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Transcriptome sequencing of the developing internode and ligular region of maize mutant Sdw3

ABSTRACT: Transcriptome sequencing of the developing internode and ligular region of maize mutant Sdw3

PROVIDER: PRJNA524800 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8645930_1.fastq.gz	Fastqsanger.gz
	SRR8645930_2.fastq.gz	Fastqsanger.gz
	SRR8645931_1.fastq.gz	Fastqsanger.gz
	SRR8645931_2.fastq.gz	Fastqsanger.gz
	SRR8645932_1.fastq.gz	Fastqsanger.gz

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Transcriptome sequencing of the developing internode and ligular region of maize mutant Sdw3

Project description:The reduced plant height and enlarged leaf angle of Sdw3 are mainly attributed to a reduction in longitudinal cell elongation in the internode and an increase in the auricle. To investigate the transcriptome changes during internode and auricle development in Sdw3 versus N18, we performed RNA sequencing analysis.

2020-07-08 | GSE127448 | GEO

Comparative transcriptome analysis of abscisic acid (ABA) deficient mutant (vp5) and normal maize (Vp5) embryos during seed maturation

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Analysis of global gene expression in vegetative and reproductive tissues of maize (Zea mays) that differ in accumulation of starch and sucrose

Project description:Background. Carbon allocation between vegetative and reproductive tissues impacts cereal grain production. Despite their great agricultural importance, sink-source relationships have not been fully characterized at early reproductive stages in maize. Here we quantify the accumulation of non-structural carbohydrates and patterns of gene expression in the top internode of the stem and the female inflorescence of maize at the onset of grain filling (reproductive stage R1). Methods. Top internode stem and female inflorescence tissues of the Puma maize inbred line were collected at reproductive stage R1 (without pollination). The accumulation of non-structural carbohydrates was quantified by spectrophotometry. Global gene expression was evaluated in both tissues by RNA sequencing. Results. At reproductive stage R1, the maize female inflorescence accumulates non-structural carbohydrates, notably starch, at higher levels than the top internode of the stem. Gene expression analysis identified 491 genes to be differentially expressed between the female inflorescence and the stem top internode. Gene ontology classification of differentially expressed genes showed enrichment for sucrose synthesis, the light-dependent reactions of photosynthesis, and transmembrane transporters. Our results suggest that sugar transporters play a key role in sugar partitioning in the maize stem and reveal previously uncharacterized differences between the female inflorescence and the top internode of the stem at early reproductive stages.

2021-09-11 | GSE181998 | GEO

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The maize leaf transcriptome

Project description:We have generated over 80 million 32 nt reads generated from RNA samples isolated from the tip and base of a developing Mo17 leaf. A comparision of these data with the maize AGP resulted in the confirmation of approximately 88% of the maize filtered gene set Keywords: Transcriptome analysis

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Transcriptomic analysis of maize developing earshoot, B73 X Mo17 hybrid

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S frugiperda response to BX3 and BX1 maize mutant

Project description:S. frugiperda fed with bx3 maize mutant and its control genotype B73 AND fed with bx1 maize mutant and its control genotype H88. The bx1 mutant is derived from the insertion of a Mu element within the Bx1 gene in a H88 genetic background (Hamilton 1964). Similarly, the bx3 mutant was obtained in the genotype B73 by insertion of a Mu element in the Bx3 gene (Frey, Chomet et al. 1997).

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Gene expression in developing ears of ra3;tpp4 maize mutants compared to wild-type

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Transcriptomic analysis of maize developing earshoot, IBMRIL X IHP1 hybrid

Project description:Maize earshoot is a metabolic sink espcially related to nitrogen metabolism. Studies on the transcriptomic and metabolic changes occuring in earshoot can provide interesting answers about the nitrogen metabolic potential of the maize variety under study. Intermated B73 X Mo17 recombinant inbred lines (IBMRIL) belong to a predominantly used mapping population. Illinois high protein (IHP) line is a metabolic extreme with respect to nitrogen mebolism with around 30% seed protein. Developing earshoots from this genotype grown at nitrogen-deficient and nitrogen-sufficient conditions were sampled, processed and analyzed through microarray technology. IBMRIL population was crossed to IHP to find the allelic interaction related to nitrogen metabolism. Microarray experiment was conducted using the developing earshoots from IBMRIL X IHP1 lines that deffered in their grain yield significantly. Keywords: Yield response

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