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Hordeum vulgare cultivar:Golden Promise

ABSTRACT: BTH treatment on barley NPR1 transgenic lines Transcriptome

PROVIDER: PRJNA509975 | ENA |

REPOSITORIES: ENA

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Hordeum vulgare cultivar:Golden Promise

Project description:Barley NPR1-mediated acquired resistance RNA-seq

| PRJNA431836 | ENA

Transcriptomic changes in the crown (stem base) of barley (Hordeum vulgare L. cv. Golden Promise) during crown-root initiation and development

Project description:Purpose: The goal of this study was to investigate the mechanisms involved in the initiation and development of crown-roots (CRs) in barley and to estimate the role of cytokinins (CKs) in this process. Method: stranded libraries were obtained from RNA extracted from the stem base of 1 day-after-germination (DAG) and 10DAG-seedlings of wild-type (WT) and AtCKX-overexpressing barley lines (OE-CKX). OE-CKX lines have a reduced content of endogenous CKs and are characterized by a higher number of CRs. Libraries were deep sequenced on Illumina HighSeq platform. Each sample was investigated in three independent biological replicates. Results: Using a data analysis workflow optimized for barley, we identified more than 4000 transcripts differentially expressed in the stem base of 1DAG and 10DAG-seedlings. Expression as determined by RNA-seq was validated by real-time PCR. Our data were compared to the transcriptomic profiling obtained from rice and we were able to identify genes potentially involved in the initiation/development of CRs in barley. Also the use of the transgenic line with altered endogenous CK content allowed us to conclude about the role of CKs in the process. Conclusions: Our study represents the first analysis aiming to understand the initiation and development of CRs in barley.

2016-11-01 | GSE87737 | GEO

Hordeum vulgare cultivar:Golden Promise

Project description:MADS1 maintains barley spike determinacy at high ambient temperatures

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Hordeum vulgare cultivar:Golden Promise

Project description:Screening-by sequencing of a barley Golden Promise TILLING population

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Hordeum vulgare subsp. vulgare cultivar:Golden Promise

Project description:Sequence capture, sequencing, and assembly of NLR gene space in barley accession Golden Promise

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Hordeum vulgare

Project description:Substantial equivalence in transgenic barley

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RNA sequencing of Hordeum vulgare near isogenic lines

Project description:NILs containing five parental lines, three wild barley genotypes ssp. spontaneum: HID 4 (A), Iraq; HID 64 (B), Turkey; and HID 369 (C), Israel, one ssp. agriocrithon: HID 382(D)) and cv. Morex (ssp. vulgare, USA). Purpose: Variant calling to identifie markers associated with a awn length QTL on the distal part of chromosome 7HL

2017-03-08 | GSE85338 | GEO

Hordeum vulgare cultivar:Golden Promise

Project description:Hordeum vulgare cultivar:Golden Promise Raw sequence reads

| PRJNA612176 | ENA

Hordeum vulgare cultivar:Golden Promise

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| PRJNA593943 | ENA

Hordeum vulgare strain:Golden Promise

Project description:Hordeum vulgare Genome sequencing

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