Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression profile of primary Keratinocytes and E13.5 MEFs

ABSTRACT: Expression profile of primary Keratinocytes and E13.5 MEFs

PROVIDER: PRJNA540597 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Expression profile of primary Keratinocytes and E13.5 MEFs

Project description:We used microarray analysis to study the expression profiles of primary keratinocyes and MEFs. İn an effort to identify transcription factors with epithelial specific expression pattern.

2019-05-02 | GSE130548 | GEO

Transcriptome expression profile of primary mouse embryonic fibroblasts (MEFs) from BAHD1+/+ (WT) and BAHD1-/- (KO) mice

Project description:Comparisons of gene expression profiles of BAHD1-KO MEFs to those of wild-type MEFs (sampled at embryonic day E13.5).

2016-03-15 | GSE73816 | GEO

Expression profile of proliferating and differentiating primary human keratinocytes in vitro

Project description:Human epidermal keratinocytes undergo tightly controlled program of cell differentiation, leading to the formation of cornified envelope. Primary keratinocytes in vitro, under calcium stimulation mimic the differentiation program observed in vivo. Analysis of the transcription profile of two cell population, such as proliferating cells and differentiating cells helps to discover new genes implicated in that process and to understand the mechanisms of regulation of the keratinocyte differentiation. Primary human keratinocytes were cultured under proliferating (Day 0, sub-confluent cells) and differentiating (seven days of high calcium medium) conditions. As a source of cells, we used normal skin from different body sites: back, foreskin, palmoplantar. RNA extracted from cultured primary human keratinocytes were isolated from five different donors. We compared the expression profiles of proliferating versus differentiating keratinocytes.

2010-08-01 | E-GEOD-21413 | biostudies-arrayexpress

Transcriptome expression profile of primary mouse embryonic fibroblasts (MEFs) from BAHD1+/+ (WT) and BAHD1-/- (KO) mice

Project description:Comparisons of gene expression profiles of BAHD1-KO MEFs to those of wild-type MEFs (sampled at embryonic day E13.5). We used DNA microarrays to identify the repertoire of genes differentially expressed by ablation of the BAHD1 gene in mouse embryonic fibroblasts.

2016-03-15 | E-GEOD-73816 | biostudies-arrayexpress

Expression profile of proliferating and differentiating primary human keratinocytes in vitro

2010-08-01 | GSE21413 | GEO

Expression data from human primary keratinocytes

Project description:The genetic expression profile of a Wnt signal agonist, BIO, was evaluated in human primary keratinocytes.

2012-10-01 | GSE40233 | GEO

Gene expression profile of sendai virus infected primary MEFs.

Project description:To investivate the role of mitochondrial-mediate antiviral innate immunity in MEFs, we performed Sendai virus (SeV) infection experiments at the different cultured conditions of the cells. We analyzed the gene expression profile of MEFs that infected or uninfected with SeV either cultured the cells under glucose or galactose medium, and investigate its differences. We found that the both cultured conditions of cells showed normal antiviral responses.

2017-05-31 | GSE75399 | GEO

Expression data from human primary keratinocytes

Project description:The genetic expression profile of a Wnt signal agonist, BIO, was evaluated in human primary keratinocytes. Accelerating scaling up of primary keratinocytes benefits skin autografts for severely burned patients. Wnt signal, a conserved pathway controlling cell cycle and morphogenesis of embryo, has been postulated to promote the cell proliferation and tumorigenesis in adult. Here, the effects of Wnt signal on the growth of interfollicular keratinocytes were investigated. We demonstrated that recombinant Wnt3a significantly promoted the primary keratinocyte growth at a low cell density. A well-characterized GSK-3beta inhibitor, BIO, activated the Wnt signals and also enhanced the colony formation of keratinocytes dose-dependently. Gene expression profile of the BIO-treated keratinocytes revealed the linkage of the BIO with the cell mitosis and indicated that epithelial cell adhesion molecule (EpCAM), a Wnt target gene, was upregulated. Comparing to the EpCAM- keratinocytes, the EpCAM+ cells showed higher proliferation rate and efficacy of the colony formation. Especially, inhibiting the EpCAM expression by shRNA attenuated the proliferation effect of BIO and the growth advantage of the EpCAM+ keratinocytes. These evidences emphasize the positive role of canonical Wnt and EpCAM on the regulation of cell growth and self-renewal for human keratinocytes.

2012-10-01 | E-GEOD-40233 | biostudies-arrayexpress

VZV infection of primary keratinocytes

Project description:VZV infection of primary keratinocytes

| PRJEB4204 | ENA

Global gene expression profile of primary cultured keratinocytes with or without stimulation

Project description:Global gene expression profile of primary cultured keratinocytes with or without stimulation

| PRJNA432151 | ENA