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Epithelial Vegfa specifies a distinct endothelial population in the mouse lung [bulk RNA-seq]

ABSTRACT: Epithelial Vegfa specifies a distinct endothelial population in the mouse lung [bulk RNA-seq]

PROVIDER: PRJNA511608 | ENA |

REPOSITORIES: ENA

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Epithelial Vegfa specifies a distinct endothelial population in the mouse lung [bulk RNA-seq]

Project description:The lung microvasculature is essential for gas exchange and commonly considered homogeneous. We show that Vascular endothelial growth factor A (Vegfa) from the epithelium specifies a distinct endothelial cell (EC) population in the postnatal mouse lung. Vegfa is predominantly expressed by alveolar type 1 (AT1) cells and locally required to specify a subset of ECs. Single cell RNA-seq identified 15~20% lung ECs as transcriptionally distinct and marked by Carbonic anhydrase 4 (Car4), which are specifically lost upon epithelial Vegfa deletion. Car4 ECs, unlike bulk ECs, have extensive cellular projections and are separated from AT1 cells by a limited basement membrane without any intervening pericytes. Without Car4 ECs, the alveolar space is aberrantly enlarged despite the normal appearance of myofibroblasts. Lung Car4 ECs and retina tip ECs have common and distinct transcriptional profiles. These findings support a signaling role of AT1 cells and shed light on alveologenesis.

2020-01-01 | GSE124324 | GEO

Epithelial Vegfa specifies a distinct endothelial population in the mouse lung [scRNA-seq]

2020-01-01 | GSE124323 | GEO

Epithelial Vegfa specifies a distinct endothelial population in the mouse lung

Project description:Epithelial Vegfa specifies a distinct endothelial population in the mouse lung

| PRJNA511606 | ENA

Epithelial Vegfa specifies a distinct endothelial population in the mouse lung [scRNA-seq]

Project description:Epithelial Vegfa specifies a distinct endothelial population in the mouse lung [scRNA-seq]

| PRJNA511607 | ENA

Epithelial Vegfa Specifies a Distinct Endothelial Population in the Mouse Lung.

Project description:The lung microvasculature is essential for gas exchange and commonly considered homogeneous. We show that VEGFA from the epithelium is required for a distinct endothelial cell (EC) population in the mouse lung. Vegfa is predominantly expressed by alveolar type 1 (AT1) cells and locally required to specify a subset of ECs. Single-cell RNA sequencing (scRNA-seq) reveals that ∼15% of lung ECs are transcriptionally distinct-marked by Carbonic anhydrase 4 (Car4)-and arise from bulk ECs, as suggested by trajectory analysis. Car4 ECs have extensive cellular projections and are separated from AT1 cells by a limited basement membrane without intervening pericytes. Car4 ECs are specifically lost upon epithelial Vegfa deletion; without Car4 ECs, the alveolar space is aberrantly enlarged despite the normal appearance of myofibroblasts. Lung Car4 ECs and retina tip ECs have common and distinct features. These findings support a signaling role of AT1 cells and shed light on alveologenesis.

| S-EPMC7170573 | biostudies-literature

Epithelial Vegfa specifies a distinct endothelial population in the mouse lung

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-01-01 | GSE124325 | GEO

Two faces of bivalent domain regulate VEGFA responsiveness and angiogenesis

Project description:The bivalent domain at promoter region is a unique epigenetic feature poised for activation or repression during cell differentiation in embryonic stem cell. However, the function of bivalent domains in already differentiated cells remains exclusive. By profiling the epigenetic landscape of endothelial cells during VEGFA stimulation, we discovered that bivalent domains are widespread in endothelial cells and preferentially marked genes responsive to VEGFA. The bivalent domains responsive to VEGFA have more permissive chromatin environment comparing to other bivalent domains. The initial activation of bivalent genes depends on RNAPII pausing release induced by EZH1 rather than removal of H3K27me3. The later suppression of bivalent gene expression depended on KDM5A recruitment by its interaction with PRC2. Importantly, EZH1 promoted both in vitro and in vivo angiogenesis by upregulating EGR3, whereas KDM5A dampened angiogenesis. Collectively, this study demonstrated a novel dual function of bivalent domains in endothelial cells to control VEGF responsiveness and angiogenesis.

2020-10-17 | GSE139077 | GEO

Bulk RNA-sequencing of VE-Cadherin+/CD45- mouse aorta endothelial cells

Project description:In mouse aorta endothelial cells, populations of endothelial vascular progenitor (EVP) and differentiated (D) cells could be identified by CD31 (lo/hi) and VEGFR2 (lo/hi) expression. These populations were FACS sorted and paired-end bulk RNA-sequencing was performed.

2019-05-27 | E-MTAB-7148 | biostudies-arrayexpress

Methylation specifies distinct estrogen-induced binding site repertoires of CBP to chromatin

Project description:This SuperSeries is composed of the following subset Series: GSE32348: Methylation specifies distinct estrogen-induced binding site repertoires of CBP to chromatin (mRNA) GSE32349: Methylation specifies distinct estrogen-induced binding site repertoires of CBP to chromatin (ChIP-Seq) Refer to individual Series

2011-10-06 | E-GEOD-32350 | biostudies-arrayexpress

Bulk RNA-sequencing of FACS isolated human fetal lung, intestine, and kidney endothelial cells

Project description:Adult endothelial cells (ECs) are known to possess organ-specific gene expression, morphology and function, but whether organ-specific EC gene expression is present during human development is not known. Here, we used bulk RNA-sequencing (RNA-seq) to interrogate the developing human intestine, lung, and kidney in order to identify organ-enriched EC-gene signatures. FACS was used to isolate EC (CD31+CD144+, n=13) and non-EC (CD31-CD144-, n=16) populations from these three organs, profiling at least 4 biological replicates for each organ system. The biological specimens profiled were between 11-20 gestational weeks. We also sequenced cultured human umbilical vein endothelial cells (HUVECs) via bulk RNAseq. Computational approaches were used to identify organ-specific EC-enriched gene signatures across human fetal lung, intestine, and kidney ECs.

2020-12-20 | E-MTAB-9372 | biostudies-arrayexpress

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