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Expression data from 3D cell-printed human bone marrow derived mesenchymal stem cells (hBMMSCs) with liver, heart, skin and cornea decellularized extracellular matrix (dECM) bioinks

ABSTRACT: Expression data from 3D cell-printed human bone marrow derived mesenchymal stem cells (hBMMSCs) with liver, heart, skin and cornea decellularized extracellular matrix (dECM) bioinks

PROVIDER: PRJNA523586 | ENA |

REPOSITORIES: ENA

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Expression data from 3D cell-printed human bone marrow derived mesenchymal stem cells (hBMMSCs) with liver, heart, skin and cornea decellularized extracellular matrix (dECM) bioinks

Project description:Whole transcriptome profiling of hBMMSCs printed with various dECM bioinks were conducted to reveal the effect of compositional variations in a tissue-specific manner Differential expression patterns of genes were observed in a tissue-specific manner, and this research provides strong evidence of the tissue-specific functionalities of dECM bioinks.

2019-02-22 | GSE126877 | GEO

Extracellular matrix hydrogel derived from decellularized tissues enables endoderm organoids culture

Project description:Organoids have huge therapeutic potential and are increasingly opening up new avenues within regenerative medicine. However, the clinical application is greatly limited by the lack of effective GMP-complaint systems for organoid expansion in culture. Here, we envisage that the use of extracellular matrix hydrogels derived from decellularized tissues (DT) can provide the instructive native environment necessary to direct cell growth. These gels possess the biochemical signature of tissue-specific ECM, and have the potential for clinical translation due to their natural, non-carcinogenic origin. We show that gels developed from decellularized porcine small intestinal mucosa have similar physiological range and mechanical properties to commercially available gels. ECM-derived gels enable formation and growth of endoderm-derived organoids and, moreover, supports in vivo organoid growth. The development of these ECM-derived hydrogels opens up the potential for human organoids to be used clinically.

2019-11-05 | GSE135108 | GEO

Extracellular matrix hydrogel derived from decellularized tissues enables endoderm organoids culture

Project description:Organoids have extensive therapeutic potential and are increasingly opening up new avenues within regenerative medicine. However, the clinical application is greatly limited by the lack of effective GMP-compliant systems for organoid expansion in culture. Here, we envisage that the use of extracellular matrix hydrogels derived from decellularized tissues (DT) can provide an environment capable of directing cell growth. These gels possess the biochemical signature of tissue-specific ECM, and have the potential for clinical translation. Gels from decellularized porcine small intestinal mucosa/submucosa enable formation and growth of endoderm-derived human organoids, such as gastric, liver, pancreas, and small intestine. ECM gels could be used for direct organoids derivation from human biopsies, multiple passages with transcriptomic signature comparable to gold standard culture system, and in vivo organoid delivery. The development of these ECM-derived hydrogels opens up the potential for human organoids to be used clinically.

2019-11-05 | GSE138611 | GEO

Extracellular matrix hydrogel derived from decellularized tissues enables endoderm organoids culture

Project description:Extracellular matrix hydrogel derived from decellularized tissues enables endoderm organoids culture

| PRJNA557448 | ENA

Extracellular matrix hydrogel derived from decellularized tissues enables endoderm organoids culture

Project description:Extracellular matrix hydrogel derived from decellularized tissues enables endoderm organoids culture

| PRJNA576507 | ENA

Porcine brain tissue-derived decellularized extracellular matrix LC-MS/MS

Project description:The protein composition of porcine brain tissue-derived decellularized extracellular matrix was identified.

2023-11-24 | PXD046191 | Pride

An extracellular matrix-driven positive feedback loop regulates translation in Idiopathic Pulmonary Fibrosis

Project description:Analysis of gene expression of lung fibroblasts seeded onto decellularized extracellular matrix (ECM). Experiment had 2x2 design where fibroblasts from idiopathic pulmonary fibrosis (IPF) or control patients were seeded onto decelluarized lung tissue from IPF or control patients allowing for determination of gene expression differences that were driven by IPF ECM and which differences were driven by the IPF fibroblast. Lung fibroblasts from 5 patients with idiopathic pulmonary fibrosis and 5 control patients were cultured on decellularized ECM from IPF or control lung. Total RNA and polyribosome RNA were isolated after the cells were cultured on the decellularized ECM for 18 hours. When possible, a control cell line and a diseased cell line were cultured (and processed) simultaneously to minimize the effect of experimental variance induced by running the experiment at different times.Samples with the same batch number (provied in the sample 'characteristics' field) were cultured and processed at the same time.

2014-03-04 | E-GEOD-45686 | biostudies-arrayexpress

Delineation of in vitro chondrogenesis of human synovial stem cells following preconditioning with decellularized matrix

Project description:As a tissue-specific stem cell for chondrogenesis, synovium-derived stem cells (SDSCs) are a promising cell source for cartilage repair. However, a small biopsy can only provide a limited number of cells. Cell senescence from both in vitro expansion and donor age presents a big challenge for stem cell based cartilage regeneration. Here we found that expansion on decellularized extracellular matrix (dECM) full of three-dimensional nanostructured fibers provided SDSCs with unique surface profiles, low elasticity but large volume as well as fibroblast-like shape. dECM expanded SDSCs yielded large pellets with intensive staining of type II collagen and sulfated GAGs, which was supported by both biochemical data and real-time PCR results. Our results also hint at lower levels of inflammatory genes and how they might be responsible for enhanced chondrogenic differentiation in dECM expanded SDSCs. Despite an increase of type X collagen in chondrogenically induced cells, dECM expanded cells had significantly lower potential for endochondral bone formation. Both Wnt and MAPK signals were actively involved in both expansion and chondrogenic induction of dECM expanded cells. dECM expanded human SDSCs could be a potential cell source for autologous cartilage repair Adult human synovial fibroblasts (4 donors, two male and two female, average 43 years old, all had no known joint disease) were grown for one passage on plastic flasks (P cells) or plastic flasks pre-coated with decellularized extracellular matrix (E cells). Aliquots were centrifuged and pellets cultured for 35 days in serum-free chondrogenic medium (P pellet or E pellet). There are no replicates.

2015-06-04 | E-GEOD-69531 | biostudies-arrayexpress

A 3D printed plastic frame deeply impacts yeast cell growth

Project description:Solid State Fermentation (SSF) processes have been explored for yeast growth and protein and metabolites production. However, most of these processes lack standardization. In this work, we present a polylactic acid (PLA) 3D printed matrix that dramatically enhances yeast growth when embedded in liquid media compared to equivalent static cultures, and changes yeast expression patterns at the proteome level. Moreover, differences in sugar assimilation and ethanol production, as the main product of alcoholic fermentation, are observed. Our results suggest that these matrixes may be useful for a vast range of biotechnological applications based on yeast fermentation.

2023-09-07 | PXD043759 | Pride

Decellularized Spinal Cord Matrix Manipulate Glial Niche Alteration via Serglycin Mediated Signalling Pathway [scRNA-seq]

Project description:The decellularized spinal cord matrix (DSCM) provides the advantage in the translational potential for microenvironment replacement therapies to repair spinal cord injury (SCI) However, the cell type alteration in the SCI repairing niche is undefined. Using single cell transcriptome and bulk RNA-seq analysis, we showed that DSCM regulated cell fate changes in niches related to the multicellular model. This work opens the door for understanding the SCI repairing from a tissue niche perspective.

2022-12-31 | GSE202731 | GEO

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