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Single-cell RNA-sequencing of spinocerebellar tract neuron populations in cervical and thoracic sections of mouse spinal cord

ABSTRACT: We profiled single cells from mouse spinocerebellar tract neurons (SCTNs) in cervical and thoracic sections of mouse spinal cord, as defined by retrograde projection labeling. For each sample, SCTNs were labeled by injecting CTB (Alexa555 conjugated form, 1µg/µl in PBS) into the cerebellum using NanojetII at P4 and examined at P6-P7. Labeled SCTNs were collected manually from 150-300µm transverse spinal cord, and slices were generated using a razor blade. Labelled cells were manually collected from each injection experiment and then profiled using a plate-based SMART-SCRB protocol (see Baek et al. 2019 for detailed methods)

ORGANISM(S): Mus musculus

PROVIDER: GSE130312 | GEO | 2019/04/26

REPOSITORIES: GEO

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