Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptome analysis of reticulated platelets reveals a prothrombotic profile [miRNA-Seq]

ABSTRACT: Transcriptome analysis of reticulated platelets reveals a prothrombotic profile [miRNA-Seq]

PROVIDER: PRJNA521954 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR8566460.fastq.gz	Fastqsanger.gz
	SRR8566470.fastq.gz	Fastqsanger.gz
	SRR8566451.fastq.gz	Fastqsanger.gz
	SRR8566461.fastq.gz	Fastqsanger.gz
	SRR8566471.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 24

Similar Datasets

Transcriptome analysis of reticulated platelets reveals a prothrombotic profile [miRNA-Seq]

Project description:To compare immature and mature platelet transcriptome Methods: reticulated or immature platelets(RPs) were defined as the platelets with the highest thiazole orange staining intensity (15% highest, RNAhigh) according to previous experiences and mature platelets were defined as the platelet with the 30% lowest thiazole orange staining intensity (RNAlow) and sorted (supplemental Figure S1D). RPs have a prothrombotic transcriptomic profile

2019-09-01 | GSE126447 | GEO

Transcriptome analysis of reticulated platelets reveals a prothrombotic profile

Project description:Transcriptome analysis of reticulated platelets reveals a prothrombotic profile

| PRJNA521951 | ENA

Transcriptome analysis of reticulated platelets reveals a prothrombotic profile [mRNA-Seq]

2019-09-01 | GSE126446 | GEO

Transcriptome analysis of reticulated platelets reveals a prothrombotic profile [mRNA-Seq]

Project description:Transcriptome analysis of reticulated platelets reveals a prothrombotic profile [mRNA-Seq]

| PRJNA521955 | ENA

miRNA expression profile in platelets after ex vivo activation

Project description:miRNA expression profile in platelets after ex vivo activation

| PRJEB4974 | ENA

miRNA expression profile in platelets after ex vivo activation

Project description:We performed microRNA sequencing (miRNA-seq) according to Illumina sequencing protocols. Platelet-rich plasma (PRP) has been obtained by centrifuging whole blood at 160 x g for 15 min at room temperature. Platelets have been activated with 3 different physiologic agonists, namely ADP (20uM), collagen (60ug/mL), or Thrombin Receptor Activating Peptide (TRAP 25uM) under continuous stirring. An aliquot of platelets has been obtained at 120 minutes following ADP, collagen and TRAP and then processed to determine miRNA expression profiles. Time 0, indicating samples before any agonist treatment, was used as baseline. Total RNA was extracted by using mirVana Paris kit (life technologies) and then was subjected to size selection library preparation and retrotranscribed. Obtained cDNA was sequenced with the Illumina HiSeq 1000 sequencer .

2015-04-27 | E-MTAB-2078 | biostudies-arrayexpress

Mass spectrometry analysis reveals developmental differences between platelets derived from neonates and adults

Project description:It has been proposed that developmental differences exist between neonatal and adult platelets. Detailed insight therein is, however, still lacking. We have now compared the platelet protein expression profile of neonates and adults employing a label-free quantitative mass spectrometry approach. In addition, platelet aggregation mediated by thromboxane A2 analog, collagen, and peptide agonists of the protease-activated-receptors 1 and 4 was assessed. Results showed that neonatal platelets effectively aggregate in the presence the employed platelet agonists. In agreement with previous studies, higher concentrations of the agonists were required to initiate aggregation in the neonatal platelets. Mass spectrometry analysis revealed no significant difference in the expression level of critical adhesive platelet proteins like glycoprotein (GP)Ib, integrin αIIbβ3, GPV and GPIX. Neonatal platelets did show reduced expression levels of proteins involved in intracellular signaling, i.e. LYN, MAP3k5 and FAM129A. Several proteins that are known to be related to mitochondrial energy metabolism processes such as oxidative phosphorylation, i.e. NDUFS3, NDUFS8 and NDUFA1 were upregulated in neonates. In conclusion, this study reveals that the platelets derived from neonates and adults are distinct. In particular, developmental changes were observed for proteins that belong to metabolic and energy generation processes.

2021-02-05 | PXD004578 | Pride

MicroRNA expression profile of platelets in sickle cell disease

Project description:Circulating platelets from Sickle cell disease (SCD) patients express distinct gene expression patterns that regulate function. The objective of this study is to identify a role of post-transcriptional regulation of the platelet transcriptional signaling by microRNAs. Comparison of microRNA expression in platelets from SCD patients and control subjects, from 2 cohorts-University of Pittsburgh and National Institutes of Health.

2013-06-20 | E-GEOD-41574 | biostudies-arrayexpress

Thrombocythemic Platelets microRNA Profiling

Project description:Post-transcriptional and translational controls mediated by microRNAs (miRNA) regulate diverse biological processes and disease. We systematically dissected regulatory effects of miRNAs relevant to megakaryocytopoiesis and platelet biology by analyzing expression patterns in 79 subjects with thrombocytosis and healthy controls, and integrated these data with transcriptomic and proteomic platforms. We identified and validated a unique 21-miRNA genetic fingerprint associated with thrombocytosis, and demonstrated that a discrete 3-member subset effectively defines ET phenotypes. The genetic signature includes functional guide and passenger strands of the previously-uncharacterized miR 490 (5p and 3p), both of which displayed restricted, low-level expression in megakaryocytes/platelets (compared to leukocytes), and aberrant expression during thrombocytosis, most profound in essential thrombocythemia (ET). Overexpression of miR 490 in a bilineage differentiation model of megakaryocyte/erythroid progenitor formation was insufficient for hematopoietic stem cell colony differentiation and/or lineage specification. Systematic integration of transcriptomic and mass spectrometric datasets with functional reporter assays identified dishevelled associated activator of morphogenesis 1 (DAAM1) as a unique miR 490 5p protein target demonstrating decreased expression in ET platelets, putatively modulated by translational control (and not by mRNA target degradation). Our data define a dysregulated miRNA fingerprint in thrombocytosis, and collectively support a developmentally-restricted function of miR 490 (and its putative DAAM1 target) to conditions associated with exaggerated megakaryocytopoiesis and/or proplatelet formation. Human platelets from a total of 79 samples in 3 phenotypic groups: essential thrombocythemia (ET, N =27), reactive thrombocytosis (RT, N=22) and healthy controls (NO, N=30) were measured on array platform

2012-11-06 | E-GEOD-39046 | biostudies-arrayexpress

Transcriptome profiling of human ovarian cancer cell lines grown in the presence and absence of washed human platelets

Project description:The objective of the study was to determine the influence of platelets on the transcriptome profile of ovarian cancer cells Ovarian cancer cell lines (SKOV3 and 59M) were co-cultured with human washed platelets for 24 hours prior to RNA extraction. These samples were then analysed on affymetrix human Gene 2.0ST arrays.

2022-07-01 | E-MTAB-9673 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data