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A cancer stem cell hierarchy based on differential ribosomal RNA and protein synthesis capacities [EPHB2]

ABSTRACT: A cancer stem cell hierarchy based on differential ribosomal RNA and protein synthesis capacities [EPHB2]

PROVIDER: PRJNA515612 | ENA |

REPOSITORIES: ENA

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A cancer stem cell hierarchy based on differential ribosomal RNA and protein synthesis capacities [EPHB2]

Project description:Ribosomal RNA (rRNA) transcription and protein synthesis are heterogeneously regulated in colorectal tumors and are gradually shut down during cell differentiation. Cancer stem cell hierarchy and tumor growth is defined by a tumor cell population that displays elevated rRNA and protein synthesis.

2020-05-22 | GSE125229 | GEO

A cancer stem cell hierarchy based on differential ribosomal RNA and protein synthesis capacities

Project description:A cancer stem cell hierarchy based on differential ribosomal RNA and protein synthesis capacities

| PRJNA515608 | ENA

A cancer stem cell hierarchy based on differential ribosomal RNA and protein synthesis capacities [POLR1A]

2020-05-22 | GSE125231 | GEO

A cancer stem cell hierarchy based on differential ribosomal RNA and protein synthesis capacities [POLR1A]

Project description:A cancer stem cell hierarchy based on differential ribosomal RNA and protein synthesis capacities [POLR1A]

| PRJNA515613 | ENA

Zonation of ribosomal DNA transcription defines a stem cell hierarchy in colorectal cancer

Project description:Colorectal cancers (CRCs) are composed of an amalgam of cells with distinct genotypes and phenotypes. Here we reveal a previously unappreciated heterogeneity in the biosynthetic capacities of CRC cells. We discover that the majority of ribosomal DNA transcription and protein synthesis in CRCs occur in a limited subset of tumor cells that localize in defined niches. The rest of the tumor cells undergo an irreversible loss of their biosynthetic capacities as a consequence of differentiation. Cancer cells within the biosynthetic domains are characterized by elevated levels of the RNA Polymerase 1 subunit A - POLR1A. Genetic ablation of POLR1A-high cell population imposes an irreversible growth arrest to CRCs. We show that elevated biosynthesis defines stemness in both LGR5+ and LGR5- tumor cells. Therefore, a common architecture in CRC is a simple cell hierarchy based on the differential capacity to transcribe ribosomal DNA and synthesize proteins.

2020-04-09 | GSE148345 | GEO

A cancer stem cell hierarchy based on differential ribosomal RNA and protein synthesis capacities

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-05-22 | GSE125232 | GEO

EphB2 Receptor Promotes Dermal Fibrosis in Systemic Sclerosis

Project description:Objectives: Eph/Ephrin cell-cell signaling is emerging as a key player in tissue fibrogenesis. The aim of this study was to test the hypothesis that the receptor tyrosine kinase EphB2 mediates dermal fibrosis in systemic sclerosis (SSc). Methods: We assessed normal and SSc human skin biopsies for EphB2 expression. The in vivo role of EphB2 in skin fibrosis was investigated by subjecting EphB2-knockout mice to both bleomycin-induced and tight skin (Tsk1/+) genetic mouse models. EphB2 kinase-dead and overactive point mutant mice were used to evaluate the role of EphB2 forward signaling in bleomycin-induced dermal fibrosis. In vitro studies were performed on dermal fibroblasts from SSc patients and healthy controls, which was followed by in vivo analysis of fibroblast-specific EphB2 deficient mice. Results: Expression of EphB2 is upregulated in SSc skin tissue and explanted SSc dermal fibroblasts compared to healthy controls. EphB2 expression is elevated in two animal models of dermal fibrosis. In mice, EphB2 drives dermal fibrosis in both the bleomycin and the Tsk1/+ models of skin fibrosis. EphB2 forward signaling is a critical mediator of dermal fibrosis. Transforming growth factor-β (TGFβ) cytokines upregulate EphB2 in dermal fibroblasts via non-canonical TGFβ/SMAD signaling and silencing EphB2 in dermal fibroblasts is sufficient to dampen TGFβ-induced fibroblast-to-myofibroblast differentiation. Moreover, mice with fibroblast-specific deletion of EphB2 showed impaired fibroblast-to-myofibroblast differentiation and reduced skin fibrosis upon bleomycin challenge. Conclusion: Our data implicate EphB2 overexpression and kinase-mediated forward signaling in the development of dermal fibrosis in SSc. EphB2 thus represents a potential new therapeutic target for SSc.

2024-04-10 | GSE253089 | GEO

Transcriptional targets of EphB2 and Ephrin-A4 signaling in epidermal keratinocytes

Project description:Human epidermal keratinocytes were treated with 25 ng.ml EphB2 or EFNA4, both as-Fc conjugates (Sigma). Human epidermal keratinocytes are treated with 25 ng/ml EphB2 or EFNA4 Fc conjugates in a 48hr time course.

2011-03-01 | E-GEOD-26523 | biostudies-arrayexpress

Zonation of ribosomal DNA transcription defines a stem cell hierarchy in colorectal cancer

Project description:Zonation of ribosomal DNA transcription defines a stem cell hierarchy in colorectal cancer

| PRJNA623941 | ENA

Analysis of the gene expression profile in cutaneous squamous cell carcinoma cells after EphB2 knockdown

Project description:The role of Eph/ephrin signaling in numerous biological processes has been established. However, Eph/ephrin signaling has been shown to have complex role in tumor progression. The role of EphB2 receptor in the progression of cutaneous squamous cell carcinoma (cSCC) has not been studied before. EphB2 is significantly overexpressed in cSCC cells compared with normal human epidermal keratinocytes (NHEKs). We used microarray based global gene expression profiling to study the effect of EphB2 knockdown on the expression of different genes in cSCC cells. cSCC cell lines (n=3) were transfected with either control or EphB2 siRNA (75 nM) and incubated for 72 hours. Total RNA was extracted and processed expression profiling with GeneChip 3’ IVT Express Kit according to manufacturer’s protocol.

2015-08-27 | E-GEOD-66361 | biostudies-arrayexpress

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