Project description:In order to identify the genes whose expression was perturbed by ZIP13, we performed a gene expression microarray analysis for the RNAs isolated from WT and ZIP13-depleted human mesenchymal stem cell.
Project description:In order to identify the roles of ZIP7 in hMSCs , we performed a gene expression microarray analysis for the RNAs isolated from WT and ZIP7-depleted hMSCs
Project description:Comprehensive analyses of mRNA expression were performed using skeletal muscle from control and mesenchymal progenitor-depleted mice to investigate the role of mesenchymal progenitors in skeletal muscle maintenance.
Project description:CD133 is a marker of cancer stem cells. DAP5 is a is a translation initiation factor. The goal of the experiment was to characterize the proteomic differences between CD133+/- cells in the WT vs DAP5 depleted background. To this aim, 4 populations of human cells were FACS sorted: shNT_CD133-, shNT CD133+, shDAP5_ CD133-, shDAP5_CD133+. The collected cell pellets were subjected to LC-MS/MS analysis.
Project description:Proteome characterization of mesenchymal stem cells (MSC) and exosomes.
MSCs were cultured in normoxic, hypoxic and in presence of FBS. Exosomes were prepared from normoxic and hypoxic conditions.