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RNA-sequencing of microglia, T cells and ILC2 from the brains of IL-33 treated aged (20-22mos) mice

ABSTRACT: RNA-sequencing of microglia, T cells and ILC2 from the brains of IL-33 treated aged (20-22mos) mice

PROVIDER: PRJNA533462 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8925430.fastq.gz	Fastqsanger.gz
	SRR8925440.fastq.gz	Fastqsanger.gz
	SRR8925450.fastq.gz	Fastqsanger.gz
	SRR8925431.fastq.gz	Fastqsanger.gz
	SRR8925441.fastq.gz	Fastqsanger.gz

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RNA-sequencing of microglia, T cells and ILC2 from the brains of IL-33 treated aged (20-22mos) mice

Project description:ILC2 are potent producers of IL-5 and IL-13 and Th2 cytokines have been found to promote neurogenesis and cognitive functions. We report microglia, brain T cells and ILC2 have a range of differentially expressed genes in IL-33 treated aged mice. Brain ILC2 upregulated a range of neuroprotective genes.

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scRNA-sequencing of ILC2 sorted from the choroid plexus and meninges of IL-33 treated aged (20-22 mos) mice

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Human ILC2 cells stimulated with IL-25, IL-33, or TSLP

Project description:ILC2 cells are a newly described cell type whose biology and contribution to disease are poorly understood. ILC2 cells are activated by allergens, viral infection, and/or epithelial damage via IL-33 and IL-25. ILC2 cells require IL-2, IL-7, IL-25 and IL-33 for their survival and expansion. In mice, ILC2s produce multiple mediators primarily associated with type 2 inflammation (IL-13, IL-5, IL-4, IL-6, IL-9, IL-10, GM-CSF, amphiregulin). ILC2 cells may contribute to the pathology of asthma through multiple mediators that include IL-13-independent pathways. Our goal is to compare transcriptional profiles of IL-33- or IL-25-activated ILC2 cells from blood to characterize these cells and to identify marker(s) that can be utilized to detect them in human tissue. ILC2 cells (Lineage negative, CRTH2+, CD161+, CD127+) were purified from human blood of 5 different donors by flow cytometry. The ILC2 yield ranged from 20,000 to 165,000 cells per donor (0.001-0.008% WBC). Purified ILC2s were expanded in vitro in the presence of IL-2, IL-7, IL-33 and IL-25 (each at 50 ng/ml) for 7-10 days. Expanded cells maintained the ILC2 phenotype (Lineage negative, CRTH2+, CD161+, CD127+). The cells were rested for 2 days in the presence of 1 ng/ml IL-2 and IL-7 and then treated in the presence of 1 ng/ml IL-2 and IL-7 with either media control, IL-25 (50 ng/ml), IL-33 (50 ng/ml), and/or TSLP (50 ng/ml) in combination, for 6 or 24 hours. Whole RNA was isolated via the RNeasy kit (Qiagen). Stratagene Universal Human Reference RNA was used as the reference.

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Project description:We investigated how aging impacts the ILC2 population in the brain. Through RNA-sequencing of 2-week culture of sorted brain ILC2 from young and aged mice,we identified differential gene expressions regarding cellular exhaustion and self-renewal between young and aged brain ILC2.

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