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Human ESC-embryoid body sorted populations: TMRM-hi and ALCAM-low vs. TMRM-hi and ALCAM-hi

ABSTRACT: Human ESC-embryoid body sorted populations: TMRM-hi and ALCAM-low vs. TMRM-hi and ALCAM-hi

PROVIDER: PRJNA523383 | ENA |

REPOSITORIES: ENA

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Human ESC-embryoid body sorted populations: TMRM-hi and ALCAM-low vs. TMRM-hi and ALCAM-hi

Project description:Signal differences between TMRM-hi and ALCAM-low vs. TMRM-hi and ALCAM-hi in representative cardiac and hepatic gene expression in mRNA microarray analysis. INTRODUCTION: Non-genetic purification methods for pluripotent stem cell-derived hepatocyte-like cells are useful for liver regenerative therapy and pharmaceutical applications. METHODS: Fluorescent activated cell sorting (FACS) was used to separate cells by combining two parameters: cellular mitochondrial content evaluated by the mitochondrial membrane potential-dependent fluorescent probe (TMRM) and immunocytochemical detection of activated leukocyte cell adhesion molecule (ALCAM). This method was applied to murine fetal, human embryonic stem cell (ESC)-derived, and human induced pluripotent stem cell (iPSC)-derived cell-mixtures. Separately sorted cell fractions were evaluated by quantitative PCR, immunohistochemistry, and cytochemistry for HNF4a, AFP, and albumin mRNA and/or protein expression. RESULTS: Hepatocyte-like cells were highly segregated into the high TMRM signal and ALCAM-positive population. The purity of hepatocyte-like cells derived from human iPSCs was 97 ± 0.38% (n = 5). CONCLUSIONS: This hepatocyte-like cell purification method may be applicable to quality control of cells for liver regenerative cell therapy and pharmaceutical development.

2020-03-01 | GSE126812 | GEO

4DNESB7XYI9V - sci-Hi-C on mESCs differentiated to embryoid body

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Project description:Chromatin organisation of trophoblast stem cells (TSC) were compared with that of embryonic stem cells (ESC). The method enriches Hi-C libraries, to detect promoter interactions at restriction fragment level. We prepared Hi-C libraries from TSC and ESC (serum grown) samples and enriched them with a promoter capture bait system that captures ~22.000 promoters. Promoter interactions were then analysed using the GOTHiC pipeline.

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Project description:4DNESCX7WHJ1 - sci-RNA-seq on mESCs differentiated to embryoid body

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Promoter Capture Hi-C of mouse ESC and TSC

Project description:Promoter Capture Hi-C of mouse ESC and TSC

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Real-Time PCR analysis of Embryoid Body differentiation of Human pluripotent stem cells

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