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MTSS1 Suppresses Expansion and Activity of Mammary Tumor-initiating Cells by Mediating P65 Ubiquitination and Degradation

ABSTRACT: MTSS1 Suppresses Expansion and Activity of Mammary Tumor-initiating Cells by Mediating P65 Ubiquitination and Degradation

PROVIDER: PRJNA522687 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8581330_1.fastq.gz	Fastqsanger.gz
	SRR8581330_2.fastq.gz	Fastqsanger.gz
	SRR8581327_1.fastq.gz	Fastqsanger.gz
	SRR8581327_2.fastq.gz	Fastqsanger.gz
	SRR8581328_1.fastq.gz	Fastqsanger.gz

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MTSS1 Suppresses Expansion and Activity of Mammary Tumor-initiating Cells by Mediating P65 Ubiquitination and Degradation

Project description:We conduct transcriptome comparison of control and Mtss1-overexpressed Py8119 cells to gain genomic insights on the biological processes that Mtss1 is involved in breast cancer cells. Through unsupervised cluster analysis of differentially expressed genes (DEGs), we found 1661 genes were significantly changed in Mtss1 cells. Single Site Analysis and Gene Set Enrichment Analysis show that Mtss1 downregualted numerous NF-κB targeted genes.

2019-10-29 | GSE126606 | GEO

MTSS1 Suppresses NF-kB Activity in Lung Adenocarcinoma through Repression of RelA/p65 Phosphorylation

Project description:Metastasis suppressor 1 (MTSS1) is a 755 amino acid protein found in the cell cytoplasm which binds to actin and promotes cytoskeleton organization and is a known suppressor of lung adenocarcinoma metastasis This study demonstrated that preservation of MTSS1 protein expression in lung adenocarcinoma was associated with a 20% 5-year survival advantage in patients. Furthermore, overexpression of MTSS1 was found to reduce metastasis and disease progression in an in-vivo orthotopic lung adenocarcinoma mouse model. Nuclear factor kappa B (NF-kB), an important nuclear transcription factor, has been shown to be constitutively active in lung adenocarcinoma and strongly associated with the development of metastasis. The NF-kB RelA/p65 subunit is involved in NF-kB heterodimer formation and subsequent nuclear translocation leading to activation of NF-kB responsive gene transcription. Phosphorylation and acetylation of p65 are critical post-translational modifications required for NF-kB activation. In this study, we demonstrate that MTSS1 expression leads to decreased NF-κB mediated gene transcription through inhibition of p65 phosphorylation. These findings uncover a novel mechanism through which MTSS1 may regulate lung adenocarcinoma metastasis by impairment of NF-κB regulated gene transcription.

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MTSS1 Suppresses NF-kB Activity in Lung Adenocarcinoma through Repression of RelA/p65 Phosphorylation

Project description:MTSS1 Suppresses NF-kB Activity in Lung Adenocarcinoma through Repression of RelA/p65 Phosphorylation

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Tripartite motif 8 promotes hepatocellular carcinogenesis by mediating ubiquitination and degradation of hepatocyte nuclear factor 1α

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p65-HaloTag copurifying proteins in human 293T cells

Project description:In effort to develop methodology for targeted top down mass spectrometry of NF kappa B p65 from human cells, we evaluated the utility of HaloTag for purification and analysis of recombinant protein. During our study, two datasets of bottom up LC-MS/MS were generated: one from in-gel digestion of the predominant band following p65-HaloTag purification, another from in-solution digestion of all the proteins present in a p65-HaloTag purification. p65-HaloTag copurifying proteins identified in our datasets include the known interactors c-Rel, NF-kappaB p105, NF-kappaB p100, and NF-kappaB inhibitor beta. Over 100 proteins were identified by at least two peptides using a Mascot ion cut-off score of 30.

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RNA Sequencing looking at differential gene expression between p65+/+ and p65-/- mouse embryonic fibroblasts (MEFs)

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