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Modulation of TGF-B1 mediated fibroblast activation in human vocal fold fibroblast cells by Porcine-Derived Lamina Propria Extracellular Matrix Hydrogel

ABSTRACT: Modulation of TGF-B1 mediated fibroblast activation in human vocal fold fibroblast cells by Porcine-Derived Lamina Propria Extracellular Matrix Hydrogel

PROVIDER: PRJNA516437 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8472910_1.fastq.gz	Fastqsanger.gz
	SRR8472910_2.fastq.gz	Fastqsanger.gz
	SRR8472920_1.fastq.gz	Fastqsanger.gz
	SRR8472920_2.fastq.gz	Fastqsanger.gz
	SRR8472911_1.fastq.gz	Fastqsanger.gz

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Modulation of TGF-B1 mediated fibroblast activation in human vocal fold fibroblast cells by Porcine-Derived Lamina Propria Extracellular Matrix Hydrogel

Project description:Vocal folds (VFs) are exposed to external and internal stimuli that may cause damage leading to structural alterations and compromised function. Extracellular matrix (ECM)-based biomaterials have been explored as bioactive scaffolds to tissue repair. An injectable form of the VF-ECM scaffold would be an ideal biomaterial for minimally invasive delivery to injured VFs. The goal of this study was to develop a hydrogel form of VF lamina propria ECM (VFLP-ECM) and to characterize its properties and in vitro cellular response.

2021-05-25 | GSE125463 | GEO

Transcription in wounded rat vocal folds and vocal fold fibroblasts

Project description:We used microarrays to characterize transcriptome profiles of rat vocal fold tissue following surgical injury (vs. naive tissue); rat vocal fold fibroblasts harvested from scar tissue at the 60 d time point (vs. naive fibroblasts); rat vocal fold scar fibroblasts treated with siRNA against the collagen chaperone protein rat gp46 (vs. scramble siRNA). Adult Fischer 344 rat vocal fold tissue was harvested at 3, 14, and 60 days following surgical injury (control = age-matched naive tissue); rat vocal fold scar fibroblasts were obtained via explant culture of tissue obtained 60 days following surgical injury and harvested at 80% confluence during passage 1 (control = age-matched naive rat vocal fold fibroblasts); rat vocal fold scar fibroblasts were treated for 1 h with 50 nM liposome-delivered siRNA against rat gp46 when 80% confluent at passage 1, cultured for an additional 24 h in fresh media, then harvested (control = rat vocal fold scar fibroblasts treated with 50 nM liposome-delivered scramble siRNA).

2014-10-09 | E-GEOD-62204 | biostudies-arrayexpress

Expression data from rat vocal fold at different periods after vocal fold injury

Project description:Expression data from rat vocal fold at different periods after vocal fold injury

| PRJNA579528 | ENA

Transcription in wounded rat vocal folds and vocal fold fibroblasts

2014-10-09 | GSE62204 | GEO

Expression data from rat vocal fold at different periods after vocal fold injury (miRNA)

Project description:Expression data from rat vocal fold at different periods after vocal fold injury (miRNA)

| PRJNA614953 | ENA

Unraveling the molecular pathobiology of vocal fold systemic dehydration using an in vivo rabbit model

Project description:The purpose of this study is to investigate if acute systemic dehydration impacts the vocal folds at the mRNA level. Rabbits were used as animal model to enable a systematic and rigorous assessment of the pathobiology of vocal fold hydration since they can be manipulated in a physiologically realistic manner. We anticipate that the results of this study will help to elucidate the effects of altered hydration state in response to furosemide on vocal fold epithelium, lamina propria and muscle tissue at the transcriptome level. Together with cellular, structural, and functional techniques that are part of this project, we expect to provide validated scientific recommendations on the adverse effects of dehydration and the therapeutic benefits of hydration.

2020-07-09 | GSE132765 | GEO

Expression data from rat vocal fold at different periods after vocal fold injury

Project description:Vocal cord healing is a dynamic process, and many genes and proteins are involved, which play varying roles at different regeneration stages after injury. Previous studies have shown that inflammatory responses occur at the early stage of vocal cord injury, where the fibroblasts proliferate exuberantly with intensive secretion and deposition of ECM. These activities reach the peak at 3-7 days and their intensity begins to decline 15 days later. A study based on the dermal system has shown that ECM remodeling during the repair of injury can last for several months. However, few studies have been conducted as to the dynamic changes of gene expressions and signaling pathway during the healing process of vocal cord injury. Plotting these changes will facilitate the understanding about the physiological changes during healing and the identification of key time points and target genes in fibrosis formation.

2019-10-26 | GSE139383 | GEO

Expression data from rat vocal fold at different periods after vocal fold injury (miRNA)

Project description:Vocal cord healing is a dynamic process, and many genes and proteins are involved, which play varying roles at different regeneration stages after injury. Previous studies have shown that inflammatory responses occur at the early stage of vocal cord injury, where the fibroblasts proliferate exuberantly with intensive secretion and deposition of ECM. These activities reach the peak at 3-7 days and their intensity begins to decline 15 days later. A study based on the dermal system has shown that ECM remodeling during the repair of injury can last for several months. However, few studies have been conducted as to the dynamic changes of gene and microRNA expressions during the healing process of vocal cord injury. Plotting these changes will facilitate the understanding about the physiological changes during healing and the identification of key time points and target genes and microRNAs in fibrosis formation.

2020-03-25 | GSE147473 | GEO

In vitro evaluation of a basic fibroblast growth factor-containing hydrogel toward vocal fold lamina propria scar treatment.

Project description:Scarring of the vocal fold lamina propria can lead to debilitating voice disorders that can significantly impair quality of life. The reduced pliability of the scar tissue-which diminishes proper vocal fold vibratory efficiency-results in part from abnormal extracellular matrix (ECM) deposition by vocal fold fibroblasts (VFF) that have taken on a fibrotic phenotype. To address this issue, bioactive materials containing cytokines and/or growth factors may provide a platform to transition fibrotic VFF within the scarred tissue toward an anti-fibrotic phenotype, thereby improving the quality of ECM within the scar tissue. However, for such an approach to be most effective, the acute host response resulting from biomaterial insertion/injection likely also needs to be considered. The goal of the present work was to evaluate the anti-fibrotic and anti-inflammatory capacity of an injectable hydrogel containing tethered basic fibroblast growth factor (bFGF) in the dual context of scar and biomaterial-induced acute inflammation. An in vitro co-culture system was utilized containing both activated, fibrotic VFF and activated, pro-inflammatory macrophages (MΦ) within a 3D poly(ethylene glycol) diacrylate (PEGDA) hydrogel containing tethered bFGF. Following 72 h of culture, alterations in VFF and macrophage phenotype were evaluated relative to mono-culture and co-culture controls. In our co-culture system, bFGF reduced the production of fibrotic markers collagen type I, α smooth muscle actin, and biglycan by activated VFF and promoted wound-healing/anti-inflammatory marker expression in activated MΦ. Cumulatively, these data indicate that bFGF-containing hydrogels warrant further investigation for the treatment of vocal fold lamina propria scar. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1258-1267, 2018.

| S-EPMC5862030 | biostudies-literature

Protein turnover during in vitro human vocal fold mucosa remodeling

Project description:We present here the first study evaluating the dynamic proteome changes in the remodeling process of decellularized tissue by reseeded cells using vocal fold mucosa as the model system.

2016-01-06 | PXD002734 | Pride

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