Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Paralithodes camtschaticus

ABSTRACT: Juvenile red king crab transcriptomic response to pH and temperature

PROVIDER: PRJNA525256 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR8782630_1.fastq.gz	Fastqsanger.gz
	SRR8782630_2.fastq.gz	Fastqsanger.gz
	SRR8782640_1.fastq.gz	Fastqsanger.gz
	SRR8782640_2.fastq.gz	Fastqsanger.gz
	SRR8782650_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 91

Similar Datasets

Paralithodes camtschaticus

Project description:Red King Crab Larval Response to pH

| PRJNA523816 | ENA

Paralithodes camtschaticus

Project description:Red King Crab Adult Gill, Heart and Cuticle Tissue Response to pH

| PRJNA525257 | ENA

Paralithodes camtschaticus

Project description:Whole genome sequencing reveals substantial genetic structure and evidence of local adaptation in Alaskan red king crab

| PRJNA1178691 | ENA

Project description:Red king crab transcriptomic response to pH and temperature

| PRJNA530190 | ENA

Paralithodes camtschaticus

Project description:Paralithodes camtschaticus "All" Transcriptome

| PRJNA530046 | ENA

Paralithodes camtschaticus

Project description:Paralithodes camtschaticus overview

| PRJNA525160 | ENA

Telemetry data of red king crab (<i>Paralithodes camtschaticus</i>) migrations in a north Norwegian fjord.

Project description:Acoustic telemetry allows for high-resolution, long-term tracking of moving animals. Here, we describe data on the movement patterns of 37 adult red king crab (RKC, Paralithodes camtschaticus) obtained by means of acoustic telemetry. Acoustically tagged RKC were released in Gamvikfjorden (Sørøya, northern Norway) the 24th of May 2016 and tracked until the 1st of November 2016. Individual crabs resided in the fjord for 1-162 days and were recorded 16 - 11,501 times (mean number of records per crab: 2,851). In total, the data set consist of 105,484 pairs of accurate spatio-temporal coordinates. The acoustic receivers (n = 38) deployed close to the seabed were integrated with temperature sensors that continuously recorded the ambient seawater temperature, resulting in 174,154 water temperature recordings. These novel tracking data can be used to investigate the species' migratory behaviour, spatio-temporal habitat selection, and the relative role of their environment and their possible food sources. Moreover, the high-resolution seawater temperature dataset may serve independently as input data in physical-oceanographic models of this sub-Arctic sill fjord.

| S-EPMC8844838 | biostudies-literature

Transcriptome profiling and in silico detection of the antimicrobial peptides of red king crab Paralithodes camtschaticus.

Project description:Endogenous antimicrobial peptides (AMPs) are evolutionarily ancient factors of innate immunity, which are produced by all multicellular organisms and play a key role in their protection against infection. Red king crab (Paralithodes camtschaticus), also called Kamchatka crab, is widely distributed and the best known species of all king crabs belonging to the family Lithodidae. Despite their economic importance, the genetic resources of king crabs are scarcely known and no full-genome sequences are available to date. Therefore, analysis of the red king crab transcriptome and identification and characterization of its AMPs could potentially contribute to the development of novel antimicrobial drug candidates when antibiotic resistance has become a global health threat. In this study, we sequenced the P. camtschaticus transcriptomes from carapace, tail flap and leg tissues using an Illumina NGS platform. Libraries were systematically analyzed for gene expression profiles along with AMP prediction. By an in silico approach using public databases we defined 49 cDNAs encoding for AMP candidates belonging to diverse families and functional classes, including buforins, crustins, paralithocins, and ALFs (anti-lipopolysaccharide factors). We analyzed expression patterns of 27 AMP genes. The highest expression was found for Paralithocin 1 and Crustin 3, with more than 8,000 reads. Other paralithocins, ALFs, crustins and ubiquicidins were among medium expressed genes. This transcriptome data set and AMPs provide a solid baseline for further functional analysis in P. camtschaticus. Results from the current study contribute also to the future application of red king crab as a bio-resource in addition to its being a known seafood delicacy.

| S-EPMC7391757 | biostudies-literature

Differences in PAR-2 activating potential by king crab (Paralithodes camtschaticus), salmon (Salmo salar), and bovine (Bos taurus) trypsin.

Project description:BACKGROUND: Salmon trypsin is shown to increase secretion of the pro-inflammatory cytokine interleukin (IL)-8 from human airway epithelial cells through activation of PAR-2. Secretion of IL-8 induced by king crab trypsin is observed in a different concentration range compared to salmon trypsin, and seems to be only partially related to PAR-2 activation. This report aim to identify differences in the molecular structure of king crab trypsin (Paralithodes camtschaticus) compared to salmon (Salmo salar) and bovine trypsin (Bos taurus) that might influence the ability to activate protease-activated receptor-2 (PAR-2). RESULTS: During purification king crab trypsin displayed stronger binding capacity to the anionic column used in fast protein liquid chromatography compared to fish trypsins, and was identified as a slightly bigger molecule. Measurements of enzymatic activity yielded no obvious differences between the trypsins tested. Molecular modelling showed that king crab trypsin has a large area with strong negative electrostatic potential compared to the smaller negative areas in bovine and salmon trypsins. Bovine and salmon trypsins also displayed areas with strong positive electrostatic potential, a feature lacking in the king crab trypsin. Furthermore we have identified 3 divergent positions (Asp196, Arg244, and Tyr247) located near the substrate binding pocket of king crab trypsin that might affect the binding and cleavage of PAR-2. CONCLUSION: These preliminary results indicate that electrostatic interactions could be of importance in binding, cleavage and subsequent activation of PAR-2.

| S-EPMC3733831 | biostudies-literature

Paralithodes camtschaticus isolate:MSLRKC-1

Project description:Paralithodes camtschaticus isolate:MSLRKC-1 Genome sequencing and assembly

| PRJNA704614 | ENA