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Genome-wide maps of histone crotonylation in pluripotent and endoderm cells. [ChIP-Seq]

ABSTRACT: Genome-wide maps of histone crotonylation in pluripotent and endoderm cells. [ChIP-Seq]

PROVIDER: PRJNA535602 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8959190_1.fastq.gz	Fastqsanger.gz
	SRR8959190_2.fastq.gz	Fastqsanger.gz
	SRR8959200_1.fastq.gz	Fastqsanger.gz
	SRR8959200_2.fastq.gz	Fastqsanger.gz
	SRR8959210_1.fastq.gz	Fastqsanger.gz

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Genome-wide maps of histone crotonylation in pluripotent and endoderm cells. [ChIP-Seq]

Project description:We examined the genome-wide distribution of histone crotonylation and H3K27ac in human embryonic stem cells and induced endoderm cells, by obtaining over four billion bases of sequence from chromatin immunoprecipitated DNA. When compared to H3K27ac, histone crotonylation was more enriched in metabolic genes and in promoters at pluripotent state, while H3k27ac was enriched in the enhancers of endoderm genes. After endoderm differentiation, histone crotonylation and H3K27ac were both enriched in endoderm genes. Moreover, the enrichment of histone crotonylation was more remarkable than that of H3K27ac. Our data indicate that histone crotonylation correlates with endoderm differentiation.

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Project description:Histone lysine crotonylation is a non-acetyl histone lysine modification that is evolutionarily conserved. It plays an important role in regulating various biological processes, including gene transcriptional regulation, spermatogenesis, and cell cycle. However, the dynamic changes and functions of histone crotonylation in preimplantation embryonic development in mammals remain unclear. In this study, we have shown that the transcription coactivator P300 functions as a writer of histone crotonylation during embryonic development. Depletion of P300 resulted in significant developmental defects and dysregulation of the transcriptome of embryos. Importantly, we demonstrated that P300 catalyzes the crotonylation of histone, directly stimulating transcription and regulating gene expression, thereby ensuring successful embryo development to the blastocyst stage. Furthermore, it is showed that the histone H3 lysine 18 crotonylation (H3K18cr) modification is predominantly located in active promoter regions. It serves as an epigenetic hallmark of key transcriptional regulators and promotes the transcription activation of genes. Together, our results propose a model wherein P300-mediated histone crotonylation plays a crucial role in regulating the fate of embryonic development.

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