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A cancer stem cell hierarchy based on differential ribosomal RNA and protein synthesis capacities [POLR1A]

ABSTRACT: A cancer stem cell hierarchy based on differential ribosomal RNA and protein synthesis capacities [POLR1A]

PROVIDER: PRJNA515613 | ENA |

REPOSITORIES: ENA

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A cancer stem cell hierarchy based on differential ribosomal RNA and protein synthesis capacities [POLR1A]

Project description:Ribosomal RNA (rRNA) transcription and protein synthesis are heterogeneously regulated in colorectal tumors and are gradually shut down during cell differentiation. Cancer stem cell hierarchy and tumor growth is defined by a tumor cell population that displays elevated rRNA and protein synthesis.

2020-05-22 | GSE125231 | GEO

A cancer stem cell hierarchy based on differential ribosomal RNA and protein synthesis capacities [EPHB2]

2020-05-22 | GSE125229 | GEO

A cancer stem cell hierarchy based on differential ribosomal RNA and protein synthesis capacities

Project description:A cancer stem cell hierarchy based on differential ribosomal RNA and protein synthesis capacities

| PRJNA515608 | ENA

A cancer stem cell hierarchy based on differential ribosomal RNA and protein synthesis capacities [EPHB2]

Project description:A cancer stem cell hierarchy based on differential ribosomal RNA and protein synthesis capacities [EPHB2]

| PRJNA515612 | ENA

Zonation of ribosomal DNA transcription defines a stem cell hierarchy in colorectal cancer

Project description:Colorectal cancers (CRCs) are composed of an amalgam of cells with distinct genotypes and phenotypes. Here we reveal a previously unappreciated heterogeneity in the biosynthetic capacities of CRC cells. We discover that the majority of ribosomal DNA transcription and protein synthesis in CRCs occur in a limited subset of tumor cells that localize in defined niches. The rest of the tumor cells undergo an irreversible loss of their biosynthetic capacities as a consequence of differentiation. Cancer cells within the biosynthetic domains are characterized by elevated levels of the RNA Polymerase 1 subunit A - POLR1A. Genetic ablation of POLR1A-high cell population imposes an irreversible growth arrest to CRCs. We show that elevated biosynthesis defines stemness in both LGR5+ and LGR5- tumor cells. Therefore, a common architecture in CRC is a simple cell hierarchy based on the differential capacity to transcribe ribosomal DNA and synthesize proteins.

2020-04-09 | GSE148345 | GEO

A cancer stem cell hierarchy based on differential ribosomal RNA and protein synthesis capacities

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-05-22 | GSE125232 | GEO

Zonation of ribosomal DNA transcription defines a stem cell hierarchy in colorectal cancer

Project description:Zonation of ribosomal DNA transcription defines a stem cell hierarchy in colorectal cancer

| PRJNA623941 | ENA

Cytoplasmic NAD+ Synthesis and Ribosomal Protein Mono(ADP-Ribosyl)ation Maintain Proteostasis in Ovarian Cancer

Project description:Global protein synthesis is upregulated in cancers, and thus cancer cells are prone to errors in translation, which may lead to protein misfolding and proteotoxic stress. The pathways that maintain protein homeostasis support the transformation and growth of cancer cells. Here, we show that the cytosolic NAD+ synthesis pathway (controlled by NMNAT-2) plays an essential role in ovarian cancer by maintaining protein homeostasis by regulating the activity of PARP-16, that MARylates the ribosomal proteins. Using polysome-RNA Seq analysis, we found that inhibiting ribosomal protein MARylation by knocking down NMNAT-2 or PARP-16 leads to increased association of specific mRNAs with polysomes and enhanced translation of a set of mRNAs that contain specific secondary structures in their 3’UTRs, which leads to protein aggregation. Collectively, our study demonstrates that NMNAT-2 regulates PARP-16 activity and ribosomal protein MARylation, which control protein homeostasis by fine-tuning the levels of protein synthesis in ovarian cancers.

2021-07-26 | GSE146458 | GEO

Regulated local synthesis of ribosomal proteins maintains ribosome function in axons

Project description:Regulated local synthesis of ribosomal proteins maintains ribosome function in axons

| PRJNA559103 | ENA

social hierarchy

Project description:social hierarchy Raw sequence reads

| PRJNA1108947 | ENA