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The landscape of mRNA translation in tomato roots

ABSTRACT: The landscape of mRNA translation in tomato roots

PROVIDER: PRJNA514710 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8434771_1.fastq.gz	Fastqsanger.gz
	SRR8434771_2.fastq.gz	Fastqsanger.gz
	SRR8434772_1.fastq.gz	Fastqsanger.gz
	SRR8434772_2.fastq.gz	Fastqsanger.gz
	SRR8434773_1.fastq.gz	Fastqsanger.gz

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The landscape of mRNA translation in tomato roots

Project description:We used Ribo-seq (Ribosome profiling) combining with RNA-seq to explore the translational landscape of tomato roots. We generated three biological replicates of RNA-seq and Ribo-seq data for tomato roots. We next used the RNA-seq result for de novo transcriptome assembly and Ribo-seq to identify novel translated open reading frames (ORFs). Our data revealed more than three hundreds of novel translated ORFs on previously unannotated transcripts. Most of the newly identified ORFs are small and difficult to detect with in silico methods. We also identified over thirteen hundreds of upstream ORFs on annotated genes. This data could facilitate gene annotation. Besides, this data also demonstrated that uORFs, miRNAs and antisense RNAs are regulating the expression of associated genes. This study uncovered mechanisms of translational regulation and gene annotation in tomato.

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