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Transcriptome analysis of reticulated platelets reveals a prothrombotic profile [mRNA-Seq]

ABSTRACT: Transcriptome analysis of reticulated platelets reveals a prothrombotic profile [mRNA-Seq]

PROVIDER: PRJNA521955 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8566420.fastq.gz	Fastqsanger.gz
	SRR8566430.fastq.gz	Fastqsanger.gz
	SRR8566440.fastq.gz	Fastqsanger.gz
	SRR8566450.fastq.gz	Fastqsanger.gz
	SRR8566421.fastq.gz	Fastqsanger.gz

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Transcriptome analysis of reticulated platelets reveals a prothrombotic profile [mRNA-Seq]

Project description:To compare immature and mature platelet transcriptome Methods: reticulated or immature platelets(RPs) were defined as the platelets with the highest thiazole orange staining intensity (15% highest, RNAhigh) according to previous experiences and mature platelets were defined as the platelet with the 30% lowest thiazole orange staining intensity (RNAlow) and sorted (supplemental Figure S1D). We detected more than 1700 differentially expressed gene, incluning activation receptors RPs have a prothrombotic transcriptomic profile

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Transcriptome analysis of reticulated platelets reveals a prothrombotic profile

Project description:Transcriptome analysis of reticulated platelets reveals a prothrombotic profile

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Transcriptome analysis of reticulated platelets reveals a prothrombotic profile [miRNA-Seq]

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Project description:Purpose: The aim of this study is to determine the relative expresson levels of mRNA transcripts in wild type platelets Methods: Total RNA was extracted and purified from purified platelets from BALB/c male mice (3 independent samples). Platelet purification was performed as described in Josefsson EC et al, Journal of Experimental Medicine (2011) 208:2017-31. Total RNA (100 ng) was used to generate sequencing libraries for whole transcriptome analysis following Illumina’s TruSeq RNA v2 sample preparation protocol. Completed libraries were sequenced on HiSeq 2000 with TruSeq SBS Kit v3- HS reagents (Illumina) as 100 bp paired-end reads at the Australian Genome Research Facility (AGRF), Melbourne. Reads were aligned to the mouse reference genome mm10 and counts for known genes were obtained using the Rsubread package (version 1.18.0) (Liao et al. 2013; Liao et al. 2014).

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