Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single-cell RNA-sequencing of spinocerebellar tract neuron populations in cervical and thoracic sections of mouse spinal cord

ABSTRACT: Single-cell RNA-sequencing of spinocerebellar tract neuron populations in cervical and thoracic sections of mouse spinal cord

PROVIDER: PRJNA535483 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR8956042_1.fastq.gz	Fastqsanger.gz
	SRR8956042_2.fastq.gz	Fastqsanger.gz
	SRR8956043_1.fastq.gz	Fastqsanger.gz
	SRR8956043_2.fastq.gz	Fastqsanger.gz
	SRR8956044_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

Bulk RNA-sequencing of spinocerebellar tract neuron populations in cervical and thoracic sections of mouse spinal cord

Project description:We profiled populations of mouse spinocerebellar tract neurons (SCTNs) in cervical and thoracic sections of mouse spinal cord, as defined by retrograde projection labeling. For each sample, SCTNs were labeled by injecting CTB (Alexa555 conjugated form, 1µg/µl in PBS) into the cerebellum using NanojetII at P4 and examined at P6-P7. Labeled SCTNs were collected manually from 150-300µm transverse spinal cords slices were generated using a razor blade. Cells were collected from each injection experiment and then profiled using standard Illumina bulk RNA-seq protocols

2019-04-18 | GSE129948 | GEO

Single-cell RNA-sequencing of spinocerebellar tract neuron populations in cervical and thoracic sections of mouse spinal cord

Project description:We profiled single cells from mouse spinocerebellar tract neurons (SCTNs) in cervical and thoracic sections of mouse spinal cord, as defined by retrograde projection labeling. For each sample, SCTNs were labeled by injecting CTB (Alexa555 conjugated form, 1µg/µl in PBS) into the cerebellum using NanojetII at P4 and examined at P6-P7. Labeled SCTNs were collected manually from 150-300µm transverse spinal cord, and slices were generated using a razor blade. Labelled cells were manually collected from each injection experiment and then profiled using a plate-based SMART-SCRB protocol (see Baek et al. 2019 for detailed methods)

2019-04-26 | GSE130312 | GEO

Bulk RNA-sequencing of spinocerebellar tract neuron populations in cervical and thoracic sections of mouse spinal cord

Project description:Bulk RNA-sequencing of spinocerebellar tract neuron populations in cervical and thoracic sections of mouse spinal cord

| PRJNA533255 | ENA

Transcriptional profile by microRNA microarrays of cervical thymus and thoracic thymus from Foxn1- GFP; Pth-Cre;R26dTomato transgenic mice

Project description:The microRNA oligo microarrays from Agilent were used to determine the miRNAs expression profile of whole organ cervical and thoracic thymi from Foxn1-GFP; Pth-Cre; R26dTomato transgenic mice.

2015-07-31 | E-MTAB-3752 | biostudies-arrayexpress

Transcriptional profile by mRNA microarrays of cervical thymus, thoracic thymus and parathyroid from Foxn1-GFP; Pth-Cre; R26dTomato transgenic mice

Project description:Whole mouse genome microarrays from Agilent were used to determine expression profile of whole organ cervical thymus, thoracic thymus and parathyroid from Foxn1-GFP; Pth-Cre; R26dTomato transgenic mice.

2015-07-31 | E-MTAB-3751 | biostudies-arrayexpress

Molecular Logic of Spinocerebellar Tract Neuron Diversity and Connectivity.

Project description:Coordinated motor behaviors depend on feedback communication between peripheral sensory systems and central circuits in the brain and spinal cord. Relay of muscle- and tendon-derived sensory information to the CNS is facilitated by functionally and anatomically diverse groups of spinocerebellar tract neurons (SCTNs), but the molecular logic by which SCTN diversity and connectivity is achieved is poorly understood. We used single-cell RNA sequencing and genetic manipulations to define the mechanisms governing the molecular profile and organization of SCTN subtypes. We found that SCTNs relaying proprioceptive sensory information from limb and axial muscles are generated through segmentally restricted actions of specific Hox genes. Loss of Hox function disrupts SCTN-subtype-specific transcriptional programs, leading to defects in the connections between proprioceptive sensory neurons, SCTNs, and the cerebellum. These results indicate that Hox-dependent genetic programs play essential roles in the assembly of neural circuits necessary for communication between the brain and spinal cord.

| S-EPMC6555431 | biostudies-literature

Single cell atlas of spinal cord injury in mice reveals a pro-regenerative signature in spinocerebellar neurons

Project description:After spinal cord injury, tissue distal to the lesion contains undamaged cells that could support or augment recovery. Targeting these cells requires a clearer understanding of their injury responses and capacity for repair. Here, we use single nucleus RNA sequencing to profile how each cell type in the lumbar spinal cord changes after a thoracic injury in mice. We present an atlas of these dynamic responses across dozens of cell types in the acute, subacute, and chronically injured spinal cord. Using this resource, we find rare spinal neurons that express a signature of regeneration in response to injury, including a major population that represent spinocerebellar projection neurons. We characterize these cells anatomically and observed axonal sparing, outgrowth, and remodeling in the spinal cord and cerebellum. Together, this work provides a key resource for studying cellular responses to injury and uncovers the spontaneous plasticity of spinocerebellar neurons, uncovering a potential candidate for targeted therapy.

2022-08-01 | GSE172167 | GEO

Human Cortical Kidney Sections

Project description:Human Cortical Kidney Sections

| PRJNA948424 | ENA

Transcriptome analysis of Spinocerebellar Ataxia 17 Aug

Project description:Transcriptome analysis of extracted total RNA from the the cerebellum and muscle of Spinocerebellar Ataxia 17 knock-in mouse model

2015-08-19 | GSE72176 | GEO

Transcriptomic profiling of medulla and cervical spinal cord to study respiratory neuropathology in Spinocerebellar ataxia type 7

Project description:Transcriptomic profiling of medulla and cervical spinal cord to study respiratory neuropathology in Spinocerebellar ataxia type 7

| PRJNA1131307 | ENA

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data