Project description:Apomixis in angiosperms terminates sexual reproduction and produces a clonal egg (apomeiosis), which undergoes parthenogenesis, and an embryo sac, which produces endosperm with or without fertilization. Progeny of apomictic plants are maternal, a property viewed by seed producers as a potential tool for producing hybrid seed. Apomixis does not occur in major crops, but sexual termination and apomeiosis (aposporous embryo sac formation) do occur in certain sorghum lines at low frequencies. We identified in sorghum differentially expressed genes (DEG) and significant gene ontology (GO) categories that differentiated ovules of an aposporous (13.6 %) F2 sib from those of a sexual F2 sib at the megasporocyte to early embryo sac formation stages. GO terms indicated that ovules of the aposporous sib experienced elevated glucose levels. These induced ethylene and glucose signaling cascades that appeared to activate the growth modulator TORC1 by silencing the energy sensor SnRK1 and by producing reactive oxygen species scavengers that silenced the stress sensor SnRK2.
Project description:Sorghum is an important cereal crop, which requires large quantities of nitrogen fertilizer for achieving commercial yields. Identification of the genes responsible for low-N tolerance in sorghum will facilitate understanding of the molecular mechanisms of low-N tolerance, and also facilitate the genetic improvement of sorghum through marker-assisted selection or gene transformation. In this study we compared the transcriptomes of root tissues from seven sorghum genotypes having different genetic backgrounds with contrasting low-N tolerance by the RNAseq deep sequencing data. Several genes were found which are common differentially expressed genes between four low-N tolerant sorghum genotypes (San Chi San, China17, KS78 and high-NUE bulk) and three sensitive genotypes (CK60, BTx623 and low-NUE bulk).
Project description:Sorghum is an important cereal crop, which requires large quantities of nitrogen fertilizer for achieving commercial yields. Identification of the genes responsible for low-N tolerance in sorghum will facilitate understanding of the molecular mechanisms of low-N tolerance, and also facilitate the genetic improvement of sorghum through marker-assisted selection or gene transformation. In this study we compared the transcriptomes of root tissues from seven sorghum genotypes having different genetic backgrounds with contrasting low-N tolerance by the RNAseq deep sequencing data. Several genes were found which are common differentially expressed genes between four low-N tolerant sorghum genotypes (San Chi San, China17, KS78 and high-NUE bulk) and three sensitive genotypes (CK60, BTx623 and low-NUE bulk). RNAseq deep sequencing
Project description:This study compared the proteomic differences of rice sorghum GJH1 and rice sorghum BTx623 during seed development in order to reveal the specific proteins of rice sorghum seed development.
Project description:Identification and relative quantification of proteins present during sorghum malting and in a sorghum malt and barley malt mash and boil measured by SWATH-MS.
Project description:Four small RNA libraries from two contrasting sweet sorghum genotypes were sequenced. In this study, One hundred and ninety-five conserved miRNAs belonging to 56 families and 25 putative novel miRNAs from 28 precursors were identified, among which 38 conserved and 24 novel miRNAs were differentially expressed under Cd stress and/or between H18 and L69. Two groups of them: miR169p/q-nov_23 and miR408 were further focused through the coexpression analysis and might be involved in Cd transport, cytoskeleton activity and cell wall construction by regulating their targets. This study presents new insights into the regulatory roles of miRNAs in Cd accumulation and tolerance in sweet sorghum and will help to develop high-Cd accumulation or high Cd-resistant germplasm of sweet sorghum through molecular breeding and/or genetic engineering approaches.
Project description:Exserohilum turcicum is a fungal pathogen that causes northern corn leaf blight and sorghum leaf blight. However, strains are host-specific, meaning a strain typically only causes disease on either maize or sorghum. This pathosystem provides a unique opportunity to identify the mechanisms underlying host specificity through genomic and transcriptomic studies. To characterize host specificity, we (i) constructed de novo annotated assemblies of one sorghum- (15St008) and one maize-specific (Et52B) E. turcicum strain, ii) identified and characterized structural changes between the two genomes, and (iii) compared differential gene expression. This GEO project contains the gene expression of the sorghum-specific strain (15St008) during in planta infection of sorghum and in axenic cultures.
Project description:As no commercial array is available for sorghum microarray analysis, we designed an array based on the annotation of Sbi1.4 gene set and the available 209,835 sorghum ESTs from the NCBI EST database. The array will be used for investigating the expression divergence between grain and sweet sorghum lines under normal and sucrose treatments