Project description:Transcriptional profiling of rainbow trout muscle cells comparing muscle cells from small fish with muscle cells from large fish at two time periods. Two-condition experiment, small vs. large-fish muscle cells. Sept. and Dec. spawning fish. Biological replicates: 4 small replicates, 4 large replicates for each time period.

2012-11-29 | E-GEOD-42582 | biostudies-arrayexpress

Oncorhynchus mykiss muscle cells: Small vs. Large fish

Project description:Transcriptional profiling of rainbow trout muscle cells comparing muscle cells from small fish with muscle cells from large fish at two time periods.

2012-11-29 | GSE42582 | GEO

fish metagenome isolate:hybrid fish | breed:fish

Project description:fish metagenome isolate:hybrid fish | breed:fish Metagenome

| PRJNA788359 | ENA

Proteomic characterization of major fish allergy responsive protein parvalbumins in Hilsa (Tenualosa ilisha): A commercially important fish in Southeast Asia

Project description:The protein parvalbumin (PRV)-beta (PRVB) is the primary cause behind food allergies to bony fish. Although PRVB is a well-characterized protein in many bony fishes, little is known about the hilsa, an anadromous fish with great economic importance and mostly found in Southeast Asia. In this study, we have characterized the hilsa PRV utilizing various proteomic approaches in response to two major riverine habitats and developmental stages. Unique peptide sets correspond to three different PRV isoforms were identified in hilsa muscle tissues. Label-free quantitative proteomic analysis coupled with ELISA revealed higher levels of PRVB in young fish comparative to the adult, irrespective of their riverine habitats. A comparative quantitative analysis of PRVB further demonstrated that hilsa had less PRVB than other commonly consumed freshwater fish species. Multiple reaction monitoring (MRM)-based targeted proteomic approach showed the potential of PRV as a marker protein for allergen quantitation and authenticating the presence of hilsa in a complex freshwater fish mixture. Our findings collectively offer fundamental knowledge on hilsa parvalbumins for further investigation on the food safety and quality evaluation of hilsa fish.

2025-01-07 | MSV000096794 | MassIVE

Revealing the transcriptional profile of fish muscle cells in vitro and in vivo: insights to improve fish muscle culture

Project description:In vitro experiments have contributed to numerous fields of knowledge, including fish skeletal muscle. Despite improved strategies, in vitro assays still show discrepancies with in vivo systems, especially for non-model organisms. In this sense, we characterized the transcriptional profile of pacus (Piaractus mesopotamicus) muscle cells in vitro and in vivo. Processes related to proliferation, glycolytic metabolism, and extracellular matrix were enriched in vitro, while energy production, muscle contraction, and amino acid pro-cessing were enriched in vivo. Through qPCR, the genes fn1a (fibronectin 1a), hk1 (hexokinase 1) and ctnnb1 (catenin beta 1), respectively related to extracellular matrix, glycolytic metabo-lism, and cell proliferation and differentiation, were highly expressed in vitro. The genes ckma (creatine kinase, muscle a), acat1 (acetyl-CoA acetyltransferase 1), mdh2 (malate dehydrogenase 2) and pkmb (pyruvate kinase M1/2b), respectively associated with ATP production, fatty acid oxidation, oxidative and glycolytic metabolism, and the genes musk (muscle, skeletal, receptor tyrosine kinase), chrna1 (cholinergic receptor, nicotinic, alpha 1) and clu (clusterin), in-volved in cell signaling, were highly expressed in vivo. Our findings may support the de-velopment of molecular strategies to enhance muscle cell culture technology and expand knowledge for in vitro meat production, improving sustainable fish farming.

2026-03-11 | GSE303536 | GEO

Bacillus altitudinis strain:F7 | isolate:Fish

Project description:Bacillus altitudinis strain:F7 | isolate:Fish Genome sequencing

| PRJNA1051771 | ENA

Salix purpurea strain:Fish Creek | cultivar:Fish Creek

Project description:Salix purpurea strain:Fish Creek | cultivar:Fish Creek Genome sequencing

| PRJNA334202 | ENA