Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Self-renewal capability of hepatocytic parental progenitor cells derived from an adult rat liver is maintained for a long term in the chemically defined medium cultured on lamini111

ABSTRACT: Self-renewal capability of hepatocytic parental progenitor cells derived from an adult rat liver is maintained for a long term in the chemically defined medium cultured on lamini111

PROVIDER: PRJNA520903 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Self-renewal capability of hepatocytic parental progenitor cells derived from an adult rat liver is maintained for a long term in the chemically defined medium cultured on lamini111

Project description:We analyzed gene expression of hepatocytic parental progenitor cells cultured on laminin (LN) 111, LN511 and Matrigel (Mat) in a population of small hepatocytes.

2019-02-05 | GSE126063 | GEO

Hepatocytic parental progenitor cells of rat small hepatocytes

Project description:We analyzed gene expression of hepatocytic parental progenitor cells in a population of small hepatocytes. The hepatocytic parental progenitor cells possess self-renewal capability. These cells maintain the ability to differentiate into mature hepatocytes.

2016-09-08 | GSE86523 | GEO

Self-Renewal Capability of Hepatocytic Parental Progenitor Cells Derived From Adult Rat Liver Is Maintained Long Term When Cultured on Laminin 111 in Serum-Free Medium.

Project description:In this study, we investigated how the ability of hepatocytic parental progenitor cells (HPPCs) to self-renew can be maintained and how laminin (LN) isoforms play an important role in their self-renewal and maturation. Hepatocytes isolated from adult rat livers were cultured on hyaluronic acid to form colonies consisting of CD44+ small hepatocytes, which could be passaged on dishes coated with Matrigel. When second-passage cells were plated on Matrigel, LN111, or LN511, HPPCs appeared on Matrigel and LN111 but not on LN511. We identified two types of cells among the second-passage cells: Small, round cells and large, flat ones were observed on Matrigel, whereas the former and latter ones were specifically attached on LN111 and LN511, respectively. We hypothesized that small and round cells are the origin of HPPC colonies, and the binding to LN111 could be key to maintaining their self-renewal capability. Among the integrins involved in LN binding, integrins α3 and β1 were expressed in colonies on LN111 more than in those on LN511, whereas β4 was more strongly expressed in colonies on LN511. Integrin α3highα6β1high cells could form HPPC colonies on LN111 but not on LN511, whereas integrin α6β1low cells could not on either LN111 or LN511. In addition, neutralizing anti-integrin β1 and anti-LN111 antibodies inhibited the passaged cells' ability to attach and form colonies on LN111 by HPPCs. Matrigel overlay induced second-passage cells growing on LN111 to increase their expression of hepatic functional genes and to form 3-dimensional colonies with bile canalicular networks, whereas such a shift was poorly induced when they were grown onLN511. Conclusion: These results suggest that the self-renewal capability of HPPCs depends on LN111 through integrin β1 signaling.

| S-EPMC6939498 | biostudies-literature

Hepatocytic parental progenitor cells of rat small hepatocytes maintain self-renewal capability after long-term culture.

Project description:The liver has a variety of functions for maintaining homeostasis, and hepatocytes play a major role. In contrast with the high regenerative capacity of mature hepatocytes (MHs) in vivo, they have not been successfully expanded ex vivo. Here we demonstrate that CD44-positive cells sorted from small hepatocyte (SH) colonies derived from a healthy adult rat liver can proliferate on a Matrigel-coated dish in serum-free chemically defined medium; in addition, a subpopulation of the cells can divide more than 50 times in a period of 17 weeks every 4-week-passage. The passage cells retained the capability to recover highly differentiated functions, such as glycogen storage, CYP activity and bile secretion. When Matrigel-treated cells from the third passage were transplanted into retrorsine/partial hepatectomy-treated rat livers, the cells engrafted to differentiate into MHs and cholangiocytes. These results suggest that long-term cultured CD44+ SHs retain hepatocytic characteristics in vitro and the capability to differentiate into hepatocytes and cholangiocytes in vivo. Thus, a newly identified subpopulation of MHs possessing the attributes of hepatocytic stem/progenitor cells can be passaged several times without losing hepatocytic characteristics.

| S-EPMC5387414 | biostudies-literature

Transcription and translation profiling of human HepaRG cells upon hepatocytic differentiation

Project description:Comparison of transcriptional and translational regulation upon hepatocytic diffentiation by Total RNA and polysome bound RNA profiling.

2008-01-23 | E-MEXP-1082 | biostudies-arrayexpress

Transcription profiling of human embryonic stem cells and their differentiated progeny grown in chemically defined conditions

Project description:Comparison of H9 human embryonic stem cells, grown in chemically defined and animal-product free conditions, with their differentiated progeny - mesendodermal progenitors arisen as the result of changes made to chemically defined hESC growth conditions. Contributes to a larger work on the use of chemically defined growth conditions to direct hESC differentiation.

2009-07-31 | E-MEXP-930 | biostudies-arrayexpress

Transcription profling of human embryonic stem cells differentiated into endoderm progenitors in chemically-defined media.

Project description:Human embryonic stem cells differentiated into endoderm progenitors in chemically-defined media.

2010-04-02 | E-MEXP-2373 | biostudies-arrayexpress

Human dental pulp cells cultured in FBS vs. chemically defined conditions

Project description:Human dental pulp cells (hDPCs) are one of the promising resources for regenerative medicine and tissue engineering, including derivation of induced pluripotent stem cells (iPSCs). However, our current protocol uses reagents of animal origin, mainly fetal bovine serum (FBS) with potential risk of infectious diseases and unwanted immunogenicity. This time, we designed a chemically defined protocol to isolate and maintain the growth and differentiation potentials of hDPCs.

2014-12-25 | GSE59493 | GEO

Porcine oocytes matured in a chemically defined medium are transcriptionally active

Project description:The statement that fully-grown porcine oocytes are transcriptionally quiescent is not as strong as before. Currently, we know that there is a difference in the transcription profile of germinal vesicle (GV) and metaphase II (MII) oocytes. The goal of our study was to compare the transcription profile of GV, germinal vesicle breakdown (GVBD), metaphase I (MI), and metaphase (MII) oocytes cultivated in a chemically defined, FLI medium. Oocytes were sequenced and subsequently validated using quantitative reverse transcription polymerase chain reaction (RT-qPCR).

2022-11-11 | GSE217367 | GEO

Gene Expression of F. tularensis LVS in hepatocytic cell line FL83B vs LVS cultured in MH broth

Project description:Transcriptional profiling of LVS grown in hepatocytic cell line FL83B was compared to that of LVS cultued in MH broth to discover genes important for intracellular growth.

2013-01-25 | GSE43731 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data