Intergenerational Impact of Genetic and Psychological Factors on Blood Pressure
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA525921 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Intergenerational Impact of Genetic and Psychological Factors on Blood Pressure
| PRJNA525922 | ENA
Project description:BackgroundAlthough studies show that genomics and environmental stressors affect blood pressure, few studies have examined their combined effects, especially in African Americans.ObjectiveWe present the recruitment methods and psychological measures of the Intergenerational Impact of Genetic and Psychological Factors on Blood Pressure (InterGEN) study, which seeks to investigate the individual and combined effects of genetic (G) and environmental (E) (psychological) stressors on blood pressure in African American mother-child dyads. Genetic methods are presented elsewhere, but here we present the recruitment methods, psychological measures, and analysis plan for these environmental stressors.MethodsThis longitudinal study will enroll 250 mother-child dyads (N = 500). Study participation is restricted to women who (a) are ≤21 years of age, (b) self-identify as African American or Black, (c) speak English, (d) do not have an identified mental illness or cognitive impairment, and (e) have a biological child between 3 and 5 years old. The primary environmental stressors assessed are parenting stress, perceived racism and discrimination, and maternal mental health. Covariates include age, cigarette smoking (for mothers), and gender (for children). The study outcome variables are systolic and diastolic blood pressure.AnalysisThe main analytic outcome is genetic-by-environment interaction analyses (G × E); however, main effects (G) and (E) will be individually assessed first. Genetic (G) and interaction analyses (G × E) are described in a companion paper and will include laboratory procedures. Statistical modeling of environmental stressors on blood pressure will be done using descriptive statistics and generalized estimating equation models.ImplicationsThe methodology presented here includes the study rationale, community engagement and recruitment protocol, psychological variable measurement, and analysis plan for assessing the association of environmental stressors and blood pressure. This study may provide the foundation for other studies and development of interventions to reduce the risk for hypertension and to propose targeted health promotion programs for this high-risk population.
| S-EPMC4930011 | biostudies-literature
Project description:The Intergenerational Impact of Genetic and Psychological Factors on Blood Pressure (InterGEN) study aims to delineate the independent and interaction effects of genomic (genetic and epigenetic) and psychological-environmental (maternally perceived racial discrimination, mental health, and parenting behavior) factors on blood pressure (BP) among African American mother-child dyads over time. The purpose of this article is to describe the two-step genetic and epigenetic approach that will be executed to explore Gene × Environment interactions on BP using a longitudinal cohort design. Procedure for the single collection of DNA at Time 1 includes the use of the Oragene 500-format saliva sample collection tube, which provides enough DNA for both the Illumina Multi-Ethnic Genotyping and 850K EPIC methylation analyses. BP readings, height, weight, percentage of body fat, and percentage of body water will be measured on all participants every 6 months for 2 years for a total of 4 time points. Genomic data analyses to be completed include multivariate modeling, assessment of population admixture and structure, and extended analyses including Bonferroni correction, false discovery rate methods, Monte Carlo approach, EIGENSTRAT methods, and so on, to determine relationships among both main and interaction effects of genetic, epigenetic, and psychological environmental factors on BP.
| S-EPMC5025343 | biostudies-literature
Project description:Bio-psychological factors in inflammatory bowel diseases (LIMBO study)
| PRJEB43193 | ENA
Project description:BackgroundDepression is a risk factor for hypertension, yet few studies have been conducted in African American women.ObjectiveWe conducted a secondary analysis of depressive symptoms and high blood pressure among African American women from the Intergenerational Impact of Genetic and Psychological Factors on Blood Pressure longitudinal study (N = 250).MethodsLogistic regression was used to examine depressive symptoms and blood pressure, adjusting for education, employment, and racism/discrimination. Growth curve modeling was used to investigate longitudinal associations between depressive symptoms and systolic (SBP) and diastolic (DBP) blood pressures at 4 time points (T1-T4).ResultsDepressive symptoms at baseline were not prospectively associated with hypertension prevalence. Participants with Beck Depression Inventory scores higher than 10 had higher estimated marginal SBP and DBP over time compared with participants with lower scores.ConclusionDepressive symptoms were not associated with hypertension prevalence at T4, but they were associated with higher estimated marginal SBP and DBP. Future research is needed to elucidate mechanisms and implications for clinical care and prevention.
| S-EPMC8443694 | biostudies-literature
Project description:IntroductionExperiencing psychosocial stress is associated with poor health outcomes such as hypertension and obesity, which are risk factors for developing cardiovascular disease. African American women experience disproportionate risk for cardiovascular disease including exposure to high levels of psychosocial stress. We hypothesized that psychosocial stress, such as perceived stress overload, may influence epigenetic marks, specifically DNA methylation (DNAm), that contribute to increased risk for cardiovascular disease in African American women.MethodsWe conducted an epigenome-wide study evaluating the relationship of psychosocial stress and DNAm among African American mothers from the Intergenerational Impact of Genetic and Psychological Factors on Blood Pressure (InterGEN) cohort. Linear mixed effects models were used to explore the epigenome-wide associations with the Stress Overload Scale (SOS), which examines self-reported past-week stress, event load and personal vulnerability.ResultsIn total, n = 228 participants were included in our analysis. After adjusting for known epigenetic confounders, we did not identify any DNAm sites associated with maternal report of stress measured by SOS after controlling for multiple comparisons. Several of the top differentially methylated CpG sites related to SOS score (P < 1 × 10-5), mapped to genes of unknown significance for hypertension or heart disease, namely, PXDNL and C22orf42.ConclusionsThis study provides foundational knowledge for future studies examining epigenetic associations with stress and other psychosocial measures in African Americans, a key area for growth in epigenetics. Future studies including larger sample sizes and replication data are warranted.
| S-EPMC9554129 | biostudies-literature
Project description:Intergenerational genetic effects on the mouse transcriptome
| PRJEB70518 | ENA
Project description:Intergenerational genomic DNA methylation patterns in mouse hybrid strains Reduced representation bisulfite sequencing from mouse liver, using MspI restriction enzyme, and selecting ~100-300bp size fraction.
2013-12-30 | E-GEOD-53714 | biostudies-arrayexpress
Project description:Blood plasma was collected from 9 naval aviation students 24 hours prior to and no more than 20 minutes after Modular Egress Training (psychological stressor).
2009-12-01 | PRD000158 | Pride
Project description:Chronic psychological stress become synonymous with modern life. It can disrupt homeostasis and lead to numerous health risks. However, the molecular basis is still in its infancy. Here, we employed a chronic restraint stress (CRS) mouse model to investigate the effects of psychological stress on metabolism, behavior, and fertility. Subsequently, we conducted a transcriptome analysis on the liver, brain, and testes, aiming to understand the molecular basis of CRS-induced physiological and behavioral alterations. The results showed that two consecutive weeks of CRS treatment led to delayed growth in mice, abnormalities in blood glucose metabolism, anxiety-like behaviors, and a decline in semen quality. Transcriptome analysis of 18 samples obtained from liver, brain and testis of both control and CRS-treated micerevealed significant changes in gene expression in the liver, brain, and testes. Gene function enrichment analysis, protein and protein interaction analysis, and gene set enrichment analysis highlighted that differentially expressed genes (DEGs) in the liver were primarily involved in the synthesis, metabolism, and transport of fatty acids and ribose, closely associated with glucose metabolism, insulin resistance, the synthesis and secretion of growth hormones, and extensively linked to adaptive immune responses. In the brain, DEGs were not only related to hormone secretion and cognitive function alterations but also played a part in regulating insulin secretion and the development of the reproductive system, closely linked to abnormalities in blood sugar metabolism and semen parameters. In the testes, DEGs mainly concerned immune responses, DNA damage, and hormone regulation, closely associated with abnormalities in semen parameters. These observations suggested that the multitude of health risks stemming from psychological stress-induced homeostatic imbalance could be mediated through the impact on gene expression profiles across various tissues. Despite the challenges in investigating the effects of psychological stress on human tissues and organs, with a reliance on animal models for insights, the role of psychological stress as a contributing factor to male metabolism issues, emotional disturbances, and infertility issues is undeniable. Highlighting its health implications among humans is essential. The study emphasizes the need for further research to explore the broader implications of stress on human health and the potential for intergenerational effects of stress-induced disorders.
2025-07-09 | GSE271865 | GEO