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Epithelial Vegfa specifies a distinct endothelial population in the mouse lung

ABSTRACT: Epithelial Vegfa specifies a distinct endothelial population in the mouse lung

PROVIDER: PRJNA511606 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR8367590_1.fastq.gz	Fastqsanger.gz
	SRR8367590_2.fastq.gz	Fastqsanger.gz
	SRR8367581_1.fastq.gz	Fastqsanger.gz
	SRR8367581_2.fastq.gz	Fastqsanger.gz

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Epithelial Vegfa specifies a distinct endothelial population in the mouse lung [scRNA-seq]

Project description:The lung microvasculature is essential for gas exchange and commonly considered homogeneous. We show that Vascular endothelial growth factor A (Vegfa) from the epithelium specifies a distinct endothelial cell (EC) population in the postnatal mouse lung. Vegfa is predominantly expressed by alveolar type 1 (AT1) cells and locally required to specify a subset of ECs. Single cell RNA-seq identified 15~20% lung ECs as transcriptionally distinct and marked by Carbonic anhydrase 4 (Car4), which are specifically lost upon epithelial Vegfa deletion. Car4 ECs, unlike bulk ECs, have extensive cellular projections and are separated from AT1 cells by a limited basement membrane without any intervening pericytes. Without Car4 ECs, the alveolar space is aberrantly enlarged despite the normal appearance of myofibroblasts. Lung Car4 ECs and retina tip ECs have common and distinct transcriptional profiles. These findings support a signaling role of AT1 cells and shed light on alveologenesis.

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Epithelial Vegfa specifies a distinct endothelial population in the mouse lung [bulk RNA-seq]

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Epithelial Vegfa specifies a distinct endothelial population in the mouse lung [scRNA-seq]

Project description:Epithelial Vegfa specifies a distinct endothelial population in the mouse lung [scRNA-seq]

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Epithelial Vegfa specifies a distinct endothelial population in the mouse lung [bulk RNA-seq]

Project description:Epithelial Vegfa specifies a distinct endothelial population in the mouse lung [bulk RNA-seq]

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Epithelial Vegfa Specifies a Distinct Endothelial Population in the Mouse Lung.

Project description:The lung microvasculature is essential for gas exchange and commonly considered homogeneous. We show that VEGFA from the epithelium is required for a distinct endothelial cell (EC) population in the mouse lung. Vegfa is predominantly expressed by alveolar type 1 (AT1) cells and locally required to specify a subset of ECs. Single-cell RNA sequencing (scRNA-seq) reveals that ∼15% of lung ECs are transcriptionally distinct-marked by Carbonic anhydrase 4 (Car4)-and arise from bulk ECs, as suggested by trajectory analysis. Car4 ECs have extensive cellular projections and are separated from AT1 cells by a limited basement membrane without intervening pericytes. Car4 ECs are specifically lost upon epithelial Vegfa deletion; without Car4 ECs, the alveolar space is aberrantly enlarged despite the normal appearance of myofibroblasts. Lung Car4 ECs and retina tip ECs have common and distinct features. These findings support a signaling role of AT1 cells and shed light on alveologenesis.

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Epithelial Vegfa specifies a distinct endothelial population in the mouse lung

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