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Maternal Vitamin C regulates DNA demethylation and devlopment of the mouse embryonic germline [soma RNA-seq]

ABSTRACT: Maternal Vitamin C regulates DNA demethylation and devlopment of the mouse embryonic germline [soma RNA-seq]

PROVIDER: PRJNA516595 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8476700.fastq.gz	Fastqsanger.gz
	SRR8476701.fastq.gz	Fastqsanger.gz
	SRR8476702.fastq.gz	Fastqsanger.gz
	SRR8476703.fastq.gz	Fastqsanger.gz
	SRR8476704.fastq.gz	Fastqsanger.gz

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Maternal Vitamin C regulates DNA demethylation and devlopment of the mouse embryonic germline [soma RNA-seq]

Project description:Maternal Vitamin C is required in vivo for proper DNA demethylation and development of fetal germ cells in a mouse model of Vitamin C deficiency. Withdrawal of Vitamin C from the maternal diet does not affect overall embryonic development but leads to defects in the fetal germline, which persist well after Vitamin C re-supply during late gestation. The transcriptome of germ cells from Vitamin C-deficient embryos is remarkably similar to that of embryos carrying a mutation in Tet1, which is responsible for DNA demethylation and activation of regulators of meiosis. In agreement with these results, Vitamin C deficiency leads to an aberrant DNA methylation profile that includes incomplete demethylation of key regulators of meiosis and transposable elements. These findings reveal that deficiency in Vitamin C during gestation recapitulates a mutation in Tet1 and disrupts germline reprogramming and development. Our work further indicate that the embryonic germline is sensitive to perturbations of the maternal diet, providing a potential intergenerational mechanism for adjusting fecundity to environmental quality.

2019-07-01 | GSE125495 | GEO

Maternal Vitamin C regulates DNA demethylation and development of the mouse embryonic germline [RRBS]

2019-07-01 | GSE109746 | GEO

Maternal Vitamin C regulates DNA demethylation and devlopment of the mouse embryonic germline [Cut&Run]

2019-07-01 | GSE125496 | GEO

Maternal Vitamin C regulates DNA demethylation and development of the mouse embryonic germline [RNA-Seq]

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Maternal Vitamin C regulates DNA demethylation and development of the mouse embryonic germline

Project description:Maternal Vitamin C regulates DNA demethylation and development of the mouse embryonic germline

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Maternal Vitamin C regulates DNA demethylation and development of the mouse embryonic germline [RRBS]

Project description:Maternal Vitamin C regulates DNA demethylation and development of the mouse embryonic germline [RRBS]

| PRJNA431944 | ENA

Maternal Vitamin C regulates DNA demethylation and development of the mouse embryonic germline [RNA-Seq]

Project description:Maternal Vitamin C regulates DNA demethylation and development of the mouse embryonic germline [RNA-Seq]

| PRJNA431945 | ENA

Maternal Vitamin C regulates DNA demethylation and devlopment of the mouse embryonic germline [Cut&Run]

Project description:Maternal Vitamin C regulates DNA demethylation and devlopment of the mouse embryonic germline [Cut&Run]

| PRJNA516596 | ENA

Drosophila TRAP soma vs germline

Project description:Drosophila TRAP soma vs germline

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Vitamin C Induces Specific Demethylation of H3K9me2 in Mouse Embryonic Stem Cells via Kdm3a/b

Project description:Histone methylation patterns regulate gene expression and are highly dynamic during development. The erasure of histone methylation is carried out by histone demethylase enzymes. We had previously shown that vitamin C enhances the activity of Tet enzymes in embryonic stem (ES) cells, leading to DNA demethylation and activation of germline genes. We report here that vitamin C induces a remarkably specific demethylation of histone H3 lysine 9 dimethylation (H3K9me2) in ES cells. Vitamin C treatment reduces global levels of H3K9me2, but not other histone methylation marks analyzed, as measured by Western blot, immunofluorescence and mass spectrometry. Vitamin C leads to widespread loss of H3K9me2 at large chromosomal domains as well as gene promoters and repeat elements. Vitamin C-induced loss of H3K9me2 occurs rapidly within 24 hours and is reversible. Importantly, we found that the histone demethylases Kdm3a and Kdm3b are required for vitamin C-induced demethylation of H3K9me2. Moreover, we show that vitamin C-induced Kdm3a/b-mediated H3K9me2 demethylation and Tet-mediated DNA demethylation are independent processes. Lastly, we document Kdm3a/b are partially required for the up-regulation of germline genes by vitamin C. These results reveal a specific role for vitamin C in histone demethylation in ES cells, and document that DNA methylation and H3K9me2 cooperate to silence germline genes in pluripotent cells.

2017-06-30 | GSE84009 | GEO

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