Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ZFP708 maintains DNA methylation during epigenetic reprogramming in embryos

ABSTRACT: ZFP708 maintains DNA methylation during epigenetic reprogramming in embryos

PROVIDER: PRJNA522994 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR8487510.fastq.gz	Fastqsanger.gz
	SRR8487511.fastq.gz	Fastqsanger.gz
	SRR8487509.fastq.gz	Fastqsanger.gz
	SRR8587550.fastq.gz	Fastqsanger.gz
	SRR8587560.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 80

Similar Datasets

BRD8 maintains glioblastoma by epigenetic reprogramming of the P53 network

Project description:BRD8 maintains glioblastoma by epigenetic reprogramming of the P53 network

| PRJNA665550 | ENA

Promoter DNA methylation couples genome-defense mechanisms to epigenetic reprogramming in the mouse germline

Project description:This SuperSeries is composed of the following subset Series: GSE38305: Promoter DNA methylation couples genome-defense mechanisms to epigenetic reprogramming in the mouse germline (part 1) GSE38306: Promoter DNA methylation couples genome-defence mechanisms to epigenetic reprogramming in the mouse germline (part 2) Refer to individual Series

2012-07-31 | E-GEOD-38307 | biostudies-arrayexpress

Epigenetic and transcriptional aberrations in human pluripotent stem cells reflect differences in reprogramming mechanisms [methylation array]

Project description:Human pluripotent stem cells hold great potential for regenerative medicine, but existing cell types have imitations. Human embryonic stem cells derived from fertilized embryos (IVF-ESCs) are considered the “gold standard”, but are allogeneic to potential recipients. Autologous induced pluripotent stem cells (iPSCs) can be produced from somatic cells by forced expression of pluripotency-associated factors, but are prone to genetic and epigenetic aberrations. To determine whether accumulation of such aberrations is intrinsic to somatic cell reprogramming, or secondary to the reprogramming method, we employed an alternative approach by somatic cell nuclear transfer (SCNT). SCNT-based reprogramming to NT-ESCs is mediated by factors present in oocyte’s cytoplasm, thus mimicking early embryogenesis. We generated genetically matched pluripotent stem cells and conducted genome-wide genetic, epigenetic and transcriptional analyses. We discovered that unlike iPSCs, NT-ESCs have a low burden of de novo copy number variations (CNVs), reflecting superior maintenance of genetic stability. Moreover, DNA methylation and transcriptome profiles of NT-ESCs corresponded closely to those of IVF-ESCs. In contrast, iPSCs harbored methylation abnormalities including residual CpG methylation typical of parental fibroblasts, suggesting incomplete reprogramming. We conclude that human somatic cells can be faithfully reprogrammed to pluripotency by SCNT with the potential to satisfy the clinical requirements for cell replacement therapies. Bisulphite converted DNAs of two IVF-ESCs, two sendai produced iPSC lines, two retro-virus produced iPSC lines, four NT-ESCs, and the parental fibroblast were hybridized to the Illumina Infinium HumanMethylation 450K Beadchip

2014-07-08 | E-GEOD-53060 | biostudies-arrayexpress

BRD8 maintains glioblastoma by epigenetic reprogramming of the P53 network [ChIP-seq]

Project description:BRD8 maintains glioblastoma by epigenetic reprogramming of the P53 network [ChIP-seq]

| PRJNA665554 | ENA

BRD8 maintains glioblastoma by epigenetic reprogramming of the P53 network [RNA-seq]

Project description:BRD8 maintains glioblastoma by epigenetic reprogramming of the P53 network [RNA-seq]

| PRJNA665555 | ENA

BRD8 maintains glioblastoma by epigenetic reprogramming of the P53 network [ATAC-seq]

Project description:BRD8 maintains glioblastoma by epigenetic reprogramming of the P53 network [ATAC-seq]

| PRJNA819459 | ENA

H3K27me3 maintains the transcription program during epigenetic reprogramming in the mouse germ line

Project description:H3K27me3 maintains the transcription program during epigenetic reprogramming in the mouse germ line

| PRJNA592544 | ENA

Small RNA-dependent epigenetic modifications in Arabidopsis thaliana embryos

Project description:We report the survey of two repressive epigenetic marks in hand-dissected mature Arabidopsis embryos. DNA from approximately 2500 embryos was extracted for each samples. DNA was treated with bisulfite and fractionated for whole genome bisulfite sequencing to reveal methylated cytosines. Chromatin was fractionnated and immuno-precipited with either anti-H3K9me2 or anti-H3 to test for histone methylation. Both profiles were compared in different mutant backgrounds to survey how small RNA influence reprogramming in the embryo.

2021-05-26 | GSE147245 | GEO

Epigenetic reprogramming shapes the cellular landscape of schwannoma, DNA Methylation

Project description:Epigenetic reprogramming shapes the cellular landscape of schwannoma, DNA Methylation

| PRJNA917427 | ENA

Epigenetic modification agents improve genomic methylation reprogramming in porcine cloned embryos.

Project description:Incomplete DNA methylation reprogramming in cloned embryos leads to low cloning efficiency. Our previous studies showed that the epigenetic modification agents 5-aza-2'-deoxycytidine (5-aza-dC) or trichostatin A (TSA) could enhance the developmental competence of porcine cloned embryos. Here, we investigated genomic methylation dynamics and specific gene expression levels during early embryonic development in pigs. In this study, our results showed that there was a typical wave of DNA demethylation and remethylation of centromeric satellite repeat (CenRep) in fertilized embryos, whereas in cloned embryos, delayed demethylation and a lack of remethylation were observed. When cloned embryos were treated with 5-aza-dC or TSA, CenRep methylation reprogramming was improved, and this was similar to that detected in fertilized counterparts. Furthermore, we found that the epigenetic modification agents, especially TSA, effectively promoted silencing of tissue specific genes and transcription of early embryo development-related genes in porcine cloned embryos. In conclusion, our results showed that the epigenetic modification agent 5-aza-dC or TSA could improve genomic methylation reprogramming in porcine cloned embryos and regulate the appropriate expression levels of genes related to early embryonic development, thereby resulting in high developmental competence.

| S-EPMC4219995 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data