Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA sequencing analysis of ex vivo expanded CD27+CD70- and CD27-CD70+ human regulatory T cells

ABSTRACT: RNA sequencing analysis of ex vivo expanded CD27+CD70- and CD27-CD70+ human regulatory T cells

PROVIDER: PRJNA530612 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR8839460_1.fastq.gz	Fastqsanger.gz
	SRR8839460_2.fastq.gz	Fastqsanger.gz
	SRR8839470_1.fastq.gz	Fastqsanger.gz
	SRR8839470_2.fastq.gz	Fastqsanger.gz
	SRR8839480_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 48

Similar Datasets

RNA sequencing analysis of ex vivo expanded CD27+CD70- and CD27-CD70+ human regulatory T cells

Project description:Our study shows that after extended stimulation of human CD4+CD25+CD127- Tregs in vitro, a stable cell subset that downregulates CD27 and upregulates CD70 develops. Expanded CD27+CD70- Tregs display a gene profile which closely relates to the previously described “Treg gene signature”, whereas CD27-CD70+ Tregs express some genes associated with Treg gene signature and some genes overexpressed in conventional T cells.

2020-07-10 | GSE129251 | GEO

Transcription profiling by array of mouse wild type or CD27-/- OT-I T cells are stimulated with artficial antigen presenting cell lines expressing CD70 and/or CD80

Project description:WT or CD27-/- OT-I T cells are stimulated in vitro with artficial antigen presenting cell (aAPC) lines expressing CD70 and/or CD80 for 2, 4, 8 or 14 hours.

2010-09-29 | E-NCMF-38 | biostudies-arrayexpress

RNAseq of freshly isolated and ex vivo expanded peripheral blood NK cells

Project description:NK cells were isolated from the perpheral blood of healthy donors (n=6) and expanded ex vivo in the presence of feeder cells and IL-2. RNA from fresh and expanded cells was isolated, sequenced, and gene expression of the cell populations were compared.

2021-03-10 | GSE165498 | GEO

CD70 identifies donor derived alloreactive T cells in severe acute Graft vs Host Disease post allogeneic SCT

Project description:Graft-versus-host disease (GvHD) remains a major challenge post allogeneic hematopoietic stem cell transplantation (allo-HSCT) and understanding its immunopathology is crucial for effective therapeutic intervention. Here we investigated the significance of CD70, a co-stimulatory molecule, in the context of aGvHD. CD70 was found to be transiently expressed on activated T cells during early immune reconstitution post-transplantation but persisted longer in aGvHD. Our findings illustrate that CD70+ T cells are characterized by upregulated activation receptors and enhanced cytokine production, while displaying signs of exhaustion. Remarkably, CD70+ T cells expressed chemokine receptors CCR4 and CCR6 and were actively recruited to inflamed tissues, implicating them in aGvHD pathogenesis. The chromatin at transcription factor MGA and NFAT5 loci was found to be selectively altered upon allo-antigenic stimulation in CD70+ T cells ex-vivo. Gene transcriptions of Toll-like receptors and MAPK signalling pathway were also found to differ in CD70+ and CD70- T cells of aGvHD patients. Insights into the clonality and antigen specificity of CD70+ T cell populations revealed potential novel antigenic targets. Further blocking CD70 in allogeneic T cells from aGvHD patients primed with autologous antigen presenting cells prior to HSCT demonstrated that anti-CD70 antibody reduced T cell proliferation and cytokine production. These findings implicate CD70 as a potential therapeutic target to modulate T cell responses and mitigate aGvHD. Targeting the CD70-CD27 co-stimulation pathway holds promise for prophylactic treatment.

2024-10-02 | GSE247452 | GEO

Gene expression signature of ex vivo expanded NK cells for 14 days

Project description:we performed RNA sequencing to employ an unbiased approach to identify the altered gene expression enhancing the function of ex vivo expanded pNK cells for 14 days, and transcriptionally compared day 0 and day 14 pNK cells.

2021-10-27 | GSE166933 | GEO

Gene expression signature of ex vivo expanded NK(CD3-CD56+) cells for 14 days

Project description:We performed RNA sequencing to employ an unbiased approach to identify the altered gene expression enhancing the function of ex vivo expanded pNK cells for 14 days, and transcriptionally compared day 0 and day 14 pNK cells.

2021-10-27 | GSE163538 | GEO

Chronic CD27-CD70 costimulation promotes type 1-specific polarization of effector Tregs

Project description:Most T lymphocytes, including regulatory T cells, express the CD27 costimulatory receptor in steady state conditions. There is evidence that CD27 engagement on conventional T lymphocytes favors the development of Th1 and cytotoxic responses in mice and humans, but the impact on the regulatory lineage is unknown. In this report, we examined the effect of constitutive CD27 engagement on both regulatory and conventional CD4+ T cells in vivo, in the absence of intentional antigenic stimulation. Our data show that both T cell subsets convert into type 1 effectors, characterized by cell activation, cytokine production, response to IFN-and CXCR3-dependent migration to inflammatory sites. Our data highlight the role of the transcription factor Eomes in this process. We conclude that CD27 may regulate the development of Th1 immunity in peripheral tissues as well as the subsequent switch of the effector response into long-term memory.

2022-11-30 | GSE214395 | GEO

Chronic CD27-CD70 costimulation promotes type 1-specific polarization of effector Tregs

Project description:Chronic CD27-CD70 costimulation promotes type 1-specific polarization of effector Tregs

| PRJNA885122 | ENA

RNAseq of freshly isolated and ex vivo expanded peripheral blood NK cells

Project description:RNAseq of freshly isolated and ex vivo expanded peripheral blood NK cells

| PRJNA694692 | ENA

Gene expression signature of ex vivo expanded NK cells for 14 days

Project description:Gene expression signature of ex vivo expanded NK cells for 14 days

| PRJNA702438 | ENA