Project description:To identify BRAT1 target loci at the whole-genome level, we performed RNA deep sequencing and examined the transcript levels of transposable elements and genes in the wild type, brat1, nrpe1, and brat1nrpe1. Compare the mRNA profiles of 10-day old seedlings materials of mutants (brat1, nrpe1, and brat1nrpe1) to wild type by Illumina suquencing.
Project description:Primary objectives: The primary objective is to investigate circulating tumor DNA (ctDNA) via deep sequencing for mutation detection and by whole genome sequencing for copy number analyses before start (baseline) with regorafenib and at defined time points during administration of regorafenib for treatment efficacy in colorectal cancer patients in terms of overall survival (OS).
Primary endpoints: circulating tumor DNA (ctDNA) via deep sequencing for mutation detection and by whole genome sequencing for copy number analyses before start (baseline) with regorafenib and at defined time points during administration of regorafenib for treatment efficacy in colorectal cancer patients in terms of overall survival (OS).
Project description:To study how MMS19 and ABO4 affect gene expression, we performed RNA deep sequencing for the seedlings materials Col, mms19-1,and abo4 mutants. Compare the mRNA profiles of 14-day old seedlings materials of mutants (mms19-1 and abo4) to wild type Col by Illumina suquencing.
Project description:To study how PRP31, ZOP1 and STA1 affect gene expression and pre-mRNA splicing, we performed RNA deep sequencing for the seedlings materials C24, prp31, zop1, and sta1 mutants. Compare the mRNA profiles of 10-day old seedlings materials of mutants (prp31, zop1 and sta1) to wild type C24 by Illumina suquencing.
Project description:To check the dMyc function, RNA profiling was achieved by the RNA-seq assay comparing mRNA levels of lst81, dm0, lst81dm0 and rictorΔ1 in the adult heads of male mutant animals with wild-type controls Compare the mRNA profiles of 5-day old adult head materials of mutants (lst81, dmP0, lst81dmP0 and rictor1) to wild type W1118 by Illumina suquencing.
Project description:Chronic epididymitis (CE) refers to a long-lasting inflammatory condition of the epididymis, which is considered the most common site of intrascrotal inflammation and an important aetiological factor of male infertility. Recent studies demonstrate that small RNAs secreted from epididymal epithelium modulate embryo development and offspring phenotypes via sperm transmission, and the resulting modifications may lead to transgenerational inheritance. However, to date, the genome-wide analysis of small RNA together with the transcriptomic expression profiles of human epididymis and CE is still lacking. In this study, we collect inflamated epdidymis from patients as well as control sample. By simultaneous mRNA and small RNA suquencing, we identified segment-specific inflammation signitures including leukocyte chemotaxis, ion channel and transporter-related processes. We also compare the miRNA, tsRNA, and other small RNA distribution in CE. Together, Interative analysis of miRNA and mRNA identified strong candidate epigenitic cycle in CE.