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Transcriptome Analysis of Plasmodium ΔCSP stable expressing or control HepG2 cells

ABSTRACT: Transcriptome Analysis of Plasmodium ΔCSP stable expressing or control HepG2 cells

PROVIDER: PRJNA530961 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8848060_1.fastq.gz	Fastqsanger.gz
	SRR8848060_2.fastq.gz	Fastqsanger.gz
	SRR8848061_1.fastq.gz	Fastqsanger.gz
	SRR8848061_2.fastq.gz	Fastqsanger.gz
	SRR8848062_1.fastq.gz	Fastqsanger.gz

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Transcriptome Analysis of Plasmodium ΔCSP stable expressing or control HepG2 cells

Project description:Purpose:To understand the change in transcriptome for the overxepression of ΔCSP in HepG2 cells. Methods:Total RNAs of ΔCSP stable expressing or control HepG2 cells were extracted using TRIzol (Invitrogen), following the manufacturer's instructions. RNA-seq and bioinformatic data analysis were performed by Shanghai Sangon Bio Ltd. Briefly, sequencing libraries were generated using VAHTSTM mRNA-seq V2 Library Prep Kit for Illumina® (Vazyme Biotech, Nanjing, China) following manufacturer's recommendations and were sequenced on HiSeq XTen sequencers (Illumina, San Diego, CA). Raw reads in FASTQ format were subjected to quality control using FastQC. RNA-seq reads were aligned to the reference genome using Bowtie. Uniquely mapped reads were used for further analysis. Gene expression levels are expressed as TPM (Transcripts per million reads) and differences in gene expression were calculated with rSeq. Results:There were 492 genes differentially expressed in ΔCSP-overexpressing HepG2 cells compare to the control group respectively (fold change >2 or < 0.5).

2020-12-31 | GSE129323 | GEO

Transcriptome Analysis of Plasmodium CSP stable expressing or control A549 cells

Project description:Transcriptome Analysis of Plasmodium CSP stable expressing or control A549 cells

| PRJNA782537 | ENA

Transcriptome Analysis of Plasmodium CSP stable expressing or control A549 cells

Project description:To investigate the potential effect of overexpression of Plasmodium yoelii cirumsporozoite protein on A549 cells. Control and CSP stable expression A549 cells were constructed by infected with pLenti vector and pLenti-CSP plasmids and cultured with HBSS for 24h. Total RNA from Control and CSP stable expression A549 cells was extracted using the mirVana miRNA Isolation Kit (Ambion) following the manufacturer’s protocol. RNA integrity was evaluated using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). The samples with RNA Integrity Number (RIN) ≥ 7 were subjected to the subsequent analysis. The libraries were constructed using TruSeq Stranded mRNA LTSample Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions. Then these libraries were sequenced on the Illumina sequencing platform (illumina novaseq6000) and 125bp/150bp paired-end reads were generated.

2022-12-31 | GSE189311 | GEO

HBX-HepG2 versus Control-HepG2

Project description:Mature microRNA expression profiling of HBx-expressing HepG2 cells versus Control cells under UV stress The mature microRNA expression profile of HBx-expressing HepG2 cells versus control cells in three independent biological repeated experiments. This study focuses only on human sequences. The complete data matrix is linked below as a supplementary file.

2010-05-16 | E-GEOD-15554 | biostudies-arrayexpress

HBX-HepG2 versus Control-HepG2 (no UV)

Project description:Mature microRNA expression profiling of HBx-expressing HepG2 cells versus Control cells. Free of UV sensitization. The mature microRNA expression profile of HBx-expressing HepG2 cells versus control cells in three independent biological repeated experiments (no UV). This study focuses only on human sequences. The complete data matrix is linked below as a supplementary file.

2010-05-24 | E-GEOD-17474 | biostudies-arrayexpress

miRNAs expression in HepG2-HBx and HepG2-URG11

Project description:Compare microRNAs expression when HepG2 cells stably expressing HBx protein with HepG2 control cells expressing baterial chloramphenicol acetyltransferase Compare microRNAs expression when HepG2 cells stably expressing URG11 protein with HepG2 control cells expressing baterial chloramphenicol acetyltransferase microRNA expression in Cells expressing HBx vs. Cell without HBx microRNA expression in Cells expressing URG11 vs. Cell without URG11

2011-11-23 | E-GEOD-33854 | biostudies-arrayexpress

HBX-HepG2 versus Control-HepG2

Project description:Mature microRNA expression profiling of HBx-expressing HepG2 cells versus Control cells under UV stress

2009-12-31 | GSE15554 | GEO

HBX-HepG2 versus Control-HepG2 (no UV)

Project description:Mature microRNA expression profiling of HBx-expressing HepG2 cells versus Control cells. Free of UV sensitization.

2010-05-24 | GSE17474 | GEO

miRNAs expression in HepG2-HBx and HepG2-URG11

2011-11-23 | GSE33854 | GEO

Control HepG2 vs miR-24 HepG2 cells

Project description:miR-24 targets were identified in this study using mRNA microarrays. Combined with Bioinformatics, our results show that miR-24 regulates cell cycle and DNA repair. Total RNA was isolated from C or miR-24 over-expressing HepG2 cells after 48 hr of transfection and microarray analysis was performed

2009-08-27 | E-GEOD-17828 | biostudies-arrayexpress

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