whole genome sequencing for copy number analyses before start (baseline) with regorafenib and at defined time points during administration of regorafenib for treatment efficacy in colorectal cancer patients in terms of overall survival (OS).
Project description:To study how MMS19 and ABO4 affect gene expression, we performed RNA deep sequencing for the seedlings materials Col, mms19-1,and abo4 mutants. Compare the mRNA profiles of 14-day old seedlings materials of mutants (mms19-1 and abo4) to wild type Col by Illumina suquencing.
Project description:To study how PRP31, ZOP1 and STA1 affect gene expression and pre-mRNA splicing, we performed RNA deep sequencing for the seedlings materials C24, prp31, zop1, and sta1 mutants. Compare the mRNA profiles of 10-day old seedlings materials of mutants (prp31, zop1 and sta1) to wild type C24 by Illumina suquencing.
Project description:To check the dMyc function, RNA profiling was achieved by the RNA-seq assay comparing mRNA levels of lst81, dm0, lst81dm0 and rictorΔ1 in the adult heads of male mutant animals with wild-type controls Compare the mRNA profiles of 5-day old adult head materials of mutants (lst81, dmP0, lst81dmP0 and rictor1) to wild type W1118 by Illumina suquencing.